Human APH1A Antibody

Catalog #: MAB6908 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB6908
MAB6908-SP

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human APH1A Antibody Summary

Species Reactivity

Human

Specificity

Detects human APH1A in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 665404

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human APH1A synthetic peptide
SSFERFEIFPKHVTDRSDARLQY
Accession # Q96BI3

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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APH1A in Human Brain. APH1A was detected in immersion fixed paraffin-embedded sections of human Alzheimer's brain using Mouse Anti-Human APH1A Monoclonal Antibody (Catalog # MAB6908) at 15 µg/mL overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to neurons. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: APH1A

Anterior pharynx-defective 1 (APH1A) is a widely expressed 36 kDa 7-transmembrane protein found in the endoplasmic reticulum and Golgi. APH1A is a component of the gamma secretase proteolytic complex which cleaves multiple substrates within their transmembrane domains. This activity liberates the intracellular domains of ErbB4, Neuregulin-1, and Notch receptors in addition to the A-beta peptide from the amyloid precursor protein (APP). Alternate splicing generates a short isoform of human APH1A that lacks 18 amino acids at the C-terminus. Within aa 58-69, human APH1A shares 100% aa sequence identity with mouse and rat APH1A.

Long Name

gamma-Secretase Subunit Anterior Pharynx Defective 1 Homolog A

Entrez Gene IDs

51107 (Human); 100039623 (Mouse)

Alternate Names

6530402N02Rik; anterior pharynx defective 1 homolog A (C. elegans); APH-1; APH1A; APH-1A; aph-1alpha; CGI-78; gamma-secretase subunit APH-1A; Presenilin-stabilization factor; PSF