Human alpha 2-Macroglobulin Antibody Summary
Applications
This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human alpha 2-Macroglobulin DuoSet ELISA Kit (Catalog # DY1938) for convenient development of a sandwich ELISA.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
![](https://resources.rndsystems.com/images/datasheets/antibody/mab19381_human-alpha-2-macroglobulin-mab-clone-257303-elisa-16122022123219.png)
Human alpha 2‑Macroglobulin ELISA Standard Curve. Recombinant Human alpha 2‑Macroglobulin protein was serially diluted 2-fold and captured by Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB19381) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human alpha 2‑Macroglobulin Biotinylated Antigen Affinity-purified Polyclonal Antibody (Catalog # BAF1938) was incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Product Datasheets
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