Human CCL14a/HCC-1 Antibody

Catalog #: AF-324-PB Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF-324-PB
AF-324-SP
Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14a/HCC‑1 Antibody.
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Human CCL14a/HCC-1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL14a/HCC‑1 in direct ELISAs and Western blots. In direct ELISAs and Western blots (non-reducing conditions), less than 5% cross-reactivity with recombinant human (rh) MCP-2, rhMCP-3, recombinant mouse (rm) C10, and rmMIP-1 beta is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL14a/HCC‑1
Thr20-Asn93
Accession # Q16627
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL14a/HCC‑1 aa 20-93 (Catalog # 324-HC)
Neutralization
Measured by its ability to neutralize CCL14a/HCC‑1-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 0.15-0.75 µg/mL in the presence of 10 ng/mL Recombinant Human CCL14a/HCC‑1 aa 28‑93.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14a/HCC‑1 Antibody. View Larger

Chemotaxis Induced by CCL14a/HCC‑1 and Neutral-ization by Human CCL14a/HCC‑1 Antibody. Recombinant Human CCL14a/HCC-1 (Catalog # 1578-HC) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL14a/HCC-1 (10 ng/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL14a/ HCC-1 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF-324-PB). The ND50 is typically 0.15-0.75 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL14a/HCC-1

HCC-1 (Hemofiltrate CC Chemokine-1) was originally isolated from the hemofiltrate of human patients with chronic renal failure (1). It belongs to the CC chemokine superfamily and has been designated CCL14a. HCC-1/CCL14a cDNA encodes a 93 amino acid (aa) residue  precursor with a 19 aa signal peptide that is cleaved to form the 74 aa secreted protein (aa 20‑93). By alternative splicing, a second longer isoform named HCC-3/CCL14b, which includes sequences from exon 3, also exists (2). HCC-1/CCL14a is expressed constitutively in various normal tissues including spleen, liver, muscle, gut and bone marrow. It circulates at nanomolar concentrations in human plasma. Different post-translationally modified HCC-1/CCL14a, including O-glycosylated and N-terminally truncated variants of HCC‑1/CCL14a, have been identified (3, 4). Whereas the 74 aa peptide is a weak CCR1 agonist, the proteolytically processed, truncated HCC‑1/CCL14a (aa 28‑93) is a highly potent agonist of CCR1, CCR5 and to a lesser extent, CCR3. HCC-1/CCL14a (aa 28‑93) promotes chemotaxis of T lymphocytes, monocytes and eosinophils, and inhibits infection of M-tropic human immunodeficiency virus type 1. Activation of the HCC-1/CCL14a precursor to active peptide is mediated by the urokinase type plasminogen activator or plasmin (5).

References
  1. Schulz-Knappe, P. et al. (1996) J. Exp. Med. 183:295.
  2. Forssmanns, U. et al. (2001) J. Leukocyte Biology 70:357.
  3. Richter, R. et al. (2000) Biochemistry 39:10799.
  4. Munch, J. et al. (2002) Antimicrob. Agents Chemother. 46:982.
  5. Vakili, J. et al. (2001) J. Immunol. 167:3406.
Entrez Gene IDs
6358 (Human)
Alternate Names
C-C motif chemokine 14;CC-1;CC-3;CKB1;HCC-1;HCC-1(1-74);HCC-1/HCC-3;HCC-3;MCIF;NCC2;NCC-2;SCYA14;SCYL2;SY14; CCL14; CCL14a; HCC1; HCC-1

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