Human alpha-Synuclein Antibody

Name: Human alpha-Synuclein Antibody
Brand: R&D Systems
SKU: MAB13383
Rating: 5 (1 reviews)

Catalog #: MAB13383 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB13383-100
MAB13383-SP

Detection of Human alpha -Synuclein by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human alpha-Synuclein Antibody Summary

Species Reactivity

Human

Specificity

Detects human alpha -Synuclein in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 970114

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human alpha -Synuclein
Met1-Ala140
Accession # P37840

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

5 µg/mL

See below

Immunohistochemistry

5-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human alpha -Synuclein by Western Blot. Western blot shows lysates of human brain (motor cortex and cerebellum) tissue. PVDF membrane was probed with 5 µg/mL of Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a-Synuclein at approximately 17 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Immunohistochemistry

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alpha -Synuclein in Human Brain. a-Synuclein was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Mouse Anti-Human a-Synuclein Monoclonal Antibody (Catalog # MAB13383) at 5 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to synaptic boutons. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: alpha-Synuclein

alpha -Synuclein is a cytoplasmic protein that is predominantly expressed in the central nervous system. It is a precursor of a minor peptide found in amyloid deposition that is a hallmark of Alzheimer's disease. Defects in alpha -Synuclein are associated with familial Parkinson's disease.

Entrez Gene IDs

6622 (Human); 20617 (Mouse); 29219 (Rat); 641350 (Porcine); 282857 (Bovine)

Alternate Names

alpha-Synuclein; I+/--synuclein; MGC110988; NACP; non A-beta component of AD amyloid; Non-A beta component of AD amyloid; Non-A4 component of amyloid precursor; PARK1; PARK4; PD1; SNCA; synuclein alpha-140; synuclein, alpha (non A4 component of amyloid precursor); Synuclein-alpha; truncated alpha synuclein