DAPT
Chemical Name: (2S)-N-[(3,5-Difluorophenyl)acetyl]-L-alanyl-2-phenyl]glycine 1,1-dimethylethyl ester
Purity: ≥99%
Biological Activity
DAPT is a γ-secretase inhibitor. DAPT reduces Aβ40 and Aβ42 levels in human primary neuronal cultures (IC50 values are 115 and 200 nM for total Aβ and Aβ42 respectively) and in brain extract, cerebrospinal fluid and plasma in vivo. DAPT has no effect on APPα and APPβ levels. DAPT blocks Notch signaling in hybrid human-mouse fetal thymus organ culture (FTOC) and causes ESCs to commit to neuronal differentiation. DAPT can be used in a small molecule cocktail to derive cortical neurons from hPSCs and to maintain hepatocytes in culture. DAPT also promotes the formation of cone photoreceptors in retinal organoids.For more information about how DAPT may be used, see our protocols: Accelerated Induction of Cortical Neurons from hiPSCs, Human iPSC-derived Myoblasts, Generating Midbrain Dopaminergic Neurons from hPSCs.
DAPT synthesized to Ancillary Material Grade also available.
Scientific Data
Dopaminergic Neurons Derived from hiPSCs using DAPT. Dopaminergic neurons derived from human induced pluripotent stem cells (hiPSC) using SB 431542 (Catalog # 1614, Tocris), CHIR 99021 (Catalog # 4423, Tocris), DAPT and Purmorphamine (Catalog # 4551, Tocris). Cells cultured in Neuronal Media supplemented with TGF-3 (Catalog # 8420-B3, R&D Systems), cAMP, GDNF (Catalog # 212-GD, R&D Systems), BDNF (Catalog # 248-BDB, R&D Systems), N21-MAX (Catalog # AR008, R&D Systems). Differentiation shown at days 55 (left) and 62 (right). Images courtesy of Kevin Flynn, Bio-Techne.
Technical Data
The technical data provided above is for guidance only.
For batch specific data refer to the Certificate of Analysis.
Tocris products are intended for laboratory research use only, unless stated otherwise.
Background References
-
The notch response inhibitor DAPT enhances neuronal differentiation in embryonic stem cell-derived embryoid bodies independently of sonic hedgehog signaling.
Crawford and Roelink
Dev.Dyn., 2007;236:886 -
Functional gamma-secretase inhibitors reduce beta-amyloid peptide levels in brain.
Dovey et al.
J.Neurochem., 2001;76:173 -
Different thresholds of notch signaling bias human precursor cells towards B-, NK-, monocytic/dendritic, or T-cell lineage in thymus microenvironment.
De Smedt et al.
Blood, 2005;106:3498 -
SnapShot: Growing Organoids from Stem Cells.
Sato et al.
Cell, 2015;161:1700
Product Datasheets
Reconstitution Calculator
Molarity Calculator
Citations for DAPT
The citations listed below are publications that use Tocris products. Selected citations for DAPT include:
53 Citations: Showing 1 - 10
-
Biphasic activation of WNT signaling facilitates the derivation of midbrain dopamine neurons from hESCs for translational use.
Authors: Kim Et al.
Cell Stem Cell 2021;28:343
-
Preclinical Efficacy and Safety of a Human Embryonic Stem Cell-Derived Midbrain Dopamine Progenitor Product, MSK-DA01
Authors: Piao Et al.
Cell Stem Cell 2021;28:217
-
A Human Pluripotent Stem Cell-based Platform to Study SARS-CoV-2 Tropism and Model Virus Infection in Human Cells and Organoids
Authors: Yang Et al.
Cell Stem Cell 2020;27:125
-
Quantification of human neuromuscular function through optogenetics.
Authors: Vila Et al.
Theranostics 2019;9:1232
-
Long-term functional maintenance of primary human hepatocytes in vitro.
Authors: Xiang Et al.
Science 2019;364:399
-
Neuropathy-causing mutations in HSPB1 impair autophagy by disturbing the formation of SQSTM1/p62 bodies.
Authors: Haidar Et al.
Autophagy 2019;15:1051
-
HSP90-incorporating chaperome networks as biosensor for disease-related pathways in patient-specific midbrain DA neurons.
Authors: Kishinevsky Et al.
Nat Commun 2018;9:4345
-
A stably self-renewing adult blood-derived induced neural stem cell exhibiting patternability and epigenetic rejuvenation.
Authors: Sheng Et al.
Nat Commun 2018;9:4047
-
A biodegradable hybrid inorganic nanoscaffold for advanced stem cell therapy.
Authors: Yang
Nat Commun 2018;9:3147
-
Co-targeting of Tiam1/Rac1 and Notch ameliorates chemoresistance against dox. in a biomimetic 3D lymphoma model.
Authors: Ikram Et al.
Oncotarget 2018;9:2058
-
VGF nerve growth factor inducible is involved in retinal ganglion cells death induced by optic nerve crush.
Authors: Takeuchi Et al.
Sci Rep 2018;8:16443
-
Gain of toxic apolipoprotein E4 effects in human iPSC-derived neurons is ameliorated by a small-molecule structure corrector.
Authors: Wang
Nat Med 2018;24(5):647
-
Hey2 regulates the size of the cardiac progenitor pool during vertebrate heart development.
Authors: Gibb Et al.
Development 2018;145
-
The COPII cargo adapter SEC24C is essential for neuronal homeostasis.
Authors: Wang Et al.
J Clin Invest 2018;128:3319
-
ErbB4 signaling stimulates pro-inflammatory macrophage apoptosis and limits colonic inflammation.
Authors: Schumacher
Cell Death Dis 2017;8:e2622
-
Lactate-activated macrophages induced aerobic glycolysis and epithelial-mesenchymal transition in breast cancer by regulation of CCL5-CCR5 axis: a positive metabolic feedback loop.
Authors: Lin Et al.
Oncotarget 2017;8:110426
-
Specification of murine ground state pluripotent stem cells to regional neuronal populations.
Authors: Alsanie
Sci Rep 2017;7(1):16001
-
Neuroprotective astrocyte-derived Ins/IGF-1 stimulates endocytic processing and extracellular release of neuron-bound Aβ oligomers.
Authors: Pitt Et al.
Mol Biol Cell 2017;28:2623
-
Soluble Gamma-secretase Modulators Attenuate Alzheimer's β-amyloid Pathology and Induce Conformational Changes in Presenilin 1.
Authors: Raven Et al.
EBioMedicine 2017;24:93
-
A modular platform for differentiation of human PSCs into all major ectodermal lineages.
Authors: Tchieu Et al.
Cell Stem Cell 2017;21:399
-
Translocation of the papillomavirus L2/vDNA complex across the limiting membrane requires the onset of mitosis.
Authors: Calton Et al.
PLoS Pathog 2017;13:e1006200
-
Efficient Generation of Hypothalamic Neurons from Human Pluripotent Stem Cells.
Authors: Wang Et al.
Curr Protoc Hum Genet 2016;90:21.5.1
-
Canonical Notch signaling plays an instructive role in auditory supporting cell development.
Authors: Campbell Et al.
Zoological Lett 2016;6:19484
-
Generation of serotonin neurons from human pluripotent stem cells.
Authors: Lu Et al.
Nat Biotechnol 2016;34:89
-
Capturing the biology of disease severity in a PSC-based model of familial dysautonomia.
Authors: Zeltner Et al.
Nat Med 2016;22:1421
-
Notch3 drives development and progression of cholangiocarcinoma
Authors: Guest Et al.
Proc Natl Acad Sci U S A. 2016;113:12250
-
Analysing human neural stem cell ontogeny by consecutive isolation of Notch active neural progenitors.
Authors: Edri Et al.
Nat Commun 2015;6:6500
-
Generation of clinical-grade human induced pluripotent stem cells in Xeno-free conditions.
Authors: Wang Et al.
Stem Cell Res Ther 2015;6:223
-
On the origin of vertebrate somites.
Authors: Onai Et al.
Blood 2015;1:33
-
A small molecule targeting ALK1 prevents Notch cooperativity and inhibits functional angiogenesis.
Authors: Kerr Et al.
Angiogenesis 2015;18:209
-
Neurogenin 3 Expressing Cells in the Human Exocrine Pancreas Have the Capacity for Endocrine Cell Fate.
Authors: Gomez Et al.
Sci Rep 2015;10:e0133862
-
Pleiotropic effects of Sox2 during the development of the zebrafish epithalamus.
Authors: Pavlou Et al.
PLoS One 2014;9:e87546
-
Astrocyte-derived endothelin-1 inhibits remyelination through notch activation.
Authors: Hammond Et al.
Neuron 2014;81:588
-
Canonical Notch signalling is inactive in urothelial carcinoma.
Authors: Greife Et al.
BMC Cancer 2014;14:628
-
Mir-23a and mir-125b regulate neural stem/progenitor cell proliferation by targeting Musashi1.
Authors: Gioia Et al.
Anticancer Res 2014;11:1105
-
Notch signaling regulates nucleocytoplasmic Olig2 translocation in reactive astrocytes differentiation after ischemic stroke.
Authors: Marumo Et al.
Neurosci Res 2013;75:204
-
Generating regionalized neuronal cells from pluripotency, a step-by-step protocol.
Authors: Kirkeby Et al.
Front Cell Neurosci 2013;6:64
-
Common developmental pathways link tooth shape to regeneration.
Authors: Fraser Et al.
Dev Biol 2013;377:399
-
The role of Notch and γ-secretase inhibition in an ovarian cancer model.
Authors: Shah Et al.
J Neurosci 2013;33:801
-
Presenilin1 regulates histamine neuron development and behavior in zebrafish, danio rerio.
Authors: Sundvik Et al.
J Neurosci 2013;33:1589
-
Notch increases the shedding of HB-EGF by ADAM12 to potentiate invadopodia formation in hypoxia.
Authors: Díaz Et al.
J Cell Biol 2013;201:279
-
Inflammation-mediated notch signaling skews fanconi anemia hematopoietic stem cell differentiation.
Authors: Du Et al.
J Immunol 2013;191:2806
-
Shaping organs by a wingless-int/Notch/nonmuscle myosin module which orients feather bud elongation.
Authors: Li Et al.
Proc Natl Acad Sci U S A 2013;110:E1452
-
Opposing effects of bacitracin on human papillomavirus type 16 infection: enhancement of binding and entry and inhibition of endosomal penetration.
Authors: Campos Et al.
J Virol 2012;86:4169
-
Notch signaling inhibits axon regeneration.
Authors: Bejjani and Hammarlund
PLoS One 2012;73:268
-
Fibulin-3 promotes glioma growth and resistance through a novel paracrine regulation of Notch signaling.
Authors: Hu Et al.
Cancer Res 2012;72:3873
-
Jagged1 and Notch1 help edit M cell patterning in Peyer's patch follicle epithelium.
Authors: Hsieh and Lo
Dev Comp Immunol 2012;37:306
-
Macrophage-derived Wnt opposes Notch signaling to specify hepatic progenitor cell fate in chronic liver disease.
Authors: Boulter Et al.
Nat Med 2012;18:572
-
Notch and TGFβ form a reciprocal positive regulatory loop that suppresses murine prostate basal stem/progenitor cell activity.
Authors: Valdez Et al.
RNA Biol 2012;11:676
-
Notch promotes vascular maturation by inducing integrin-mediated smooth muscle cell adhesion to the endothelial basement membrane.
Authors: Scheppke Et al.
Cell Stem Cell 2012;119:2149
-
Characterization of full-length and proteolytic cleavage fragments of desmoglein-2 in native human colon and colonic epithelial cell lines.
Authors: Kolegraff Et al.
Cell Adh Migr 2011;5:306
-
Mindbomb 1, an E3 ubiquitin ligase, forms a complex with RYK to activate Wnt/β-catenin signaling.
Authors: Berndt Et al.
J Cell Biol 2011;194:737
-
Group II metabotropic glutamate receptor stimulation triggers production and release of Alzheimer's amyloid(β)42 from isolated intact nerve terminals.
Authors: Kim Et al.
Neuron 2010;30:3870
FAQs
No product specific FAQs exist for this product, however you may
View all Small Molecule FAQsReviews for DAPT
There are currently no reviews for this product. Be the first to review DAPT and earn rewards!
Have you used DAPT?
Submit a review and receive an Amazon gift card.
$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image
$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image