Human ADAM15 Ectodomain Biotinylated Antibody

Catalog #: BAF935 Datasheet / COA / SDS

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BAF935

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Human ADAM15 Ectodomain Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM15 in Western blots. In Western blots, approximately 5% cross‑reactivity with recombinant mouse (rm) ADAM15 is observed and less than 1% cross‑reactivity with rmADAM9 and recombinant human ADAM8 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human ADAM15
Met1-Ser693
Accession # Q13444

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human ADAM15 Western Blot Standard (Catalog # WBC027)

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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ADAM15 in Human Prostate. ADAM15 was detected in immersion fixed paraffin-embedded sections of human prostate array using Goat Anti-Human ADAM15 Ectodomain Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF935) at 25 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). Lower panel shows a lack of labeling if primary antibodies are omitted and tissue is stained only with secondary antibody followed by incubation with detection reagents. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ADAM15

ADAM15 belongs to a family of transmembrane proteins that contain disintegrin and metalloprotease domains. Members of this family have been implicated in cell adhesion via integrin binding and shedding of cell surface molecules.

Long Name

A Disintegrin and Metalloprotease-like Domain 15

Entrez Gene IDs

8751 (Human); 11490 (Mouse)

Alternate Names

ADAM 15; ADAM metallopeptidase domain 15; ADAM15; disintegrin-like, and cysteine-rich protein 15; EC 3.4.24; MDC15; MDC-15; Metargidin