Human/Canine Integrin beta 1/CD29 Antibody

Catalog #: AF1778
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF1778
AF1778-SP

Detection of Human Integrin beta 1/CD29 by Western Blot.

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human/Canine Integrin beta 1/CD29 Antibody Summary

Species Reactivity

Human, Canine

Specificity

Detects human Integrin beta 1/CD29 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Integrin beta 1 isoform 1A
Gln21-Asp728
Accession # P05556

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

Please refer to the PDF datasheet for specifications.

Simple Western

10 µg/mL

See below

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Immunocytochemistry

5-15 µg/mL

See below

Knockout Validated

Integrin beta 1/CD29 is specifically detected in A549 human lung carcinoma parental cell line but is not detectable in Integrin beta 1/CD29 knockout A549 cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human Integrin beta 1/CD29 by Western Blot. Western blot shows lysates of MG-63 human osteosarcoma cell line, HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 130-140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Flow Cytometry

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Detection of Integrin beta 1/CD29 in Canine PBMCs by Flow Cytometry. Canine peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Flow Cytometry

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Detection of Integrin beta 1/CD29 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Flow Cytometry

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Detection of Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells by Flow Cytometry. Canine mesenchymal stem cells were stained with Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778, filled histogram) or isotype control antibody (Catalog # AB-108-C, open histogram), followed by Phycoerythrin-conjugated Anti-Goat IgG Secondary Antibody (Catalog # F0107).

Immunocytochemistry

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Integrin beta 1/CD29 in Canine Mesenchymal Stem Cells. Integrin beta 1/CD29 was detected in immersion fixed canine mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry

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Integrin beta 1/CD29 in Human Mesenchymal Stem Cells. Integrin beta 1/CD29 was detected in immersion fixed human mesenchymal stem cells using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.

Immunocytochemistry

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Integrin beta 1/CD29 in A549 Human Cell Line. Integrin beta 1/CD29 was detected in immersion fixed A549 human lung carcinoma cell line using Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) at 5 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Simple Western

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Detection of Human Integrin beta 1/CD29 by Simple Western^TM. Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line, A431 human epithelial carcinoma cell line, Jurkat human acute T cell leukemia cell line, and U-87 MG human glioblastoma/astrocytoma cell line, loaded at 0.2 mg/mL. Specific bands were detected for Integrin beta 1/CD29 at approximately 157-176 kDa (as indicated) using 10 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Knockout Validated

Western Blot Shows Human Integrin beta 1/CD29 Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Integrin beta 1/CD29 Specificity by Using Knockout Cell Line. Western blot shows lysates of A549 human lung carcinoma parental cell line and Integrin beta 1/CD29 knockout A549 cell line (KO). PVDF membrane was probed with 1 µg/mL of Goat Anti-Human/Canine Integrin beta 1/CD29 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1778) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). Specific bands were detected for Integrin beta 1/CD29 at approximately 105 kDa and 120 kDa (as indicated) in the parental A549 cell line, but is not detectable in knockout A549 cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Integrin beta 1/CD29

Integrin beta 1, also called CD29 and VLA-beta chain, associates with at least ten different integrin alpha subunits to form various VLA complexes. The beta 1 subunit has a broad tissue distribution except erythrocytes. Over aa 21-720, human Integrin beta 1 shares 95% aa identity with canine Integrin beta 1.

Entrez Gene IDs

3688 (Human); 16412 (Mouse); 102144610 (Cynomolgus Monkey)

Alternate Names

CD29 antigen; CD29; Fibronectin receptor subunit beta; FNRBVLAB; GPIIA; Integrin beta 1; integrin beta-1; integrin VLA-4 beta subunit; integrin, beta 1 (fibronectin receptor, beta polypeptide, antigen CD29 includesMDF2, MSK12); ITGB1; MDF2; MSK12; very late activation protein, beta polypeptide; VLA-4 subunit beta; VLA-BETA

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human/Canine Integrin beta 1/CD29 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Reparative effect of mesenchymal stromal cells on endothelial cells after hypoxic and inflammatory injury
Authors: JM Sierra-Par, A Merino, M Eijken, H Leuvenink, R Ploeg, BK Møller, B Jespersen, CC Baan, MJ Hoogduijn
Stem Cell Res Ther, 2020-08-12;11(1):352.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Bystander cells enhance NK cytotoxic efficiency by reducing search time
Authors: X Zhou, R Zhao, K Schwarz, M Mangeat, EC Schwarz, M Hamed, I Bogeski, V Helms, H Rieger, B Qu
Sci Rep, 2017-03-13;7(0):44357.
Species: Human
Sample Types: Whole Cells
Applications: Migration Assay
In vivo biomarker expression patterns are preserved in 3D cultures of Prostate Cancer.
Authors: Windus L, Kiss D, Glover T, Avery V
Exp Cell Res, 2012-07-27;318(19):2507-19.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot