Human C-Peptide Antibody

Catalog #: MAB80561
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB80561
MAB80561-SP

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Citations (1)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human C-Peptide Antibody Summary

Species Reactivity

Human

Specificity

Detects human C-Peptide in direct ELISAs.

Source

Monoclonal Mouse IgG₁ Clone # 915612

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human C-Peptide synthetic peptide
EAEDLQVGQVELGGGPGAGSLQPLALEGSLQ
Accession # P01308

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunohistochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry

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C-Peptide in Human Pancreas. C-Peptide was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human C-Peptide Monoclonal Antibody (Catalog # MAB80561) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to islets. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Peptide

Insulin is a peptide hormone that facilitates the cellular uptake of glucose by regulating the appearance of membrane glucose transporters. The single chain insulin propeptide consists of a 30 amino acid B chain (aa 25-54), a C-peptide (aa 55-89), and a 21 aa A chain (aa 90-110). Removal of the C-peptide by proteolysis enables the formation of mature Insulin, a disulfide-linked heterodimer of the A and B chains. Circulating C-peptide levels are elevated in hyperinsulinism, obesity, and type II diabetes. The human Insulin C-peptide shares 61% and 68% aa sequence identity with mouse and rat Insulin C-peptide, respectively.

Long Name

Proinsulin C-Peptide

Entrez Gene IDs

3630 (Human); 16333 (Mouse); 397415 (Porcine); 280829 (Bovine); 483665 (Canine)

Alternate Names

C-P; C-Peptide; IDDM2; ILPR; Insulin C-Peptide; insulin; IRDN; MODY10; Proinsulin C-Peptide; proinsulin

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human C-Peptide Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

A metabolic-dysfunction associated steatotic liver acinus biomimetic induces pancreatic islet dysfunction in a coupled microphysiology system
Authors: Aleman, J;Ravikumar, K;Wiegand, C;Schurdak, ME;Vernetti, L;Gavlock, D;Reese, C;DeBiasio, R;LaRocca, G;Angarita, YD;Gough, A;Soto-Gutierrez, A;Behari, J;Yechoor, V;Miedel, MT;Stern, AM;Banerjee, I;Taylor, DL;
bioRxiv : the preprint server for biology
Species: Human
Sample Types: Whole Tissue
Applications: Immunohistochemistry