Human Apolipoprotein H/ApoH Antibody

Catalog #: MAB5087
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB5087
MAB5087-SP

Detection of Human Apolipoprotein H/ApoH by Western Blot.

2 Images

Product Details

Citations (1)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Apolipoprotein H/ApoH Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human Apolipoprotein H/ApoH in Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 517038

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Apolipoprotein H/ApoH
Gly20-Cys345
Accession # P02749

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Simple Western

10 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

View Larger

Detection of Human Apolipoprotein H/ApoH by Western Blot. Western blot shows lysates of human liver tissue and an enriched HDL/LDL fraction of human plasma obtained from ultracentrifugation. PVDF membrane was probed with 1 µg/mL of Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Apolipoprotein H/ApoH at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Simple Western

View Larger

Detection of Human Apolipoprotein H/ApoH by Simple Western^TM. Simple Western lane view shows human plasma, loaded at 0.2 mg/mL. A specific band was detected for Apolipoprotein H/ApoH at approximately 67 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human Apolipoprotein H/ApoH Monoclonal Antibody (Catalog # MAB5087). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Apolipoprotein H/ApoH

Apolipoprotein H (ApoH also known as beta 2-Glycoprotein 1/ beta 2-GPI) is a 50 kDa secreted monomeric glycoprotein member of the complement control superfamily of molecules. It is produced by hepatocytes and is bound to HDL particles. Circulating ApoH blocks the intrinsic clotting pathway and binds to exposed phospholipids on apoptotic cells. Mature human ApoH contains four Sushi repeats (aa 21‑260) and one C-terminal kringle domain (aa 261‑345) that binds heparin and phospholipids. At least two isoforms exist. One shows a 31 aa substitution for aa 140‑345, while a second represents a bioactive plasmin cleavage product that removes the C-terminal nine amino acids. Mature human ApoH shares 76% aa identity with mouse ApoH.

Entrez Gene IDs

350 (Human); 11818 (Mouse); 287774 (Rat)

Alternate Names

Activated protein C-binding protein; Anticardiolipin cofactor; APC inhibitor; APOH; apo-H; apolipoprotein H (beta-2-glycoprotein I); Apolipoprotein H; B2G1; B2GP1; B2GPI; Beta; beta(2)GPI; beta-2-glycoprotein 1; Beta-2-glycoprotein I; BG

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Apolipoprotein H/ApoH Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

Streptococcal M1 protein constructs a pathological host fibrinogen network.
Authors: Macheboeuf P, Buffalo C, Fu CY, Zinkernagel AS, Cole JN, Johnson JE, Nizet V, Ghosh P
Nature, 2011-04-07;472(7341):64-8.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot