Human ADAM12 DuoSet ELISA

Name: Human ADAM12 DuoSet ELISA
Brand: R&D Systems
SKU: DY4416-05
Rating: 4 (1 reviews)

Catalog #: DY4416-05
4 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
DY4416-05

Ancillary Products Available

DY008B: DuoSet ELISA Ancillary Reagent Kit 2

DY999B: Substrate Reagent Pack

DY999: Substrate Reagent Pack

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All ADAM12 ELISAs

Product Details

Citations (4)

FAQs

Supplemental Products

Reviews (1)

Summary Product Features Kit Content Other Reagents Required Data Examples Product Datasheets Preparation and Storage Background

Human ADAM12 DuoSet ELISA Summary

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Sample Volume Required

100 µL

Assay Range

125.0 - 8,000 pg/mL

Sufficient Materials

For five 96-well plates*

Specificity

Please see the product datasheet

* Provided that the recommended microplates, buffers, diluents, substrates and solutions are used, and the assay is run as summarized in the Assay Procedure provided.

This DuoSet ELISA Development kit contains the basic components required for the development of sandwich ELISAs to measure natural and recombinant human ADAM12. The suggested diluent is suitable for the analysis of most cell culture supernate samples. Diluents for complex matrices, such as serum and plasma, should be evaluated prior to use in this DuoSet ELISA.

Product Features

Optimized capture and detection antibody pairings with recommended concentrations save lengthy development time
Development protocols are provided to guide further assay optimization
Assay can be customized to your specific needs
Economical alternative to complete kits

Kit Content

Capture Antibody
Detection Antibody
Recombinant Standard
Streptavidin conjugated to horseradish-peroxidase (Streptavidin-HRP)

Other Reagents Required

PBS: (Catalog # DY006), or 137 mM NaCl, 2.7 mM KCl, 8.1 mM Na₂HPO₄, 1.5 mM KH₂PO₄, pH 7.2 - 7.4, 0.2 µm filtered

Wash Buffer: (Catalog # WA126), or equivalent

Reagent Diluent*

Blocking Buffer*

Substrate Solution: 1:1 mixture of Color Reagent A (H₂O₂) and Color Reagent B (Tetramethylbenzidine) (Catalog # DY999)

Stop Solution: 2 N H₂SO₄ (Catalog # DY994)

Microplates: R&D Systems (Catalog # DY990), or equivalent

Plate Sealers: ELISA Plate Sealers (Catalog # DY992), or equivalent

*For the Reagent Diluent and Blocking Buffer recommended for a specific DuoSet ELISA Development Kit, please see the product .

Scientific Data

N/A

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Human ADAM12 ELISA Standard Curve

Product Datasheets

Product Datasheet

COA

SDS

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: ADAM12

Members of the ADAM or MDC (Metalloprotease, Disintegrin, Cysteine-rich) family contain pro, metalloprotease-like, disintegrin-like, cysteine-rich, transmembrane and cytoplasmic domains. They play a fundamental role in diverse processes such as asthma, development, angiogenesis and cancer through their activities in cell adhesion/fusion, membrane protein shedding, and signal transduction. Over 30 members have been identified and about half of them are active metalloproteases such as ADAM8, 9, 10, 12 and 17/TACE.

Long Name:

A Disintegrin and Metalloprotease-like Domain 12

Entrez Gene IDs:

8038 (Human)

Alternate Names:

a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-

Citations for Human ADAM12 DuoSet ELISA

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Pro-cancerogenic effects of spontaneous and drug-induced senescence of ovarian cancer cells in vitro and in vivo: a comparative analysis
Authors: S Rutecki, P Szulc, M Paku?a, P Uruski, A Radziemski, E Naumowicz, R Moszy?ski, A Tykarski, J Miku?a-Pie, K Ksi??ek
Journal of ovarian research, 2022-07-26;15(1):87.
Species: Human
Sample Types: Cell Culture Supernates
Serum-based measurements of stromal activation through ADAM12 associate with poor prognosis in colorectal cancer
Authors: S Ten Hoorn, C Waasdorp, MGH van Oijen, H Damhofer, A Trinh, L Zhao, LJH Smits, S Bootsma, GW van Pelt, WE Mesker, L Mol, KKH Goey, M Koopman, JP Medema, JB Tuynman, I Zlobec, CJA Punt, L Vermeulen, MF Bijlsma
BMC Cancer, 2022-04-12;22(1):394.
Species: Human
Sample Types: Serum
Stromal-derived interleukin 6 drives epithelial-to-mesenchymal transition and therapy resistance in esophageal adenocarcinoma
Authors: EA Ebbing, AP van der Za, A Steins, A Creemers, S Hermsen, R Rentenaar, M Klein, C Waasdorp, GKJ Hooijer, SL Meijer, KK Krishnadat, CJA Punt, MI van Berge, SS Gisbertz, OM van Delden, MCCM Hulshof, JP Medema, HWM van Laarho, MF Bijlsma
Proc. Natl. Acad. Sci. U.S.A., 2019-01-22;0(0):.
Species: Human
Sample Types: Serum
ADAM12 is a circulating marker for stromal activation in pancreatic cancer and predicts response to chemotherapy
Authors: VL Veenstra, H Damhofer, C Waasdorp, LB van Rijsse, MJ van de Vij, F Dijk, HW Wilmink, MG Besselink, OR Busch, DK Chang, PJ Bailey, AV Biankin, HM Kocher, JP Medema, JS Li, R Jiang, DW Pierce, HWM van Laarho, MF Bijlsma
Oncogenesis, 2018-11-16;7(11):87.
Species: Human
Sample Types: Serum