Human AgRP/ART Antibody

Catalog #: MAB8311 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB8311
MAB8311-SP

All AgRP/ART Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AgRP/ART Antibody Summary

Species Reactivity

Human

Specificity

Detects human AgRP in direct ELISAs and Western blots. In direct ELISAs, this antibody shows approximately 10% cross-reactivity with recombinant mouse AgRP.

Source

Monoclonal Mouse IgG_2B Clone # 82806

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP
Ala21-Thr132
Accession # O00253

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human AgRP/ART (Catalog # 704-AG)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

AgRP, also known as the protein product of the Agouti related transcript (ART), functions as an antagonist of the melanocortin (MC-3 and MC-4) receptors, both of which are implicated in obesity. AgRP levels are significantly elevated in the serum of obese mice and people.

Long Name

Agouti-related Protein

Entrez Gene IDs

181 (Human); 11604 (Mouse)

Alternate Names

agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2