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Human BCAM Antibody

Catalog #: AF148
4 Citations Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF148
AF148-SP
Detection of Human BCAM by Western Blot.
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Citations (4)
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BCAM Antibody Summary

Species Reactivity
Human
Specificity
Detects human BCAM in direct ELISAs and Western blots. In these formats, approximately 2% cross-reactivity with recombinant human (rh) ALCAM is observed and less than 1% cross-reactivity with rhPECAM, rhEpCAM, rhICAM‑1, rhICAM‑2, rhICAM‑3, and rhVCAM‑1 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
Mouse myeloma cell line NS0-derived recombinant human BCAM
Glu32-Ala547
Accession # CAA58449
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Adhesion Blockade
The adhesion of TE-85 human osteogenic sarcoma cells (5 x 104 cells/well) to immobilized Recombinant Human BCAM Fc Chimera (Catalog # 148-BC, 10 µg/mL, 100 µL/well) was maximally inhibited (80‑100%) by 25 µg/mL of the antibody.
 

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human BCAM antibody by Western Blot. View Larger

Detection of Human BCAM by Western Blot. Western blot shows lysates of LNCaP human prostate cancer cell line, human prostate tissue, human kidney tissue, and human thyroid tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human BCAM Antigen Affinity-purified Polyclonal Antibody (Catalog # AF148) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for BCAM at approximately 70-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BCAM

Basal-Cell Adhesion Molecule (BCAM) and Lutheran blood group glycoprotein (LU) are two alternatively spliced variants of a single immunoglobulin superfamily (IgSF) protein that differ in the length of their cytoplasmic tails. BCAM cDNA encodes a 628 amino acid (aa) residues precursor protein with a putative 31 aa signal peptide, a 597 aa extracellular domain containing three C2 type and two V-type Ig like domains, a 21 aa transmembrane domain, and a 19 aa cytoplasmic domain. Compared to the 40 aa cytoplasmic domain present in LU, the BCAM cytoplasmic tail lacks the putative Src homology 3 (SH3) binding site that may be involved in mediating intracellular signaling. BCAM/LU has wide tissue distribution and is expressed on erythrocytes, the endothelium of blood vessels and on the basal layer of cells in the epithelia. The expression of BCAM/LU in normal tissues is higher in fetal versus adult tissues. BCAM/LU expression is also upregulated in sickle cell disease red blood cells, in activated keratinocytes and following malignant transformation in some cell types in vivo and in vitro. BCAM/LU has been shown to be an adhesion molecule that binds laminin, a basement membrane protein involved in cell differentiation, adhesion, migration and proliferation.

References
  1. Campbell, I.G. et al. (1994) Cancer Research 54:5761.
  2. Parsons, S.F. et al. (1995) Proc. Natl. Acad. Sci. USA 92:5496.
  3. Udani, M. et al. (1998) J. Clin. Invest. 101:2550.
  4. Schon, M. et al. (2000) J. Invest. Dermatol. 115:1047.
Long Name
Basal Cell Adhesion Molecule
Entrez Gene IDs
4059 (Human); 57278 (Mouse)
Alternate Names
antigen identified by monoclonal F8; Auberger B antigen; basal cell adhesion molecule (Lu and Au blood groups); basal cell adhesion molecule (Lutheran blood group); basal cell adhesion molecule; B-CAM cell surface glycoprotein; BCAM; B-cell adhesion molecule; CD239 antigen; CD239; F8/G253 antigen; glycoprotein 95kDa; LUAU; Lutheran antigen; Lutheran blood group (Auberger b antigen included); Lutheran blood group glycoprotein; MSK19

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Citations for Human BCAM Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Glycophorin-C sialylation regulates Lu/BCAM adhesive capacity during erythrocyte aging
    Authors: TRL Klei, DZ de Back, PJ Asif, PJJH Verkuijlen, M Veldthuis, PC Ligthart, J Berghuis, E Clifford, BM Beuger, TK van den Be, R van Zwiete, W El Nemer, R van Brugge
    Blood Adv, 2018-01-03;2(1):14-24.
    Species: Human
    Sample Types: Cell Lysates, Whole Cells
    Applications: Flow Cytometry, Western Blot
  2. Hypoxia enhanced adhesion of red blood cells in microscale flow
    Authors: M Kim, Y Alapan, A Adhikari, JA Little, UA Gurkan
    Microcirculation, 2017-07-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Bioassay
  3. Clonal culturing of human embryonic stem cells on laminin-521/E-cadherin matrix in defined and xeno-free environment.
    Authors: Rodin S, Antonsson L, Niaudet C, Simonson O, Salmela E, Hansson E, Domogatskaya A, Xiao Z, Damdimopoulou P, Sheikhi M, Inzunza J, Nilsson A, Baker D, Kuiper R, Sun Y, Blennow E, Nordenskjold M, Grinnemo K, Kere J, Betsholtz C, Hovatta O, Tryggvason K
    Nat Commun, 2014-01-01;5(0):3195.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  4. Identification and characterization of Lutheran blood group glycoprotein as a new substrate of membrane-type 1 matrix metalloproteinase 1 (MT1-MMP): a systemic whole cell analysis of MT1-MMP-associating proteins in A431 cells.
    Authors: Niiya D, Egawa N, Sakamoto T, Kikkawa Y, Shinkawa T, Isobe T, Koshikawa N, Seiki M
    J. Biol. Chem., 2009-08-10;284(40):27360-9.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot

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