Human C-Reactive Protein/CRP Antibody

Catalog #: MAB17073 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB17073-100
MAB17073-SP

Human C‑Reactive Protein/CRP ELISA Standard Curve.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human C-Reactive Protein/CRP Antibody Summary

Species Reactivity

Human

Specificity

Detects human C‑Reactive Protein/CRP in direct ELISAs.

Source

Monoclonal Mouse IgG_2B Clone # 987313

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human C‑Reactive Protein/CRP
Phe17-Pro224
Accession # P02741

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

ELISA

This antibody functions as an ELISA capture antibody when paired with Mouse Anti-Human C‑Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072).

This product is intended for assay development on various assay platforms requiring antibody pairs. We recommend the Human C-Reactive Protein/CRP DuoSet ELISA Kit (Catalog # DY1707) for convenient development of a sandwich ELISA or the Human C-Reactive Protein/CRP Quantikine ELISA Kit (Catalog # DCRP00) for a complete optimized ELISA.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

ELISA

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Human C‑Reactive Protein/CRP ELISA Standard Curve. Recombinant Human C-Reactive Protein/CRP protein was serially diluted 2-fold and captured by Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17073) coated on a Clear Polystyrene Microplate (Catalog # DY990). Mouse Anti-Human C-Reactive Protein/CRP Monoclonal Antibody (Catalog # MAB17072) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C-Reactive Protein/CRP

CRP is a secreted protein that belongs to the Pentraxin family. It circulates as a non-covalent homopentamer. The CRP concentration in human plasma is highly increased during acute phase response to tissue injury or infection.

Entrez Gene IDs

1401 (Human); 12944 (Mouse); 25419 (Rat)

Alternate Names

C-Reactive Protein; C-reactive protein, pentraxin-related; CRP; MGC88244; pentraxin 1; PTX1MGC149895