Human Caldesmon/CALD1 Antibody

Catalog #: MAB7569
1 Citation Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB7569
MAB7569-SP

Detection of Human Caldesmon/CALD1 by Western Blot.

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Human Caldesmon/CALD1 Antibody Summary

Species Reactivity

Human

Specificity

Detects human Caldesmon/CALD1 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG_2B Clone # 767001

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli-derived recombinant human Caldesmon/CALD1
Lys696-Val793
Accession # Q05682

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Immunohistochemistry

3-25 µg/mL

Immersion fixedparaffin-embedded sections of endometrial carcinoma and melanoma

Immunocytochemistry

3-25 µg/mL

Immersion fixed WI‑38 human lung fibroblast cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Caldesmon/CALD1 by Western Blot. Western blot shows lysates of WI-38 human lung fibroblast cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF018). A specific band was detected for Caldesmon/CALD1 at approximately 80 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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Caldesmon/CALD1 in WI‑38 Human Cell Line. Caldesmon/CALD1 was detected in immersion fixed WI-38 human lung fibroblast cell line using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counter-stained with DAPI (blue). Specific staining was localized to cytoskeleton. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Immunohistochemistry

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Detection of Caldesmon/CALD1 in Endometrial Carcinoma. Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of endometrial carcinoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunocytochemistry

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Detection of Caldesmon/CALD1 in Endometrial Carcinoma. Caldesmon/CALD1 was detected in immersion fixed paraffin-embedded sections of melanoma using Mouse Anti-Human Caldesmon/CALD1 Monoclonal Antibody (Catalog # MAB7569) at 1 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the Anti-Mouse HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS002) and counterstained with hematoxylin (blue). Specific staining was localized to smooth muscle. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caldesmon/CALD1

Caldesmon, also known as CaD or h-CaD, is a 120-150 kDa cytosolic protein that is important in actin cytoskeleton dynamics. Smooth muscle Caldesmon regulates calcium-dependent smooth muscle contraction by stabilizing actin filaments, inhibiting the actomyosin ATPase, and interacting with F-actin, myosin, tropomyosin, and calmodulin. It is also a key molecule in other related actin-related processes including cytokinesis, cell migration, wound healing, and exocytosis. Caldesmon activity is regulated by serine and tyrosine phosphorylation by multiple kinases. Alternate splicing generates 70-80 kDa isoforms of human Caldesmon, known as I-CaD, that lack the central repeating region of the protein (aa 208-436 or aa 208-462). These isoforms are widely expressed and are comparable in size to full length mouse and rat Caldesmon. Within aa 696-793, human Caldesmon shares 94% and 95% aa sequence identity with mouse and rat Caldesmon, respectively.

Entrez Gene IDs

800 (Human)

Alternate Names

CAD; CALD1; caldesmon 1; Caldesmon; CDM; CDMH-CAD; HCAD; LCAD; L-CAD; MGC21352; NAG22

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Citation for Human Caldesmon/CALD1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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