Human BTNL8 Antibody

Catalog #: MAB9359 Datasheet / COA / SDS

Recombinant Monoclonal Antibody

Catalog #	Availability	Size / Price	Qty
MAB9359-100
MAB9359-SP

Detection of Human BTNL8 by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human BTNL8 Antibody Summary

Species Reactivity

Human

Specificity

Detects human BTNL8 in direct ELISAs and Western blots.

Source

Recombinant Monoclonal Rabbit IgG Clone # 2187B

Purification

Protein A or G purified from cell culture supernatant

Immunogen

Human embryonic kidney cell line HEK-293-derived Human BTNL8
Gln18-Lys238
Accession # Q6UX41-1

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Simple Western

50 µg/mL

See below

Flow Cytometry

0.25 µg/5 x 10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human BTNL8 by Western Blot. Western blot shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with human BTNL8 and eGFP. PVDF membrane was probed with 2 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) followed by HRP-conjugated Anti-Rabbit IgG Secondary Antibody (Catalog # HAF008). A specific band was detected for BTNL8 at approximately 68 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of BTNL8 in HEK293 Human Cell Line Transfected with Human BTNL8 and eGFP by Flow Cytometry. HEK293 human embryonic kidney cell line transfected with human BTNL8 and eGFP was stained with either (A) Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359) or (B) Rabbit IgG control antibody (Catalog # MAB1050) followed by APC-conjugated Goat anti-Rabbit IgG Secondary Antibody (Catalog # F0111). View our protocol for Staining Membrane-associated Proteins.

Simple Western

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Detection of Human BTNL8 by Simple Western^TM. Simple Western lane view shows lysates of Hek293T human embryonic kidney cell line either mock transfected or transfected with with human BTNL8 and eGFP, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 66 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Simple Western

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Detection of Human BTNL8 by Simple Western^TM. Simple Western lane view shows lysates of human small intestine tissue, loaded at 0.2 mg/mL. A specific band was detected for BTNL8 at approximately 60 kDa (as indicated) using 50 µg/mL of Rabbit Anti-Human BTNL8 Monoclonal Antibody (Catalog # MAB9359). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system. Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: BTNL8

Butyrophilin-like 8 (BTNL8) is a member of the BTN/MOG Ig-superfamily and functions as a negative regulator of immune cell activation (1). Human BTNL8 is a 500 amino acid (aa) type I transmembrane glycoprotein that contains a signal peptide followed by an extracellular domain (ECD), a transmembrane region and a short cytoplasmic domain (2). The ECD of human BTNL8 shares 88% sequence identity with the ECD of mouse BTNL8. BTNL8 has two alternatively spliced forms: B7-like and BTN-like. Both isoforms of BTNL8 are expressed in a range of human tissues (3). The complete immunological function of BTNL molecules is only beginning to emerge. BTNL8 has been shown to be important in initiation of primary immune responses, suggesting a role in priming of naïve T lymphocytes (3). Down-regulation of BNTL8 mRNA levels has been associated with ulcerative colitis and colon cancer (4). BTNL8 are expressed in colon, lung, testis and neutrophils, and its expression is significantly decreased in ulcerative colitis, colonic tumors as compared to unaffected tissue (4). Soluble BTNL8-Fc fusion protein binds to resting, but not activated T cells. In vitro, BTNL8 co-stimulates T cell proliferation and cytokine production. In vivo injections of BTNL8-Fc significantly increases production of
Ag-specific IgG during the primary but not the secondary immune response (3).

References