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Human CCL19/MIP-3 beta Antibody

Catalog #: AF361
9 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
AF361
AF361-SP
Detection of Human CCL19/MIP‑3 beta by Western Blot.
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Citations (9)
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Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL19/MIP-3 beta Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCL19/MIP-3 beta in ELISAs and Western blots. In sandwich immunoassays, less than 0.05% cross-reactivity with recombinant mouse (rm) MIP‑3 beta, recombinant human (rh) GRO alpha, rhHCC-4, rhEotaxin, rmMIP-3 alpha, recombinant rat MIP-3 alpha, and rhMIP-3 alpha is observed.
Source
Polyclonal Goat IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human CCL19/MIP-3 beta (R&D Systems, Catalog # 361-MI)
Gly22-Ser98
Accession # Q99731
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
See below
Simple Western
50 µg/mL
See below

Human CCL19/MIP-3 beta Sandwich Immunoassay

Recommended Concentration
Reagent
ELISA Capture (Matched Antibody Pair)
0.2-0.8 µg/mL 

Use in combination with:

Detection Reagent: Human CCL19/MIP‑3 beta Biotinylated Antibody (Catalog # BAF361)

Standard: Recombinant Human CCL19/MIP-3 beta Protein (Catalog # 361-MI)

Neutralization
Measured by its ability to neutralize CCL19/MIP‑3 beta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR7. The Neutralization Dose (ND50) is typically 2-10 µg/mL in the presence of 50 ng/mL Recombinant Human CCL19/MIP‑3 beta.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Human CCL19/MIP-3 beta antibody by Western Blot. View Larger

Detection of Human CCL19/MIP‑3 beta by Western Blot. Western blot shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 µg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for CCL19/MIP-3 beta at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization Chemotaxis Induced by CCL19/MIP‑3 beta and Neutrali-zation by Human CCL19/MIP‑3 beta Antibody. View Larger

Chemotaxis Induced by CCL19/MIP‑3 beta and Neutrali-zation by Human CCL19/MIP‑3 beta Antibody. Re-combinant Human CCL19/MIP-3 beta (Catalog # 361-MI) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR7 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL19/MIP-3 beta (50 ng/mL) is neutralized (green line) by increasing concentrations of Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361). The ND50 is typically 2-10 µg/mL.

Simple Western Detection of Human CCL19/MIP-3 beta antibody by Simple Western<sup>TM</sup>. View Larger

Detection of Human CCL19/MIP‑3 beta by Simple WesternTM. Simple Western lane view shows conditioned media from monocyte-derived macrophages untreated (-) or treated (+) with 1 µg/mL LPS and 40 ng/mL Recombinant Human IFN-gamma (Catalog # 285-IF) for 24 hours. A specific band was detected for CCL19/MIP-3 beta at approximately 8 kDa (as indicated) using 50 µg/mL of Goat Anti-Human CCL19/MIP-3 beta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF361) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL19/MIP-3 beta

MIP-3 beta, also known as ELC (EBI1-Ligand Chemokine), is one of many novel beta chemokines identified through bioinformatics. MIP-3 beta cDNA encodes a 98 amino acid (aa) residue precursor protein with a predicted 21 aa residue signal peptide that is cleaved to form the 77 aa residue mature secreted protein. MIP-3 beta is distantly related to other beta chemokines (20-30% aa sequence identity) and the gene for MIP-3 beta  has been mapped to chromosome 9p13 rather than chromosome 17 where the genes for many human beta chemokines are clustered. MIP-3 beta has been shown to be constitutively expressed in various lymphoid tissues (including thymus, lymph nodes, appendix and spleen). The expression of MIP-3 beta is down-regulated by the anti-inflammatory cytokine IL-10. MIP-3 beta has been shown to be a unique functional ligand for CCR7 (previously referred to as the Epstein-Barr virus-induced gene 1 (EBI1) orphan receptor), a chemokine receptor that is expressed in various lymphoid tissues and activated B and T lymphocytes. EBI1 is strongly up-regulated in B cells infected with Epstein-Barr virus and T cells infected with herpesvirus 6 or 7.

Entrez Gene IDs
6363 (Human); 24047 (Mouse); 362506 (Rat)
Alternate Names
beta chemokine exodus-3; Beta-chemokine exodus-3; CC chemokine ligand 19; C-C motif chemokine 19; CCL19; chemokine (C-C motif) ligand 19; CKb11; EBI1-ligand chemokine; ELC; ELCMIP-3-beta; Epstein-Barr virus-induced molecule 1 ligand chemokine; exodus-3; Macrophage inflammatory protein 3 beta; macrophage inflammatory protein 3-beta; MGC34433; MIP3 beta; MIP-3 beta; MIP-3b; MIP3BCK beta-11; SCYA19EBI1 ligand chemokine; small inducible cytokine subfamily A (Cys-Cys), member 19; Small-inducible cytokine A19

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Citations for Human CCL19/MIP-3 beta Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

9 Citations: Showing 1 - 9
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  1. Cross-tissue, single-cell stromal atlas identifies shared pathological fibroblast phenotypes in four chronic inflammatory diseases
    Authors: Ilya Korsunsky, Kevin Wei, Mathilde Pohin, Edy Y. Kim, Francesca Barone, Triin Major et al.
    Med (N Y)
  2. CCL19 with CCL21-tail displays enhanced glycosaminoglycan binding with retained chemotactic potency in dendritic cells
    Authors: Astrid S Jørgensen, Pontian E Adogamhe, Julia M Laufer, Daniel F Legler, Christopher T Veldkamp, Mette M Rosenkilde et al.
    Journal of Leukocyte Biology
  3. Age-Related Changes to Human Tear Composition
    Authors: A Micera, A Di Zazzo, G Esposito, R Longo, W Foulsham, R Sacco, R Sgrulletta, S Bonini
    Invest. Ophthalmol. Vis. Sci., 2018-04-01;59(5):2024-2031.
  4. Association of MIF, but not type I interferon-induced chemokines, with increased disease activity in Asian patients with systemic lupus erythematosus
    Sci Rep, 2016-07-25;6(0):29909.
    Species: Human
    Sample Types: Serum
  5. Distinct modulation of chemokine expression patterns in human monocyte-derived dendritic cells by prostaglandin E(2)
    Authors: Markus Bruckner, Denise Dickel, Eva Singer, Daniel F. Legler
    Cellular Immunology
  6. Expression of podoplanin and other lymphatic markers in the human anterior eye segment.
    Authors: Birke K, Lutjen-Drecoll E, Kerjaschki D, Birke MT
    Invest. Ophthalmol. Vis. Sci., 2009-09-08;51(1):344-54.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  7. CXCL12 is essential for migration of activated Langerhans cells from epidermis to dermis.
    Authors: Ouwehand K, Santegoets SJ, Bruynzeel DP, Scheper RJ, de Gruijl TD, Gibbs S
    Eur. J. Immunol., 2008-11-01;38(11):3050-9.
    Species: Human
    Sample Types: Ex Vivo
    Applications: Blocking
  8. Autologous chemotaxis as a mechanism of tumor cell homing to lymphatics via interstitial flow and autocrine CCR7 signaling.
    Authors: Shields JD, Fleury ME, Yong C, Tomei AA, Randolph GJ, Swartz MA
    Cancer Cell, 2007-06-01;11(6):526-38.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  9. The Ccr7 Ligand ELC (Ccl19) Is Transcytosed in High Endothelial Venules and Mediates T Cell Recruitment
    Authors: Espen S. Baekkevold, Takeshi Yamanaka, Roger T. Palframan, Hege S. Carlsen, Finn P. Reinholt, Ulrich H. von Andrian et al.
    J. Exp. Med

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Human CCL19/MIP-3 beta Antibody
By Anonymous on 07/06/2022
Application: Flow Sample Tested: Splenocytes Species: Human

Does not work for flow cytometry. Positive and negative controls are shown.