Human AgRP/ART Antibody

Catalog # Availability Size / Price Qty
AF704
AF704-SP
AgRP/ART in Human Brain.
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Product Details
Citations (1)
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Human AgRP/ART Antibody Summary

Species Reactivity
Human
Specificity
Detects human AgRP/ART in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse AgRP/ART is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human AgRP/ART
Ala21-Thr132
Accession # O00253
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human AgRP/ART (Catalog # 704-AG)
Immunohistochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry AgRP/ART antibody in Human Brain by Immunohistochemistry (IHC-P). View Larger

AgRP/ART in Human Brain. AgRP/ART was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human AgRP/ART Antigen Affinity-purified Polyclonal Antibody (Catalog # AF704) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown ; Catalog # CTS008) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AgRP/ART

Agouti-Related Protein (AgRP), the protein product of the Agouti-Related Transcript (ART), is a neuropeptide that regulates energy metabolism and the development of obesity by antagonizing alpha -melanocyte stimulating hormone ( alpha -MSH) on MC-3 and MC-4 receptors (1 - 4). AgRP is predominantly expressed in the hypothalamus and adrenal medulla (5). Mature human AgRP is a 112 amino acid (aa) peptide; its C-terminal portion contains ten conserved cysteines that form five disulfide bonds (5, 6). Human AgRP shares 82% and 72% aa sequence identity with mouse and rat AgRP, respectively. It shares 32% aa sequence identity with Agouti. As in the case of Agouti, the C-terminal cysteine-rich region is sufficient for biological activity (7). AgRP is 100 times more potent than Agouti in antagonizing MC-3 and MC-4 receptors (8). AgRP also induces the beta -arrestin dependent endocytosis of MC-3 and MC-4 (9). Hypothalamic expression of AgRP is upregulated in obesity and diabetes (5, 10), and chronic AgRP administration increases food intake and weight gain in rats (11). Genetically-linked polymorphisms of AgRP in humans are associated with susceptibility to anorexia nervosa (12, 13). In addition, AgRP inhibits the ACTH-induced synthesis of steroid hormones in a mechanism that does not involve melanocortin receptors (14).

References
References
  1. Martin, N.M. et al. (2006) Peptides 27:333.
  2. Fan, W. et al. (1997) Nature 385:165.
  3. Ollmann, M.M. et al. (1997) Science 278:135.
  4. Arora, S. and Anubhuti (2006) Neuropeptides 40:375.
  5. Shutter, J.R. et al. (1997) Genes Develop. 11:593.
  6. Kiefer, L.L. et al. (1998) Biochemistry 37:991.
  7. Jackson, P.J. et al. (2002) Biochemistry 41:7565.
  8. Fong, T.M. et al. (1997) Biochem. Biophys. Res. Commun. 237:629.
  9. Breit, A. et al. (2006) J. Biol. Chem. 281:37447.
  10. Katsuki, A. et al. (2001) J. Clin. Endocrinol. Metab. 86:1921.
  11. Small, C.K. et al. (2001) Diabetes 50:248.
  12. Vink, T. et al. (2001) Mol. Psychiatry 6:325.
  13. Dardennes, R.M. et al. (2007) Psychoneuroendocrinology 32:106.
  14. Doghman, M. et al. (2007) Mol. Cell. Endocrinol. 265 - 266:108.

Long Name
Agouti-related Protein
Entrez Gene IDs
181 (Human); 11604 (Mouse)
Alternate Names
agouti related protein homolog (mouse); agouti-related protein; AgRP; Agrt; AGRTMGC118963; ART; ARTagouti (mouse) related protein; ASIP2

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Citation for Human AgRP/ART Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Ectopic ACTH Syndrome Emerging 5 Years after the Diagnosis of Neuroendocrine Tumor
    Authors: Minghao Liu, Diane Hamele-Bena, John Ausiello, Gabrielle Page-Wilson
    Case Reports in Endocrinology

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