Human c-Abl Antibody Summary
Ala941-Val1140
Accession # NP_009297
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human c-Abl by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line, MCF-7 human breast cancer cell line, and MDA-MB-453 human breast cancer cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for c-Abl at approximately 135 - 145 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human c‑Abl by Simple WesternTM. Simple Western lane view shows lysates of Jurkat human acute T cell leukemia cell line, loaded at 0.2 mg/mL. A specific band was detected for c-Abl at approximately 149 kDa (as indicated) using 10 µg/mL of Goat Anti-Human c-Abl Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5414) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: c-Abl
c-Abl (Abelson murine leukemia viral homolog 1) is a cytosolic member of the ABL subfamily, protein tyrosine kinase family of enzymes. It is ubiquitously expressed, and participates in multiple processes such as cell migration, actin reorganization, DNA damage response and apoptosis. Human c-Abl (I-B) is 1149 amino acids (aa) in length. It is myristoylated on Gly2 and contains one SH3 domain (aa 80‑140), an SH2 domain (aa 146‑236), a protein kinase region (aa 261‑512), three NLS's (aa 620‑634; 726‑739; 778‑791), one DNA-binding region (aa 888‑988), an NES motif (aa 1109‑1119) and an actin F-binding domain (aa 1120‑1149). There is one alternate splice form (I-A) that contains a 26 aa substitution for the N-terminal 45 amino acids. In chronic myelogenous leukemia, c-Abl is fused to the Bcr gene product, resulting in the production of a constitutively active tyrosine kinase.
Product Datasheets
Citation for Human c-Abl Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
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Flow Cytometric Measurement of Blood Cells with BCR-ABL1 Fusion Protein in Chronic Myeloid Leukemia
Authors: L Löf, L Arngården, U Olsson-Str, B Siart, M Jansson, JS Dahlin, I Thörn, L Christians, M Hermansson, A Larsson, E Ahlstrand, G Wålinder, O Söderberg, R Rosenquist, U Landegren, M Kamali-Mog
Sci Rep, 2017-04-04;7(1):623.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
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Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma. There was no signal on the array.
