Canine IL-2 Antibody

Catalog #: AF1815 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1815
AF1815-SP

Cell Proliferation Induced by IL‑2 and Neutralization by Canine IL‑2 Antibody.

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Canine IL-2 Antibody Summary

Species Reactivity

Canine

Specificity

Detects canine IL-2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.2% cross-reactivity with recombinant human IL‑2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant cotton rat IL-2, recombinant bovine IL‑2, recombinant equine IL‑2, and recombinant porcine IL-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant canine IL‑2 (R&D Systems, Catalog # 1815-CL)
Ala21-Thr155 with a Cys147Ser substitution
Accession # Q29416

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Canine IL-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

0.2-0.8 µg/mL

Use in combination with:

Detection Reagent: Canine IL‑2 Biotinylated Antibody (Catalog # BAF1815)

Standard: Recombinant Canine IL-2 (Cys147Ser) Protein (Catalog # 1815-CL)

Neutralization

Measured by its ability to neutralize IL‑2-induced proliferation in the CTLL‑2 mouse cytotoxic T cell line. Gearing, A.J.H. and C.B. Bird (1987) in Lymphokines and Interferons, A Practical Approach. Clemens, M.J. et al. (eds): IRL Press. 276. The Neutralization Dose (ND₅₀) is typically 0.25-1.5 µg/mL in the presence of 10 ng/mL Recombinant Canine IL‑2 (Cys147Ser).

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Cell Proliferation Induced by IL‑2 and Neutralization by Canine IL‑2 Antibody. Recombinant Canine IL-2 (Cys147Ser) (Catalog # 1815-CL) stimulates proliferation in the CTLL-2 mouse cytotoxic T cell line in a dose-dependent manner (orange line). Proliferation elicited by Recombinant Canine IL-2 (Cys147Ser) (10 ng/mL) is neutralized (green line) by increasing concentrations of Canine IL-2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1815). The ND₅₀ is typically 0.25-1.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens. Since then, it has been shown that IL-2 can also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages, and oligodendrocytes. The biological activity of IL-2 is mediated by the binding to cell surface receptor complexes composed of three subunits designated as alpha, beta, and gamma subunits. IL-2 binds the alpha subunit with low affinity. The functional high affinity IL-2 receptor is a heterotrimeric complex of the alpha, beta, and gamma subunits. IL-2 binds with intermediate affinity to the complex containing the beta and gamma subunits, which is also capable of transducing IL-2 signals. In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex. The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, and IL-9. At the amino acid sequence level, canine IL-2 shares 90%, 86%, 85%, 76%, and 75% sequence similarities to feline, human, equine, mouse, and bovine IL-2, respectively.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF