Home / Activin RIA/ALK-2 / Human Activin RIA/ALK-2 Antibody

Human Activin RIA/ALK-2 Antibody

Catalog #: MAB637
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB637-SP
MAB637-500
MAB637-100
Activin RIA/ALK‑2 in HUVEC Human Cells.
1 Image
Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Activin RIA/ALK-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Activin RIA/ALK‑2 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 71412
Purification
Protein A or G purified from ascites
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Activin RIA/ALK‑2
Asp23-Val124
Accession # Q04771
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Activin RIA/ALK-2 Fc Chimera (Catalog # 637-AR)
under non-reducing conditions only
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry Activin RIA/ALK-2 antibody in HUVEC Human Cells by Immunocytochemistry (ICC). View Larger

Activin RIA/ALK‑2 in HUVEC Human Cells. Activin RIA/ALK-2 was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Mouse Anti-Human Activin RIA/ALK-2 Monoclonal Antibody (Catalog # MAB637) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (orange; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Loading...
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Activin RIA/ALK-2

Activin isoforms and other members of the TGF-beta superfamily exert their biological effects by binding to heteromeric complexes of a type I and a type II serine‑threonine kinase receptor, both of which are essential for signal transduction. To date, seven type I and five type II receptors, including the two type I and the two type II activin receptors, designated ActR-I(A), ActR-IB, ActR-II(A), and ActR-IIB, have been cloned from mammals. Through alternative mRNA splicing, multiple ActR-IIB isoforms can also be generated, adding to the complexity of the activin receptor system. Different activin isoforms bind with different high-affinities to the various type II isoforms. Type I activin receptors do not bind directly to activin but will associate with the type II receptor-activin complex and initiate signal transduction. Besides the activin isoforms, ActR-II will also bind inhibin, BMP-2 and BMP-7 with lower affinities. ActR-I can also bind and form signaling complexes with the BMP-2/7-bound BMPR-II. Activin type I receptors are highly conserved. Human, mouse and bovine type IA activin receptors share greater than 98% amino acid sequence homology. Recombinant soluble activin type I receptor does not bind activin.

References
  1. Attisano, L. et al. (1996) Mol. and Cell. Biol. 16:1066.
  2. Woodruff, T.K. (1998) Biochem. Pharmacology 55:953.
Long Name
Activin Receptor IA
Entrez Gene IDs
90 (Human); 11477 (Mouse)
Alternate Names
activin A receptor, type I; Activin RIA; ActivinRIA; ACTRI; ACVR1; ACVR1A; ACVRLK2; ALK2; ALK-2; FOP; SKR1; TSRI

Product Datasheets

You must select a language.

x
Product Datasheet
COA
SDS

Citations for Human Activin RIA/ALK-2 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
Filter your results:

Filter by:

  1. Clonal hematopoiesis with JAK2V617F promotes pulmonary hypertension with ALK1 upregulation in lung neutrophils
    Authors: Y Kimishima, T Misaka, T Yokokawa, K Wada, K Ueda, K Sugimoto, K Minakawa, K Nakazato, T Ishida, M Oshima, S Koide, K Shide, K Shimoda, A Iwama, K Ikeda, Y Takeishi
    Nature Communications, 2021-10-26;12(1):6177.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Metalloprotease-Dependent Attenuation of BMP Signaling Restricts Cardiac Neural Crest Cell Fate
    Authors: HN Arai, F Sato, T Yamamoto, K Woltjen, H Kiyonari, Y Yoshimoto, C Shukunami, H Akiyama, R Kist, A Sehara-Fuj
    Cell Rep, 2019-10-15;29(3):603-616.e5.
    Species: Human, Mouse
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, IHC-Fr
  3. Bone morphogenetic proteins inhibit CD40L/IL-21-induced Ig production in human B cells: differential effects of BMP-6 and BMP-7.
    Authors: Huse K, Bakkebo M, Oksvold MP, Forfang L, Hilden VI, Stokke T, Smeland EB, Myklebust JH
    Eur. J. Immunol., 2011-10-18;41(11):3135-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Fibroblasts derive from hepatocytes in liver fibrosis via epithelial to mesenchymal transition.
    Authors: Zeisberg M, Yang C, Martino M, Duncan MB, Rieder F, Tanjore H, Kalluri R
    J. Biol. Chem., 2007-06-11;282(32):23337-47.
    Species: Mouse
    Sample Types: Whole Tissue
    Applications: IHC-P

FAQs

No product specific FAQs exist for this product, however you may

View all Antibody FAQs
Loading...

Reviews for Human Activin RIA/ALK-2 Antibody

Average Rating: 3 (Based on 1 Review)

5 Star
0%
4 Star
0%
3 Star
100%
2 Star
0%
1 Star
0%

Have you used Human Activin RIA/ALK-2 Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥2500 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:


Human Activin RIA/ALK-2 Antibody
By Molly Kulikauskas on 08/27/2018
Application: WB Sample Tested: HAEC human aortic endothelial cells,HEK293T human embryonic kidney cell line,HUVEC human umbilical vein endothelial cells Species: Human

10ug of protein collected from untreated HEK, HUVEC, and HAEC cells was loaded into each well.

Blocked in Prometheus OneBlock Western-CL Blocking Buffer for 1hr @ room temp

Primary antibody: Anti-ALK2 MAB637, mouse, RnD 1:500 overnight @ 4C

Secondary antibody: goat-α-mouse HRP (Thermo) 1:10,000 1 hr @ room temp

Substrate: Luminata Forte Western HRP Substrate Millipore 2 min

Imaging: 300 sec exposure