What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?

Name: Bovine Fibronectin Protein, CF
Brand: R&D Systems
SKU: 1030-FN
Rating: 4 (1 reviews)

Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.

Bovine Fibronectin Protein, CF

Catalog #: 1030-FN
14 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
1030-FN-01M
1030-FN-05M

All Fibronectin Proteins and Enzymes

Product Details

Citations (14)

FAQs

Reviews (1)

Summary Product Datasheets Carrier Free Reconstitution Calculator Background Related Research Areas

Bovine Fibronectin Protein, CF Summary

Product Specifications

Purity

>90%, by SDS-PAGE under reducing conditions and visualized by silver stain.

Endotoxin Level

<1.0 EU per 1 μg of the protein by the LAL method.

Activity

Measured by its ability to support cell attachment and spreading when used as a substratum for cell culture. In this application, the recommended concentration for this effect is
1‑5 µg/cm². Fibronectin can also be added in the media to support cell spreading at a concentration of 0.5-50 µg/mL.
Optimal concentration depends on cell type as well as the application or research objectives. Measured by the ability of the immobilized protein to support the adhesion of B16‑F1 mouse melanoma cells. The ED₅₀ for this effect is 10.0-100 ng/mL.

Source

Bovine plasma-derived Fibronectin protein

Product Datasheets

1030-FN

Product Datasheet

COA

SDS

Carrier Free

What does CF mean?

CF stands for Carrier Free (CF). We typically add Bovine Serum Albumin (BSA) as a carrier protein to our recombinant proteins. Adding a carrier protein enhances protein stability, increases shelf-life, and allows the recombinant protein to be stored at a more dilute concentration. The carrier free version does not contain BSA.

What formulation is right for me?

In general, we advise purchasing the recombinant protein with BSA for use in cell or tissue culture, or as an ELISA standard. In contrast, the carrier free protein is recommended for applications, in which the presence of BSA could interfere.

1030-FN

Formulation	Supplied as a 0.2 μm filtered solution in Tris-HCl, NaCl and Urea.
Shipping	The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage:	Use a manual defrost freezer and avoid repeated freeze-thaw cycles. 6 months from date of receipt, -20 to -70 °C as supplied. 1 month, 2 to 8 °C under sterile conditions after opening. Thaw frozen material overnight at 2-8 °C. Do not disturb the product during the initial thaw event. Avoid vortexing and excessive agitation of the product.

Reconstitution Calculator

Background: Fibronectin

Fibronectin is an extracellular matrix (ECM) component and is one of the primary cell adhesion molecules (1). It is composed of multiple homologous repeats and contains many functional domains. The occurrence of different isoforms is due to alternative mRNA splicing of the ED-A, ED-B and III-CS regions and subsequent post-translational modification. Although non-reactive with adhesion receptors in its soluble state, Fibronectin is highly adhesive when on the surface (2). Polymerization of Fibronectin into ECM must be tightly regulated to ensure appropriate adhesive proporties upon ECM formation. Because of its ability to interact with many ligands (e.g. cells, heparin, fibrin, collagen, DNA, immunoglobulin), Fibronectin plays an important role in normal morphogenesis, including cell adhesion, migration, differentiation, and specific gene expression (3 - 6).

References

Vaheri, A. et al. (1978) Biochem. Biophys. Acta. 516:1.
Pytela, R. et al. (1985) Proc. Natl. Acad. Sci. USA 82:5766.
Danen, E.H. et al. (2001) J. Cell. Physiol. 189:1.
Pereira, M. et al. (2001) Am. NY Acad. Sci. 936:438.
Ruoslahti, E. (1999) Adv. Cancer Review 76:1.
Romberger, D.J. (1997) Int. J. Biochem. Cell Biol. 29:939.

Entrez Gene IDs

2335 (Human); 280794 (Bovine)

Alternate Names

CIG; ED-B; fibronectin 1; Fibronectin; FINC; FN; FN1; FNZ; GFND; GFND2; LETS; MSF; SMDCF

Related Research Areas

Citations for Bovine Fibronectin Protein, CF

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

14 Citations: Showing 1 - 10
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GRAF1 Acts as a Downstream Mediator of Parkin to Regulate Mitophagy in Cardiomyocytes
Authors: Zhu, Q;Combs, ME;Bowles, DE;Gross, RT;Mendiola Pla, M;Mack, CP;Taylor, JM;
Cells
Species: S. agalactiae
Sample Types: Whole Cells
Applications: Bioassay
Analysis of the chemotactic factors for tumor-infiltrating fibrocytes and their prognostic significances in lung cancer
Authors: M Tobiume, A Mitsuhashi, A Saijo, H Ogino, T Afroj, H Ogawa, H Goto, S Sato, A Abe, K Haji, R Ozaki, H Takizawa, Y Nishioka
Oncology Letters, 2022-09-30;24(5):417.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Confocal Blood Flow Videomicroscopy of Thrombus Formation over Human Arteries and Local Targeting of P2X7
Authors: P Marchese, M Lombardi, ME Mantione, D Baccellier, D Ferrara, R Chiesa, O Alfieri, C Foglieni
International Journal of Molecular Sciences, 2021-04-14;22(8):.
Species: Human
Sample Types: Whole Tissue
Applications: Bioassay
Variation of Human Neural Stem Cells Generating Organizer States Inï¿½Vitro before Committing to Cortical Excitatory or Inhibitory Neuronal Fates
Authors: N Micali, SK Kim, M Diaz-Busta, G Stein-O'Br, S Seo, JH Shin, BG Rash, S Ma, Y Wang, NA Olivares, JI Arellano, KR Maynard, EJ Fertig, AJ Cross, RW Bürli, NJ Brandon, DR Weinberger, JG Chenoweth, DJ Hoeppner, N Sestan, P Rakic, C Colantuoni, RD McKay
Cell Rep, 2020-05-05;31(5):107599.
Species: Mouse
Sample Types: Whole Cell
Applications: Cell Culture
In vivo monitoring of dynamic interaction between neutrophil and human umbilical cord blood-derived mesenchymal stem cell in mouse liver during sepsis
Authors: SY Ahn, YS Maeng, YR Kim, YH Choe, HS Hwang, YM Hyun
Stem Cell Res Ther, 2020-02-03;11(1):44.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Mitochondrial transfer from mesenchymal stem cells to neural stem cells protects against the neurotoxic effects of cisplatin
Authors: N Boukelmoun, GS Chiu, A Kavelaars, CJ Heijnen
Acta Neuropathol Commun, 2018-12-12;6(1):139.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The plasticity of primary microglia and their multifaceted effects on endogenous neural stem cells in vitro and in vivo
Authors: SU Vay, LJ Flitsch, M Rabenstein, R Rogall, S Blaschke, J Kleinhaus, N Reinert, A Bach, GR Fink, M Schroeter, MA Rueger
J Neuroinflammation, 2018-08-13;15(1):226.
Species: Rat
Sample Types: Whole Cells
Applications: Bioassay
Analysis of Retinoic Acid-induced Neural Differentiation of Mouse Embryonic Stem Cells in Two and Three-dimensional Embryoid Bodies
Authors: J Yang, C Wu, I Stefanescu, A Horowitz
J Vis Exp, 2017-04-22;0(122):.
Species: Mouse
Sample Types: Complex Sample Type
Applications: Bioassay
FGF2 and insulin signaling converge to regulate cyclin D expression in multipotent neural stem cells.
Authors: Adepoju A, Micali N, Ogawa K, Hoeppner D, McKay R
Stem Cells, 2014-03-01;32(3):770-8.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay
The threonine protease activity of testes-specific protease 50 (TSP50) is essential for its function in cell proliferation.
Authors: Li YY, Bao YL, Song ZB, Sun LG, Wu P, Zhang Y, Fan C, Huang YX, Wu Y, Yu CL, Sun Y, Zheng LH, Wang GN, Li YX
PLoS ONE, 2012-05-04;7(5):e35030.
Species: Hamster
Sample Types: Whole Cells
Applications: Adhesion Assay

IL-4 inhibits VLA-4 expression on Tc1 cells resulting in poor tumor infiltration and reduced therapy benefit.
Authors: Sasaki K, Pardee AD, Okada H, Storkus WJ
Eur. J. Immunol., 2008-10-01;38(10):2865-73.
Species: Mouse
Sample Types: Whole Cells
Applications: Bioassay

Age- and dose-related effects on MSC engraftment levels and anatomical distribution in the central nervous systems of nonhuman primates: identification of novel MSC subpopulations that respond to guidance cues in brain.
Authors: Isakova IA, Baker K, Dutreil M, Dufour J, Gaupp D, Phinney DG
Stem Cells, 2007-10-11;25(12):3261-70.
Species: Primate - Macaca mulatta (Rhesus Macaque)
Sample Types: Whole Cells
Applications: Bioassay

Collagen I promotes epithelial-to-mesenchymal transition in lung cancer cells via transforming growth factor-beta signaling.
Authors: Shintani Y, Maeda M, Chaika N, Johnson KR, Wheelock MJ
Am. J. Respir. Cell Mol. Biol., 2007-08-02;38(1):95-104.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

High-level expression of functional chemokine receptor CXCR4 on human neural precursor cells.
Authors: Ni HT, Hu S, Sheng WS, Olson JM, Cheeran MC, Chan AS, Lokensgard JR, Peterson PK
Brain Res. Dev. Brain Res., 2004-09-17;152(2):159-69.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay

FAQs

What is the difference between Bovine Fibronectin Protein, CF (Catalog # 1030-FN) and Human Fibronectin Protein, CF (Catalog # 1918-FN)?
- Only the source species for the protein is different. Both carrier-free proteins have been validated to be bioactive with the same assay protocols listed and recommended concentrations fall within the same range, although customers should determine their optimal concentrations for their particular cell type and application.

View all Proteins and Enzyme FAQs