Home / CCL18/PARC / Human CCL18/PARC Biotinylated Antibody

Human CCL18/PARC Biotinylated Antibody

Catalog #: BAF394
4 Citations Datasheet / COA / SDS
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BAF394
Detection of CCL18/PARC in Human Macrophages by Flow Cytometry.
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Citations (4)
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Human CCL18/PARC Biotinylated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL18/PARC in ELISAs and Western blots. In sandwich ELISAs, less than 0.1% cross-reactivity with recombinant human (rh) HCC‑1, rhMPIF‑1, rhMIP‑1 alpha, recombinant mouse (rm) MIP‑1 alpha, rhMIP‑1 beta, rmMIP‑1 beta, recombinant viral (rv) MIP‑I, rvMIP‑II, and rhMIP‑1δ is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL18/PARC
Ala21-Ala89
Accession # P55774
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
Label
Biotin

Applications

Recommended Concentration
Sample
Western Blot
0.1 µg/mL
Recombinant Human CCL18/PARC (Catalog # 394-PA)
Intracellular Staining by Flow Cytometry
0.25 µg/106 cells
Human macrophages generated from positively selected (Catalog # MAGH105) CD14+ PBMC cultured with Recombinant Human M-CSF (Catalog # 216-MC), Recombinant Human IL-10 (Catalog # 217-IL) and  Recombinant Human IL‑4 (Catalog # 204-IL), fixed with paraformaldehyde and permeabilized with saponin

Human CCL18/PARC Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Detection (Matched Antibody Pair)
0.1-0.4 µg/mL 

Use in combination with:

Capture Reagent: Human CCL18/PARC Antibody (Catalog # MAB394)

Standard: Recombinant Human CCL18/PARC Protein (Catalog # 394-PA)

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Intracellular Staining by Flow Cytometry Detection of CCL18/PARC antibody in Human Macrophages antibody by Flow Cytometry. View Larger

Detection of CCL18/PARC in Human Macrophages by Flow Cytometry. Macrophages were generated from positively selected (MAGH105) CD14+ human PBMC, cultured with 50 ng/ml rhM-CSF (216-MC), 10 ng/ml rhIL-10 (217-IL) and 10 ng/ml rhIL-4 (204-IL) for 6 days, then treated with 3nM Monensin overnight and stained with (A) Goat Anti-Human CCL18/PARC Biotinylated Polyclonal Antibody (Catalog # BAF394) or (B) control antibody (BAF108), followed by Allophycocyanin-conjugated Streptavidin (F0050) and Mouse anti-Human CD163 Alexa Fluor® 488-conjugated Monoclonal Antibody (FAB1607G). To facilitate intracellular staining, cells were fixed with Flow Cytometry Fixation Buffer (FC004) and permeabilized with Flow Cytometry Permeabilization/Wash Buffer I (FC005). Staining was performed using our Staining Intracellular Molecules protocol.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL18/PARC

CCL18, also known as alternative macrophage activation-associated CC chemokine (AMAC)-1, macrophage inflammatory protein (MIP)-4, and dendritic cell chemokine (DC-CK1), is a novel CC chemokine that is highly homologous to MIP-1 alpha (61% amino acid (aa) sequence identity). CCL18 cDNA encodes an 89 aa residue precursor protein with a 20 aa putative signal peptide that is cleaved to generate a 69 aa residue mature protein which lacks potential glycosylation sites. In vitro, CCL18 mRNA expression is induced in alternatively activated macrophages by Th2 cytokines such as IL-4, IL-10, and IL-13, and inhibited by IFN‑ gamma. CCL18 mRNA is also expressed by GM-CSF/IL-4-induced monocyte-derived dendritic cells. In vivo, CCL18 is highly expressed in lung and placenta but is not expressed in epidermal Langerhans cells. Recombinant CCL18 has been shown to chemoattract naive T cells but not monocytes or neutrophils.

References
  1. Adema, G. et al. (1997) Nature 387:713.
  2. Kodelja, V. et al. (1998) J. Immunol. 160:141.
Entrez Gene IDs
6362 (Human)
Alternate Names
Alternative macrophage activation-associated CC chemokine 1; AMAC-1; AMAC1CC chemokine ligand 18; AMAC-1Small-inducible cytokine A18; CCL18; chemokine (C-C motif) ligand 18 (pulmonary and activation-regulated); CKb7; DC-CK1; DC-CK1CC chemokine PARC; DCCK1Macrophage inflammatory protein 4; Dendritic cell chemokine 1; MIP4; MIP-4; MIP-4C-C motif chemokine 18; PARC; PARCPulmonary and activation-regulated chemokine; SCYA18chemokine (C-C), dendritic; small inducible cytokine A18; small inducible cytokine subfamily A (Cys-Cys), member 18, pulmonary andactivation-regulated

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Citations for Human CCL18/PARC Biotinylated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Prominent Levels of the Profibrotic Chemokine CCL18 during Peritonitis: In Vitro Downregulation by Vitamin D Receptor Agonists
    Authors: M Ossorio, V Martínez, MA Bajo, G Del Peso, MJ Castro, S Romero, R Selgas, T Bellón
    Biomed Res Int, 2018-04-04;2018(0):6415892.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Multiplex serum protein analysis reveals potential mechanisms and markers of response to hyperimmune caprine serum in systemic sclerosis
    Authors: N Quillinan, KE Clark, B Youl, J Vernes, D McIntosh, S Haq, CP Denton
    Arthritis Res. Ther, 2017-03-07;19(1):45.
    Species: Human
    Sample Types: Serum
    Applications: ELISA Development (Detection)
  3. Osseous manifestations of adult Gaucher disease in the era of enzyme replacement therapy.
    Authors: Deegan PB, Pavlova E, Tindall J
    Medicine (Baltimore), 2011-01-01;90(1):52-60.
    Species: Human
    Sample Types: Plasma
    Applications: Complex Application
  4. Potential biomarkers of osteonecrosis in Gaucher disease.
    Authors: Pavlova EV, Deegan PB, Tindall J, McFarlane I, Mehta A, Hughes D, Wraith JE, Cox TM
    Blood Cells Mol. Dis., 2010-11-13;46(1):27-33.
    Species: Human
    Sample Types: Serum
    Applications: Luminex Development

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