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Human Cadherin-11 PE-conjugated Antibody

Catalog #: FAB1790P Datasheet / COA / SDS
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FAB1790P
Detection of Cadherin‑11 in NCI-H460 Human Cell Line by Flow Cytometry.
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Human Cadherin-11 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human Cadherin-11 in direct ELISAs and Western blots. In direct ELISAs, approximately 10% cross-reactivity with recombinant human (rh) Cadherin‑12, 5% cross-reactivity with rhK-Cadherin, and less than 2% cross-reactivity with rhCadherin-8, rhE‑Cadherin, rhN‑Cadherin, rhP‑Cadherin, rhVE‑Cadherin, rhCadherin-13, and rhCadherin‑17 is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
S. frugiperda insect ovarian cell line Sf 21-derived recombinant human Cadherin‑11
Phe23-Thr617
Accession # AAA35622
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry View Larger

Detection of Cadherin‑11 in NCI-H460 Human Cell Line by Flow Cytometry. NCI-H460 human large cell lung carcinoma cell line was stained with Goat Anti-Human Cadherin-11 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1790P, filled histogram) or isotype control antibody (IC108P, open histogram). Staining was performed using our protocol for Staining Membrane-associated Proteins protocol.

Flow Cytometry Detection of Cadherin-11 antibody in PC-3 Human Cell Line antibody by Flow Cytometry. View Larger

Detection of Cadherin‑11 in PC‑3 Human Cell Line by Flow Cytometry. PC-3 human prostate cancer cell line was stained with Goat Anti-Human Cadherin-11 PE-conjugated Antigen Affinity-purified Polyclonal Antibody (Catalog # FAB1790P, filled histogram) or isotype control antibody (Catalog # IC108P, open histogram). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: Cadherin-11

Cadherin-11, also known as OB-Cadherin, is a 120 kDa member of the classical Cadherin family of calcium-dependent homophilic adhesion proteins. Cadherins are involved in multiple processes including embryonic development, cell migration, and maintenance of epithelial integrity (1). Cadherin-11 is expressed in embryonic mesodermal tissues and contributes to the morphogenesis of the nervous and skeletal systems (2-4). It is expressed on osteoblasts in the adult where it promotes the differentiation of both osteoblasts and chondrocytes (5). Cadherin-11 is up‑regulated on breast cancer and prostate cancer cells which preferentially metastasize to bone (6, 7). It facilitates this metastasis via homophilic adhesion to bone marrow stroma and osteoblast-expressed Cadherin-11 (6‑8). In the synovium, Cadherin‑11 supports adhesion between synoviocytes but promotes cell invasion in synovitis and rheumatoid arthritis (9, 10). Its up‑regulation in the vasculature following injury contributes to intimal hyperplasia by inducing smooth muscle cell migration and proliferation (11). In the nervous system, Cadherin-11 interacts with FGF R1 to promote neurite extension from spinal cord explants (12). Mature human Cadherin‑11 consists of a 564 amino acid (aa) extracellular domain (ECD) with five tandem Cadherin repeats, a 23 aa transmembrane segment, and a 156 aa cytoplasmic domain (13, 14). Within the ECD, human Cadherin-11 shares 97% and 98% aa sequence identity with mouse and rat Cadherin‑11, respectively. An 80 kDa portion of the Cadherin‑11 ECD can be shed by proteolytic cleavage, and this fragment competes with the full length molecule for cell adhesion (3, 15). Alternate splicing of human Cadherin-11 generates an 85 kDa isoform with substituted transmembrane and cytoplasmic regions (14, 15).

References
  1. Pokutta, S. and W.I. Weis (2007) Annu. Rev. Cell Dev. Biol. 23:237.
  2. Kimura, Y. et al. (1995) Dev. Biol. 169:347.
  3. McCusker, C. et al. (2009) Mol. Biol. Cell 20:78.
  4. Clendenon, S.G. et al. (2009) Dev. Dyn. 238:1909.
  5. Kii, I. et al. (2004) J. Bone Mineral Res. 19:1840.
  6. Tamura, D. et al. (2008) Int. J. Oncol. 33:17.
  7. Chu, K. et al. (2008) Mol. Cancer Res. 6:1259.
  8. Huang, C.-F. et al. (2010) Cancer Res. 70:4580.
  9. Valencia, X. et al. (2004) J. Exp. Med. 200:1673.
  10. Kiener, H.P. et al. (2009) Arthritis Rheum. 60:1305.
  11. Monahan, T.S. et al. (2007) J. Vasc. Surg. 45:581.
  12. Boscher, C. and R.-M. Mege (2008) Cell. Signal. 20:1061.
  13. Tanihara, H. et al. (1994) Cell Adhes. Commun. 2:15.
  14. Okazaki, M. et al. (1994) J. Biol. Chem. 269:12092.
  15. Kawaguchi, J. et al. (1999) J. Bone Mineral Res. 14:764.
Entrez Gene IDs
1009 (Human); 12552 (Mouse)
Alternate Names
CAD11OSF-4; cadherin 11, type 2, OB-cadherin (osteoblast); Cadherin11; Cadherin-11; CDH11; CDHOB; OB; OB-Cadherin; Osteoblast cadherin

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