GABA-A R beta 2 C-Terminus Antibody

Catalog #: PPS031 Datasheet / COA / SDS

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PPS031

Detection of GABAAR beta 2 C-Terminus by Western Blot.

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GABA-A R beta 2 C-Terminus Antibody Summary

Species Reactivity

Human, Mouse, Rat, Bovine, Canine, Primate

Specificity

Human, mouse, rat, and bovine ~50 - 56 kDa GABA_A R beta 2 subunit, C-Terminus

Source

Polyclonal Rabbit IgG

Purification

Antigen Affinity-purified

Immunogen

Fusion protein from the cytosolic loop of the rat GABA_A R beta 2 subunit, C-Terminus

Formulation

100 μL in 10 mM HEPES (pH 7.5), 150 mM NaCl, 100 μg/mL BSA and 50% glycerol.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1:1000 dilution

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of GABA_AR beta 2 C-Terminus by Western Blot. Western blot of 5 - 7 μg rat brain (cerebellum) lysates. The blot was incubated overnight at 2 - 8° C with anti-GABA_AR beta 2 subunit, C-Terminus diluted 1:1000. The antibody labeled the ~50 - 56 kDa beta 2 subunit of the GABA_AR.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

For long-term storage, ≤ -20° C is recommended. Product is stable at ≤ -20° C for at least 1 year.

Background: GABA-A R beta 2

GABA_A ( gamma -aminobutyric acid-type A) receptors are members of the cysteine-loop family of neurotransmitter-gated ion channels. GABA binding to A-type receptors induces anion-selective ion channel opening. These receptors are the principal fast inhibitory neurotransmitter receptors in the CNS. GABA_A receptors are heteropentamer combinations of seven subunit types; alpha, beta, gamma, δ, epsilon, theta, and π. Three subunits, alpha, beta, and gamma, have at least three separate gene products in mammals, and typical GABA_A receptors have some combination of alpha, beta and gamma subunits. The rat beta 2 isoform is a 55 kDa, 450 amino acid (aa), 4 transmembrane protein with two terminal extracellular regions. The ligand-binding region is in the N-terminus (aa 13 - 218). The beta 2 subunit is part of the most common GABA_A receptor combination in the mammalian brain ( alpha 1 beta 2 gamma 2). Like the other two beta subunits, beta 2 is phosphorylated on a consensus phosphorylation site (S410 of the precursor) that exists in the second cytoplasmic domain. In contrast to the other beta sub-units, only PKC is capable of this modification. However, tyrosine phosphorylation of beta 2 is known, and this apparently increases GABA_A current amplitude.

References

Darlison, M.G. et al. (2005) Cell. Mol. Neurobiol. 25:607.
Akabas, M.H. (2004) Int. Rev. Neurobiol. 62:1.
Song, M. and R.O. Messing (2005) Cell. Mol. Life Sci. 62:119.
Brandon, N.J. et al. (2002) J. Neurosci. 22:6353.
McDonald, B.J. et al. (1998) Nat. Neurosci. 1:23.
Wan, Q. et al. (1997) J. Neurosci. 17:5062.

Long Name

gamma-Aminobutyric Acid Type A Receptor, beta-2 Polypeptide

Entrez Gene IDs

2561 (Human); 14401 (Mouse); 25451 (Rat)

Alternate Names

GABA A R beta 2; GABA(A) receptor subunit beta-2; GABAAR beta 2; GABAARb2; GABRB2; gamma-aminobutyric acid (GABA) A receptor, beta 2; gamma-aminobutyric acid A receptor beta 2; gamma-aminobutyric acid receptor subunit beta-2; MGC119386; MGC119388; MGC119389