Human Apolipoprotein A-I/ApoA1 Antibody

Catalog #: AF3664
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF3664
AF3664-SP

Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein A-I/ApoA1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human ApoA1 in direct ELISAs and Western blots. In Western blots, less than 1% cross‑reactivity with recombinant human (rh) ApoA2, rhApoB100, and rhApoC2 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Human Plasma ApoA1 and E. coli-derived recombinant human ApoA1
Asp25-Gln267
Accession # P02647

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.5 µg/mL

See below

Simple Western

5 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Apolipoprotein A‑I/ApoA1 by Western Blot. Western blot shows human plasma. PVDF membrane was probed with 0.5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Simple Western

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Detection of Human Apolipoprotein A‑I/ApoA1 by Simple Western^TM. Simple Western lane view shows lysates of human serum, loaded at 1:5000. A specific band was detected for Apolipoprotein A-I/ApoA1 at approximately 32 kDa (as indicated) using 5 µg/mL of Goat Anti-Human Apolipoprotein A-I/ApoA1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3664) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein A-I/ApoA1

ApoA1 is a major protein component of serum high-density lipoprotein (HDL) and is produced by the liver and small intestine. It is involved in reverse cholesterol transport from tissues to the liver. ApoA1 is synthesized as a 267 amino acid (aa) precursor from which a signal peptide and short propeptide are removed. Mature human ApoA1 shares 65% and 61% aa sequence identity with mouse and rat ApoA1, respectively.

Entrez Gene IDs

335 (Human); 11806 (Mouse); 25081 (Rat)

Alternate Names

Alp-1; APOA1; Apo-AI; apoA-I; Apolipoprotein A1; Apolipoprotein AI; Apolipoprotein A-I; Brp-14; Ltw-1; Lvtw-1; MGC117399; Sep-1; Sep-2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human Apolipoprotein A-I/ApoA1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Mitochondrial Electron Transport Chain Protein Abnormalities Detected in Plasma Extracellular Vesicles in Alzheimer's Disease
Authors: PJ Yao, E Eren, EJ Goetzl, D Kapogianni
Biomedicines, 2021-10-31;9(11):.
Species: Human
Sample Types: Extracellular
Applications: Western Blot
Serum amyloid A is a soluble pattern recognition receptor that drives type 2 immunity
Authors: U Smole, N Gour, J Phelan, G Hofer, C Köhler, B Kratzer, PA Tauber, X Xiao, N Yao, J Dvorak, L Caraballo, L Puerta, S Rosskopf, J Chakir, E Malle, AP Lane, WF Pickl, S Lajoie, M Wills-Karp
Nat. Immunol., 2020-06-22;21(7):756-765.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Immunoprecipitation
Adenosine leakage from perforin-burst extracellular vesicles inhibits perforin secretion by cytotoxic T-lymphocytes
Authors: H Tadokoro, A Hirayama, R Kudo, M Hasebe, Y Yoshioka, J Matsuzaki, Y Yamamoto, M Sugimoto, T Soga, T Ochiya
PLoS ONE, 2020-04-10;15(4):e0231430.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot
Aspirin hydrolysis in plasma is a variable function of butyrylcholinesterase and platelet-activating factor acetylhydrolase 1b2 (PAFAH1b2).
Authors: Zhou G, Marathe G, Hartiala J, Hazen S, Allayee H, Tang W, McIntyre T
J Biol Chem, 2013-03-18;288(17):11940-8.
Species: Human
Sample Types: Plasma
Applications: Western Blot