Equine IL-2 Biotinylated Antibody

Catalog #: BAF1613 Datasheet / COA / SDS

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BAF1613

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Equine IL-2 Biotinylated Antibody Summary

Species Reactivity

Equine

Specificity

Detects equine IL‑2 in ELISAs and Western blots. In sandwich immunoassays, less than 0.3% cross-reactivity with recombinant human IL-2, recombinant mouse IL-2, recombinant rat IL-2, recombinant feline IL-2, recombinant bovine IL-2, recombinant canine IL-2, recombinant cotton rat IL-2, and recombinant porcine IL-2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant equine IL‑2
Ala21-Thr149 (Cys141Ser)
Accession # NP_001078902.1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Equine IL‑2 (Cys141Ser) (Catalog # 1613-IL)

Immunocytochemistry

5-15 µg/mL

See below

Equine IL-2 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Detection (Matched Antibody Pair)

0.1-0.4 Not Assigned

Use in combination with:

Capture Reagent: Equine IL‑2 Antibody (Catalog # AF1613)

Standard: Recombinant Equine IL-2 (Cys141Ser) Protein (Catalog # 1613-IL)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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IL‑2 in Equine PBMCs. IL-2 was detected in immersion fixed equine peripheral blood mononuclear cells (PBMCs) using Goat Anti-Equine IL-2 Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1613) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (red; Catalog # NL999) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: IL-2

Interleukin 2 was initially identified as a T cell growth factor that is produced by T cells following activation by mitogens or antigens (1). IL-2 has since been found to also stimulate the growth and differentiation of B cells, natural killer (NK) cells, lymphocyte activated killer (LAK) cells, monocytes/macrophages and oligodendrocytes (2).

The biological activity of IL-2 is mediated by the binding of IL-2 to cell surface receptor complexes. The functional high-affinity receptor that mediate IL-2 signals is composed of three polypeptide chains, the IL-2 receptor alpha, beta and gamma subunits (3). IL-2 also signals via the intermediate affinity receptor complex of the beta and gamma subunits (4). In T cells, the beta and gamma subunits are shared with the IL-15 receptor complex (5). The gamma subunit of the IL-2 receptor complex has also been shown to be a subunit of the receptor complexes of IL-4, IL-7, IL-9 and IL-21 (6).

At the amino acid sequence level, equine IL-2 shares 72%, 70%, 56% and 54% sequence similarities with human, porcine, rat and mouse IL-2, respectively. It has been reported that equine IL-2 augmented proliferation in equine peripheral blood mononuclear cells, but has no effect on mouse CTLL-2 cells (7).

References

Morgan, D.A. et al. (1976) Science 193:1007.
Smith, K.A. et al. (1988) Science 240:1169.
Taniguchi, T. and Y. Minami (1993) Cell 73:5.
Giri, J. et al. (1994) EMBO J. 13:2822.
Waldmann, T. et al. (1998) Int. Rev. Immunol. 16:205.
Nelson, B.H. and D.M. Willeford (1998) Adv. Immunol. 70:1.
E.V. Vandergrift and D.W. Horohov (1993) Vet. Immunol. Immunopathol. 39:395.

Long Name

Interleukin 2

Entrez Gene IDs

3558 (Human); 16183 (Mouse); 116562 (Rat); 396868 (Porcine); 280822 (Bovine); 403989 (Canine); 100034204 (Equine); 751114 (Feline); 100302458 (Rabbit)

Alternate Names

Aldesleukin; IL2; IL-2; IL-2lymphokine; interleukin 2; interleukin-2; involved in regulation of T-cell clonal expansion; Proleukin; T cell growth factor; T-cell growth factor; TCGF