Human BMP-2/BMP-4 Biotinylated Antibody

Catalog #: BAF355
2 Citations Datasheet / COA / SDS

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BAF355

All BMP-2/BMP-4 Antibodies

Product Details

Citations (2)

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BMP-2/BMP-4 Biotinylated Antibody Summary

Species Reactivity

Human

Specificity

Detects human BMP‑2/BMP‑4 in Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human BMP‑2
Ala284-Arg396
Accession # P12643

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.

Label

Biotin

Purity

Antigen Affinity-purified

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human BMP‑2 (Catalog # 355-BM)
Recombinant Human BMP‑4 (Catalog # 314-BP)

Immunocytochemistry

5-15 µg/mL

Immersion fixed rat neonatal dorsal root ganglia cells

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BMP-2/BMP-4

Bone morphogenetic protein 2 (BMP-2) is a member of the BMP subgroup of the TGF-beta superfamily. It plays a dominant role in embryonic dorsal-ventral patterning, organogenesis, limb bud formation, and bone formation and regeneration (1, 2). Human BMP-2 is synthesized as a 396 amino acid (aa) preproprotein that contains a 23 aa signal sequence, a 259 aa prosegment, and a 114 aa mature region (3). Proteolytic removal of the propeptide enables mature BMP-2 to form active disulfide linked homodimers and heterodimers with BMP-7 (2). Mature monomeric BMP-2 is an 18 kDa glycosylated peptide with seven conserved cysteines that form a cystine knot structure (4). Mature human BMP-2 shares 100% aa sequence identity with mouse and rat BMP-2. It shares 85% aa sequence identity with human BMP-4 and less than 51% with other BMPs. BMP-2 signals through heterodimeric complexes composed of a type I receptor (Activin RI, BMPR-IA, or BMPR-IB) and a type II receptor (BMP RII or Activin RIIB) (2, 5). BMP-2 induces chondrocyte proliferation, endochondral bone formation, longitudinal bone growth, and bone and cartilage repair (6, 7). It induces ectopic bone formation or calcification by promoting osteogenic and chondrogenic differentiation in mesenchymal cells, stem cells, and vascular smooth muscle cells (2, 8‑10). BMP-2/BMP-7 heterodimers are significantly more potent than BMP-2 homodimers at inducing bone formation in vivo (11). BMP-2 also promotes the maintenance and repair of colonic epithelium, suppresses neuronal dopamine synthesis and release, induces apoptosis in medulloblastoma cells, and is required for cardiac contractility (12‑15).

References

Kishigami, S. and Y. Mishina (2005) Cytokine Growth Factor Rev. 16:265.
Chen, D. et al. (2004) Growth Factors 22:233.
Wozney, J. et al. (1988) Science 242:1528.
Sun, P.D. and D.R. Davies (1995) Annu. Rev. Biophys. Biomol. Struct. 24:269.
Sebald, W. et al. (2004) Biol. Chem. 385:697.
De Luca, F. et al. (2001) Endocrinology 142:430.
Davidson, E.N.B., et al. (2007) Arthritis Res. Ther. 9:R102.
Ryoo, H.-M. et al. (2006) Gene 366:51.
Kramer, J. et al. (2000) Mech. Dev. 92:193.
Li, X. et al. (2008) Atherosclerosis January 5 epub.
Zhu, W. et al. (2004) J. Bone Miner. Res. 19:2021.
Peiris, D. et al. (2007) Am. J. Physiol. Gastrointest. Liver Physiol. 292:G753.
Kano, Y. et al. (2005) Endocrinology 146:5332.
Hallahan, A.R. et al. (2003) Nat. Med. 9:1033.
Wang, Y.-X. et al. (2007) Cardiovasc. Res. 74:290.

Long Name

Bone Morphogenetic Protein 2/4

Entrez Gene IDs

650 (Human); 12156 (Mouse); 29373 (Rat)

Alternate Names

BDA2; BMP-2; BMP-2/BMP-4; BMP2A; BMP-2A; BMP2ABone morphogenetic protein 2A; bone morphogenetic protein 2

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human BMP-2/BMP-4 Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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PLAP-1/asporin inhibits activation of BMP receptor via its leucine-rich repeat motif.
Authors: Tomoeda M, Yamada S, Shirai H, Ozawa Y, Yanagita M, Murakami S
Biochem. Biophys. Res. Commun., 2008-04-11;371(2):191-6.
Species: Human
Sample Types: Complex Sample Type
Applications: Western Blot
Baalc, a marker of mesoderm and muscle.
Authors: Satoskar AA, Tanner SM, Weinstein M, Qualman SJ, de la Chapelle A
Gene Expr. Patterns, 2005-04-01;5(4):463-73.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC