Human C1D Antibody

Catalog #: AF4816 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF4816
AF4816-SP

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Human C1D Antibody Summary

Species Reactivity

Human

Specificity

Detects human C1D in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human C1D
Ala2-Ser141
Accession # Q13901

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Immunogen affinity purified

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human C1D by Western Blot. Western blot shows lysates of JEG-3 human epithelial choriocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Goat Anti-Human C1D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4816) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for C1D at approximately 16 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: C1D

C1D (also SUN-CoR) is a 16 kDa member of the C1D family of DNA-binding proteins. It is ubiquitously expressed, activates DNA‑dependent protein kinase, and forms part of a large complex that processes rRNA. C1D exists as both a monomer and noncovalent homodimer, and is known to be phosphorylated. Human C1D is 141 amino acids (aa) in length. It contains two protein interaction sites at aa 50‑100 and 101‑141. There is one potential alternate site at Met54, and a number of single aa polymorphisms. Full-length C1D is 90% and 87% aa identical to mouse and canine C1D, respectively.

Long Name

C1D Nuclear DNA-binding Protein

Entrez Gene IDs

10438 (Human); 57316 (Mouse)

Alternate Names

C1D DNA-binding protein; C1D nuclear receptor corepressor; C1D nuclear receptor co-repressor; C1D; hC1D; MGC12261; MGC14659; nuclear DNA-binding protein; nuclear nucleic acid-binding protein C1D; small unique nuclear receptor corepressor; small unique nuclear receptor co-repressor; SUNCOR; SUN-CoR; SUNCORLRP1