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Human BAFF/BLyS/TNFSF13B Antibody

Catalog #: AF124
11 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF124
AF124-SP
Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody.
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Citations (11)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BAFF/BLyS/TNFSF13B Antibody Summary

Species Reactivity
Human
Specificity
Detects human BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, less than 15% cross-reactivity with recombinant murine BAFF and less than 1% cross‑reactivity with recombinant human (rh) APRIL is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BAFF/BLyS/TNFSF13B
Ala134-Leu285
Accession # Q9Y275
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.60 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF)
Immunohistochemistry
5-15 µg/mL
See below
Neutralization
Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 3-12 ng/mL in the presence of 5 ng/mL Recombinant Human BAFF/BLyS/TNFSF13B and 1 µg/mL Goat F(ab')2 Anti-mouse IgM.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody. View Larger

Cell Proliferation Induced By BAFF/BLyS/TNFSF13B and Neutralization by Human BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM (1 µg/mL), Recombinant Human BAFF/BLyS/TNFSF13B (Catalog # 2149-BF) stimulates proliferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Human BAFF/BLyS/TNFSF13B (5 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124). The ND50 is typically 3-12 ng/mL.

Immunohistochemistry BAFF/BLyS/TNFSF13B antibody in Human Spleen by Immunohistochemistry (IHC-P). View Larger

BAFF/BLyS/TNFSF13B in Human Spleen. BAFF/BLyS/TNFSF13B was detected in formalin fixed paraffin-embedded sections of human spleen using 15 µg/mL Goat Anti-Human BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF124) overnight at 4 °C. Tissue was stained with the Anti-Goat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS008) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, and THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Human BAFF is a 285 amino acid (aa) protein consisting of a 218 aa extracellular domain, a 21 aa transmembrane region and a 46 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Human BAFF may be shed from the cell surface by proteolytic cleavage between R133 and Ala134 to yield a soluble form of the protein that is detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressd in B cells. BAFF and APRIL can form active heteromers that bind to TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is up-regulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

References
  1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
  2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
  3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145.
  4. Liu, Y. et al. (2002) Cell 108:383.
  5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
  6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
  7. Thompson, J.S. et al. (2001) Science 293:2108.
  8. Ng, L.G. et al. (2004) J. Immunol. 173:807.
  9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
  10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
  11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
Long Name
B cell Activating Factor
Entrez Gene IDs
10673 (Human); 24099 (Mouse); 498666 (Rat); 102123214 (Cynomolgus Monkey)
Alternate Names
ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4

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Citations for Human BAFF/BLyS/TNFSF13B Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

11 Citations: Showing 1 - 10
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  1. BDNF belongs to the nurse-like cell secretome and supports survival of B chronic lymphocytic leukemia cells
    Authors: H Talbot, S Saada, E Barthout, PF Gallet, N Gachard, J Abraham, A Jaccard, D Troutaud, F Lalloué, T Naves, AL Fauchais, MO Jauberteau
    Sci Rep, 2020-07-28;10(1):12572.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. BAFF is involved in the pathogenesis of IgA nephropathy by activating the TRAF6/NF?&kappaB signaling pathway in glomerular mesangial cells
    Authors: Y Cao, G Lu, X Chen, X Chen, N Guo, W Li
    Mol Med Rep, 2019-12-06;21(2):795-805.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  3. IFN-? stimulates CpG-induced IL-10 production in B cells via p38 and JNK signalling pathways
    Authors: M Imbrechts, K De Samblan, K Fierens, E Brisse, J Vandenhaut, T Mitera, C Libert, I Smets, A Goris, C Wouters, P Matthys
    Eur. J. Immunol., 2018-08-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Plasmacytoid dendritic cells and RNA-containing immune complexes drive expansion of peripheral B cell subsets with an SLE-like phenotype
    Authors: O Berggren, N Hagberg, A Alexsson, G Weber, L Rönnblom, ML Eloranta
    PLoS ONE, 2017-08-28;12(8):e0183946.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  5. Serum B cell-activating factor (BAFF) level in connective tissue disease associated interstitial lung disease.
    Authors: Hamada T, Samukawa T, Kumamoto T, Hatanaka K, Tsukuya G, Yamamoto M, Machida K, Watanabe M, Mizuno K, Higashimoto I, Inoue Y, Inoue H
    BMC Pulm Med, 2015-09-30;15(0):110.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  6. The B Cell-Stimulatory Cytokines BLyS and APRIL Are Elevated in Human Periodontitis and Are Required for B Cell-Dependent Bone Loss in Experimental Murine Periodontitis.
    Authors: Abe T, AlSarhan M, Benakanakere M, Maekawa T, Kinane D, Cancro M, Korostoff J, Hajishengallis G
    J Immunol, 2015-07-06;195(4):1427-35.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  7. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2015-07-03;30(1):163-72.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  8. BlyS is up-regulated by hypoxia and promotes migration of human breast cancer cells.
    Authors: Zhu J, Sun L, Lin S
    J. Exp. Clin. Cancer Res., 2012-03-31;31(0):31.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: ICC, Western Blot
  9. Unusual presentation of multiple pathologic bone fractures in a patient with gastric mucosa-associated lymphoid tissue lymphoma.
    Authors: Kuo SH, Yen RF, Lin CW, Chen LT, Tien HF, Cheng AL
    Ann. Hematol., 2009-08-18;89(4):431-6.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  10. Essential role of antigen-presenting cell-derived BAFF for antibody responses.
    Authors: Bergamin F, Vincent IE, Summerfield A, McCullough KC
    Eur. J. Immunol., 2007-11-01;37(11):3122-30.
    Species: Porcine
    Sample Types: Whole Cells
    Applications: ICC
  11. B-lymphocyte stimulator (BLyS) stimulates immunoglobulin production and malignant B-cell growth in Waldenstrom macroglobulinemia.
    Authors: Elsawa SF, Novak AJ, Grote DM, Ziesmer SC, Witzig TE, Kyle RA, Dillon SR, Harder B, Gross JA, Ansell SM
    Blood, 2005-11-22;107(7):2882-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human BAFF/BLyS/TNFSF13B Antibody
By Anonymous on 02/18/2022
Application: IHC Sample Tested: Spleen tissue Species: Human

Heat-retrieval in high-pH buffer
primary Ab 10ug/mL for 60min
ImmPRESS polymer HRP and DAB

Immunohistochemistry Human BAFF/BLyS/TNFSF13B Antibody AF124