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Human Caspase-1 Antibody

Catalog #: MAB6215
16 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB6215
MAB6215-SP
Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot.
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Product Details
Citations (16)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Caspase-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Caspase-1 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG2A Clone # 661228
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E.coli-derived recombinant human Caspase-1
Asn120-Asp297
Accession # P29466
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
See below
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human, Mouse, and Rat Caspase-1 antibody by Western Blot. View Larger

Detection of Human, Mouse, and Rat Caspase‑1 by Western Blot. Western blot shows lysates of A431 human epithelial carcinoma cell line, NIH-3T3 mouse embryonic fibroblast cell line, and Rat-2 rat embryonic fibroblast cell line. PVDF membrane was probed with 0.1 µg/mL of Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Caspase-1 at approximately 45 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 2.

Immunocytochemistry Caspase-1 antibody in THP-1 Human Cell Line by Immunocytochemistry (ICC). View Larger

Caspase‑1 in THP‑1 Human Cell Line. Caspase-1 was detected in immersion fixed THP-1 human acute monocytic leukemia cell line using Mouse Anti-Human Caspase-1 Monoclonal Antibody (Catalog # MAB6215) at 15 µg/mL for 3 hours at room temperature. Cells were stained using the Northern-Lights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (yellow; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Caspase-1

Caspase-1, also known as IL-1 beta -converting enzyme (ICE), is an aspartic protease that plays a key role in the inflammatory response and apoptosis. Caspase-1 precursor (about 50kDa) can be cleaved and the active enzyme consists of a complex of two 20 kDa (aa 120-297) and two 10 kDa (aa 317-404) subunits which associate following cleavage of inactive precursors. Caspase-1 is required for proteolytic cleavage of the IL-1 beta precursor to form the active proinflammatory cytokine. Alternate splicing generates several additional Caspase-1 isoforms with deletions in the propeptide regions or also in the mature subunits. Within the large subunit, human Caspase 1 shares 61% aa sequence identity with mouse and rat Caspase-1.

Entrez Gene IDs
834 (Human)
Alternate Names
CASP1 nirs variant 1; CASP1; CASP-1; caspase 1, apoptosis-related cysteine peptidase; Caspase-1 subunit p10; Caspase-1 subunit p20; Caspase1; Caspase-1; EC 3.4.22.36; EC:3.4.22.36; ICE; IL-1 beta-converting enzyme; IL1BC; IL-1BC; IL1BCE; IL1B-convertase; interleukin 1, beta, convertase; interleukin 1-B converting enzyme; Interleukin-1 beta convertase; Interleukin-1 beta-converting enzyme; p45

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Citations for Human Caspase-1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

16 Citations: Showing 1 - 10
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  1. Herpes Simplex Virus ICP27 Protein Inhibits AIM 2-Dependent Inflammasome Influencing Pro-Inflammatory Cytokines Release in Human Pigment Epithelial Cells (hTert-RPE 1)
    Authors: Caproni, A;Nordi, C;Fontana, R;Facchini, M;Melija, S;Pappadà, M;Buratto, M;Marconi, P;
    International journal of molecular sciences
    Species: Primate - Cercopithecus aethiops (African Green Monkey)
    Sample Types: Cell Lysates
    Applications: Western Blot
  2. Anti-Inflammatory Neutrophils Reprogram Macrophages toward a Pro-Healing Phenotype with Increased Efferocytosis Capacity
    Authors: Mihaila, AC;Ciortan, L;Tucureanu, MM;Simionescu, M;Butoi, E;
    Cells
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  3. The FXR mediated anti-depression effect of CDCA underpinned its therapeutic potentiation for MDD
    Authors: H Li, X Zhu, J Xu, L Li, W Kan, H Bao, J Xu, W Wang, Y Yang, P Chen, Y Zou, Y Feng, J Yang, J Du, G Wang
    International immunopharmacology, 2022-12-28;115(0):109626.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  4. Acyl-Coenzyme A Synthetase Long-Chain Family Member 4 Is Involved in Viral Replication Organelle Formation and Facilitates Virus Replication via Ferroptosis
    Authors: YA Kung, HJ Chiang, ML Li, YN Gong, HP Chiu, CT Hung, PN Huang, SY Huang, PY Wang, TA Hsu, G Brewer, SR Shih
    MBio, 2022-01-18;0(0):e0271721.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  5. AIM2 forms a complex with pyrin and ZBP1 to drive PANoptosis and host defence
    Authors: S Lee, R Karki, Y Wang, LN Nguyen, RC Kalathur, TD Kanneganti
    Nature, 2021-09-01;0(0):.
    Species: Human, Mouse, Transgenic Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  6. Arf6 exacerbates allergic asthma through cell-to-cell transmission of ASC inflammasomes
    Authors: S Lee, A Ishitsuka, T Kuroki, YH Lin, A Shibuya, T Hongu, Y Funakoshi, Y Kanaho, K Nagata, A Kawaguchi
    JCI Insight, 2021-08-23;6(16):.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  7. Autophagy-based unconventional secretion of HMGB1 by keratinocytes plays a pivotal role in psoriatic skin in?ammation
    Authors: Z Wang, H Zhou, H Zheng, X Zhou, G Shen, X Teng, X Liu, J Zhang, X Wei, Z Hu, F Zeng, Y Hu, J Hu, X Wang, S Chen, J Cheng, C Zhang, Y Gui, S Zou, Y Hao, Q Zhao, W Wu, Y Zhou, K Cui, N Huang, Y Wei, W Li, J Li
    Autophagy, 2020-02-16;0(0):1-24.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  8. Hemoglobin oxidation generates globin-derived peptides in atherosclerotic lesions and intraventricular hemorrhage of the brain, provoking endothelial dysfunction
    Authors: N Posta, É Cs?sz, M Oros, D Peth?, L Potor, G Kalló, Z Hendrik, KÉ Sikura, G Méhes, C Tóth, J Posta, G Balla, J Balla
    Lab. Invest., 2020-02-13;0(0):.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  9. IL-1? activation in response to Staphylococcus aureus lung infection requires inflammasome-dependent and independent mechanisms
    Authors: S Pires, D Parker
    Eur. J. Immunol., 2018-08-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  10. The Vasoreparative Function of Myeloid Angiogenic Cells Is Impaired in Diabetes Through the Induction of IL1?
    Authors: SEJ Chambers, CL O'Neill, J Guduric-Fu, KJ McLoughlin, A Liew, AM Egan, T O'Brien, AW Stitt, RJ Medina
    Stem Cells, 2018-03-09;36(6):834-843.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  11. Targeting the deubiquitinase STAMBP inhibits NALP7 inflammasome activity
    Authors: JS Bednash, N Weathingto, J Londino, M Rojas, DL Gulick, R Fort, S Han, AC McKelvey, BB Chen, RK Mallampall
    Nat Commun, 2017-05-11;8(0):15203.
    Species: Human
    Sample Types: Cell Lysates
    Applications: Western Blot
  12. AIM2 Inflammasome Is Critical for Influenza-Induced Lung Injury and Mortality
    Authors: H Zhang, J Luo, JF Alcorn, K Chen, S Fan, J Pilewski, A Liu, W Chen, JK Kolls, J Wang
    J. Immunol., 2017-04-19;0(0):.
    Species: Mouse
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  13. HMGB1 Induces an Inflammatory Response in the Chorioamniotic Membranes That Is Partially Mediated by the Inflammasome
    Authors: Nardhy Gomez-Lope
    Biol. Reprod., 2016-11-02;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  14. Monocyte Caspase-1 Is Released in a Stable, Active High Molecular Weight Complex Distinct from the Unstable Cell Lysate-Activated Caspase-1.
    Authors: Shamaa O, Mitra S, Gavrilin M, Wewers M
    PLoS ONE, 2015-11-24;10(11):e0142203.
    Species: Human
    Sample Types: Cell Culture Supernates
    Applications: Immunoprecipitation
  15. Age-related increases in amyloid beta and membrane attack complex: evidence of inflammasome activation in the rodent eye.
    Authors: Zhao T, Gao J, Van J, To E, Wang A, Cao S, Cui J, Guo J, Lee M, McGeer P, Matsubara J
    J Neuroinflammation, 2015-06-24;12(0):121.
    Species: Rat
    Sample Types: Tissue Homogenates
    Applications: Western Blot
  16. Propionibacterium acnes Induces IL-1beta secretion via the NLRP3 inflammasome in human monocytes.
    Authors: Qin M, Pirouz A, Kim M, Krutzik S, Garban H, Kim J
    J Invest Dermatol, 2013-07-24;134(2):381-8.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P

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Human Caspase-1 Antibody
By Anonymous on 10/06/2021
Application: ICC/IF Sample Tested: Spinal motor neurons Species: Human
Immunocytochemistry/Immunofluorescence Human Caspase-1 Antibody MAB6215