Human ALK-1 Antibody

Catalog #: MAB370
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB370-SP
MAB370-500
MAB370-100

Detection of Human ALK-1 by Western Blot.

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All ALK-1 Antibodies

Product Details

Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human ALK-1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ALK-1 in direct ELISAs and Western blots. Shows less than 1% cross‑reactivity with recombinant mouse ALK-1, recombinant human (rh) Activin RIA, or rhActivin RIB.

Source

Monoclonal Mouse IgG_2A Clone # 117720

Purification

Protein A or G purified from ascites

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ALK‑1
Asp22-Gln118
Accession # P37023

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from ascites

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

40 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human ALK-1 by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line and human placenta tissue. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human ALK-1 Monoclonal Antibody (Catalog # MAB370) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for ALK-1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human ALK‑1 by Simple Western^TM. Simple Western lane view shows lysates of human placenta tissue and human lung tissue, loaded at 0.2 mg/mL. A specific band was detected for ALK‑1 at approximately 63 kDa (as indicated) using 40 µg/mL of Mouse Anti-Human ALK‑1 Monoclonal Antibody (Catalog # MAB370). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: ALK-1

Transforming Growth Factor beta (TGF-beta ) superfamily ligands exert their biological activities via binding to heteromeric receptor complexes of two types (I and II) of serine/threonine kinases. Type II receptors are constitutively active kinases that phosphorylate type I receptors upon ligand binding. In turn, activated type I kinases phosphorylate downstream signaling molecules including the various smads. Transmembrane proteoglycans, including the type III receptor (betaglycan) and endoglin, can bind and present some of the TGF-beta superfamily ligands to type I and II receptor complexes and enhance their cellular responses. Seven type I receptors (also termed activin receptor-like kinase (ALK)) and five type II receptors have been isolated from mammals. ALK-2, -3, -4, -5, and -6 are also known as Activin R1A, BMPR-1A, Activin R1B, TGF-beta R1, and BMPR-1B, respectively, reflecting their ligand preferences. Evidence suggests that TGF-beta 1, TGF-beta 3 and an unknown ligand present in serum can activate chimeric ALK-1. ALK-1 shares with other type I receptors a cysteine-rich domain with conserved cysteine spacing in the extracellular region, and a glycine- and serine-rich domain (the GS domain) preceding the kinase domain. ALK-1 is expressed highly in endothelial cells and other highly vascularized tissues. The expression patterns of ALK-1 parallels that of endoglin. Mutations in ALK-1 as well as in endoglin are associated with hereditary hemorrhagic telangiectasia (HHT), suggesting a critical role for ALK-1 in the control of blood vessel development or repair. Human and mouse ALK-1 share approximately 71% amino acid sequence identity in their extracellular regions.

References

ten Dijke, P. et al. (1993) Oncogene 8:2879.
ten Dijke, P. et al. (1994) Science 264:101.
Lux, A. et al. (1999) J. Biol. Chem. 274:9984.

Long Name

Activin Receptor-like Kinase 1

Entrez Gene IDs

94 (Human); 11482 (Mouse)

Alternate Names

activin A receptor type II-like 1; activin A receptor, type II-like kinase 1; Activin receptor-like kinase 1; ACVRL1; ACVRLK1ORW2; ALK1; ALK-1; ALK1TGF-B superfamily receptor type I; EC 2.7.11; HHT; HHT2EC 2.7.11.30; serine/threonine-protein kinase receptor R3; SKR3; TSR-I

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Related Research Areas

Citations for Human ALK-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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ALK1 opposes ALK5/Smad3 signaling and expression of extracellular matrix components in human chondrocytes.
Authors: Finnson KW, Parker WL, ten Dijke P, Thorikay M, Philip A
J. Bone Miner. Res., 2008-06-01;23(6):896-906.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Antitumor activity of ALK1 in pancreatic carcinoma cells.
Authors: Ungefroren H, Schniewind B, Groth S, Chen WB, Muerkoster SS, Kalthoff H, Fandrich F
Int. J. Cancer, 2007-04-15;120(8):1641-51.
Species: Human
Sample Types: Whole Cells
Applications: Western Blot
Therapeutic action of tranexamic acid in hereditary haemorrhagic telangiectasia (HHT): regulation of ALK-1/endoglin pathway in endothelial cells.
Authors: Fernandez-L A, Garrido-Martin EM, Sanz-Rodriguez F, Ramirez JR, Morales-Angulo C, Zarrabeitia R, Perez-Molino A, Bernabeu C, Botella LM
Thromb. Haemost., 2007-02-01;97(2):254-62.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry