Human c-Myc PerCP-conjugated Antibody

Catalog #: IC3696C Datasheet / COA / SDS

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IC3696C

Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry.

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Human c-Myc PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects endogenous human c-Myc and c-Myc tagged proteins in Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 9E10

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

C-terminal region peptide of human c-Myc
Ala408-Ala439
Accession # P01106

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Intracellular Staining by Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry

Detection of c-Myc antibody in Jurkat Human Cell Line antibody by Flow Cytometry.

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Detection of c‑Myc in Jurkat Human Cell Line by Flow Cytometry. Jurkat human acute T cell leukemia cell line was stained with Mouse Anti-Human c-Myc PerCP-conjugated Monoclonal Antibody (Catalog # IC3696C, filled histogram) or isotype control antibody (Catalog # IC002C, open histogram). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (Catalog # FC004) and permeabilized with methanol. View our protocol for Staining Intracellular Molecules.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: c-Myc

Human c-Myc is a helix-loop-helix transcription factor which efficiently binds DNA after heterodimerization with the bHLH protein Max. It is often overexpressed and mutated in hematopoietic tumors. Mutations frequently result in truncation around amino acid (aa) 252, before the C-terminal DNA binding, HLH and leucine zipper domains. The 439 aa human c-Myc has one O-glycosylation site and has three Ser/Thr phosphorylation sites near the N-terminus. Human c-Myc shows 92% aa identity with mouse or rat c-Myc.

Long Name

v-Myc Avian Myelocytomatosis Viral Oncogene Homolog (Avian)

Entrez Gene IDs

4609 (Human); 17869 (Mouse); 24577 (Rat)

Alternate Names

avian myelocytomatosis viral oncogene homolog; BHLHE39; bHLHe39MRTL; Class E basic helix-loop-helix protein 39; cMyc; c-Myc; myc proto-oncogene protein; Myc; Myc2; MYCC; myc-related translation/localization regulatory factor; Niard; Nird; Proto-oncogene c-Myc; Transcription factor p64; v-myc avian myelocytomatosis viral oncogene homolog; v-myc myelocytomatosis viral oncogene homolog (avian)