Human BIK Antibody

Catalog #: AF5474 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF5474
AF5474-SP

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Human BIK Antibody Summary

Species Reactivity

Human

Specificity

Detects human BIK in Western Blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BIK
Met1-Gln135
Accession # Q13323

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunohistochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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BIK in Human Lymphoma. BIK was detected in immersion fixed paraffin-embedded sections of human lymphoma tissue using Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) at 10 µg/mL overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Western Blot

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Detection of Human BIK by Western Blot. Western blot shows lysates of Ramos human Burkitt's lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Human BIK Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5474) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BIK at approximately 22 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BIK

Bcl-2-interacting killer (BIK; also known as NBK, BP4 and BIP-1) is a 19 kDa member of the BH3-only subfamily, Bcl-2 family of proteins. It is widely expressed, and serves as an initiator of cell death. It apparently participates in apoptosis by sequestering antiapoptotic cytoplasmic proteins of the Bcl-2 family and in autophagy by regulating cytosolic calcium levels. Human BIK-1 is 160 amino acids (aa) in length. It contains one BH3 domain (aa 57‑74), a “death segment” (aa 121‑134) and a transmembrane domain (aa 136‑156). BIK is likely to embed in both outer mitochondrial and ER membranes. Over amino acids 1‑135, human BIK shares only 45% aa identity with mouse BIK.

Long Name

Bcl-2 Interacting Killer

Entrez Gene IDs

638 (Human)

Alternate Names

Apoptosis inducer NBK; BCL2-interacting killer (apoptosis-inducing); bcl-2-interacting killer; BIK; BIP1; BP4; NBK; NBKapoptosis-inducing NBK