Human Calbindin D Antibody Summary
Ala2-Asn261
Accession # P05937
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human Calbindin D by Western Blot. Western blot shows human urine and lysates of human kidney tissue. PVDF membrane was probed with 5 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Calbindin D at approximately 25 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human Calbindin D by Simple WesternTM. Simple Western lane view shows lysates of human brain (cerebellum) tissue, loaded at 0.2 mg/mL. A specific band was detected for Calbindin D at approximately 32 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
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Calbindin D in Human Brain (Hippocampus). Calbindin D was detected in immersion fixed paraffin-embedded sections of human brain (hippocampus) using Goat Anti-Human Calbindin D Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3320) at 3 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Goat IgG VisUCyte™ HRP Polymer Antibody (VC004). Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (CTS013). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to neuronal cell bodies and synaptic vesicles. Staining was performed using our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Calbindin D
Calbindin D, also known as Calbindin 1, is a Vitamin D-dependent cytoplasmic protein in the EF-hand calcium binding protein family that includes calmodulin, parvalbumin, troponin C, and S100. Calbindin D is widely expressed and regulates calcium homeostasis. Calbindin D protects neurons from excitotoxic and apoptotic cell death by buffering excess calcium. Human, mouse, and rat Calbindin D share 98% amino acid sequence identity.
Product Datasheets
Citations for Human Calbindin D Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 6
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Mechanically Activated Piezo Channels Mediate Touch and Suppress Acute Mechanical Pain Response in Mice
Authors: M Zhang, Y Wang, J Geng, S Zhou, B Xiao
Cell Rep, 2019-02-05;26(6):1419-1431.e4.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Cerebrospinal fluid calbindin D concentration as a biomarker of cerebellar disease progression in Niemann-Pick type C1 disease
Authors: Allison M Bradbury
J Pharmacol Exp Ther, 2016-06-15;0(0):.
Species: Human
Sample Types: CSF
Applications: ELISA Development (Detection) -
Rgs6 is required for adult maintenance of dopaminergic neurons in the ventral substantia nigra.
Authors: Bifsha P, Yang J, Fisher R, Drouin J
PLoS Genet, 2014-12-11;10(12):e1004863.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P -
Unbiased classification of sensory neuron types by large-scale single-cell RNA sequencing.
Authors: Usoskin D, Furlan A, Islam S, Abdo H, Lonnerberg P, Lou D, Hjerling-Leffler J, Haeggstrom J, Kharchenko O, Kharchenko P, Linnarsson S, Ernfors P
Nat Neurosci, 2014-11-24;18(1):145-53.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Otx2 cell-autonomously determines dorsal mesencephalon versus cerebellum fate independently of isthmic organizing activity.
Authors: Di Giovannantonio, Luca G, Di Salvio, Michela, Omodei, Daniela, Prakash, Nilima, Wurst, Wolfgang, Pierani, Alessand, Acampora, Dario, Simeone, Antonio
Development, 2013-12-11;141(2):377-88.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC -
Otx2 controls neuron subtype identity in ventral tegmental area and antagonizes vulnerability to MPTP.
Authors: Di Salvio M, Di Giovannantonio LG, Acampora D
Nat. Neurosci., 2010-11-07;13(12):1481-8.
Species: Mouse
Sample Types: Whole Tissue
Applications: IHC-P
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