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Cultrex Spheroid Formation Extracellular Matrix, 10X

Catalog #: 3500-096-01
5 Citations Datasheet / COA / SDS
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3500-096-01
Spheroid Cell Invasion Assay_3500-096-K
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Cultrex Spheroid Formation Extracellular Matrix, 10X Summary

Specifications

Shipping Conditions
The product is shipped with dry ice or equivalent. Upon receipt, store it immediately at the temperature recommended below.
Storage
Product is stable for a minimum of 3 months from date of shipment. Store at –80 °C; keep frozen. Dispense into working aliquots if needed; repeated freeze-thaws will destroy product integrity.
Species
Multi-Species

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Scientific Data

N/A Spheroid Cell Invasion Assay_3500-096-K View Larger

Embedding Spheroids in Cultrex BME for Cell Invasion and Migration Assays. MDA-MB-231 (3,000 cells/well) formed spheroids in low adhesion plates for 72 hours using reagents in the Cultrex 3-D Spheroid Basement Membrane Extract Cell Invasion Assay (Catalog # 3500-096-K). Briefly MDA-MB-231 cells were cultured in the presence of 1X Spheroid Formation Matrix (Catalog # 3500-096-01). Then spheroids were embedded in A) no Invasion Matrix, or B) Spheroid Invasion Matrix (Catalog # 3500-096-03). After hydrogel polymerization, DMEM and 10% FBS was added to each well to promote a chemotactic response over a 96 hour period. Note the addition of the Spheroid Invasion Matrix enhanced cell invasion over spheroids cultured without embedding in the invasion matrix.

Citations for Cultrex Spheroid Formation Extracellular Matrix, 10X

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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  1. Cell senescence-associated genes predict the malignant characteristics of glioblastoma
    Authors: C Tan, Y Wei, X Ding, C Han, Z Sun, C Wang
    Cancer Cell International, 2022-12-16;22(1):411.  2022-12-16
  2. PIM1-Induced Cytoplasmic Expression of RBMY Mediates Hepatocellular Carcinoma Metastasis
    Authors: HH Chua, MH Chang, YH Chen, DJ Tsuei, YM Jeng, PH Lee, YH Ni
    Cellular and Molecular Gastroenterology and Hepatology, 2022-10-01;0(0):.  2022-10-01
  3. DOCK6 promotes chemo- and radioresistance of gastric cancer by modulating WNT/beta-catenin signaling and cancer stem cell traits
    Authors: HC Chi, CY Tsai, CS Wang, HY Yang, CH Lo, WJ Wang, KF Lee, LY Lai, JH Hong, YF Chang, MM Tsai, CT Yeh, CH Wu, CC Hsieh, LH Wang, WJ Chen, KH Lin
    Oncogene, 2020-08-04;0(0):.  2020-08-04
  4. Co-delivery of IKBKE siRNA and cabazitaxel by hybrid nanocomplex inhibits invasiveness and growth of triple-negative breast cancer
    Authors: Z Zhao, Y Li, H Liu, A Jain, PV Patel, K Cheng
    Sci Adv, 2020-07-15;6(29):eabb0616.  2020-07-15
  5. Hypoxia Associated Factor (HAF) mediates neurofibromin ubiquitination and degradation leading to Ras-ERK pathway activation in hypoxia
    Authors: Y Song Green, T Sargis, EC Reichert, E Rudasi, D Fuja, E Jonasch, MY Koh
    Mol. Cancer Res., 2019-01-31;0(0):.  2019-01-31

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