Human Cathepsin S Antibody

Name: Human Cathepsin S Antibody
Brand: R&D Systems
SKU: MAB1183
Rating: 3.7 (3 reviews)

Catalog #: MAB1183
1 Citation 3 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1183
MAB1183-SP

All Cathepsin S Antibodies

Product Details

Citations (1)

FAQs

Supplemental Products

Reviews (3)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Cathepsin S Antibody Summary

Species Reactivity

Human

Specificity

Detects pro and mature forms of human Cathepsin S in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human Cathepsin B, C, D, L, L2/V, X/Z/P, recombinant mouse Cathepsin F, or H is observed.

Source

Monoclonal Rat IgG_2B Clone # 248718

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin S
Gln17-Ile331 (predicted)
Accession # P25774

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human Cathepsin S (Catalog # 1183-CY)

Immunoprecipitation

25 µg/mL

Conditioned cell culture medium spiked with Recombinant Human Cathepsin S (Catalog # 1183-CY), see our available Western blot detection antibodies

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Cathepsin S

Cathepsin S is a lysosomal cysteine protease of the papain family (1). It plays a major role in the processing of the MHC class II-associated invariant chain (2). It has been implicated in the pathogenesis of several diseases such as Alzheimer’s disease and degenerative disorders associated with the cells of the mononuclear phagocytic system (1). Human Cathepsin S is synthesized as a preproenzyme of 331 amino acid residues consisting a signal peptide (residues 1‑16), a pro region (residues 17‑114), and the mature enzyme (residues 115‑331) (3‑5). Cathepsin S is less abundant in tissues than Cathepsins B, L and H. The highest levels have been found in lymph nodes, spleen, macrophages and other phagocytic cells.

References

Kirschke, H. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, A.J. et al.) pp. 1104, Academic Press, San Diego.
Turk, V. et al. (2001) EMBO J. 20:4629.
Shi, G.P. et al. (1992) J. Biol. Chem. 267:7258.
Shi, G.P. et al. (1994) J. Biol. Chem. 269:11530.
Wiederanders, B. et al. (1992) J. Biol. Chem. 267:13708.

Entrez Gene IDs

1520 (Human)

Alternate Names

Cathepsin S; CTSS; EC 3.4.22; EC 3.4.22.27; FLJ50259; MGC3886

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citation for Human Cathepsin S Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1