Human CCL26/Eotaxin-3 Antibody

Name: Human CCL26/Eotaxin-3 Antibody
Brand: R&D Systems
SKU: MAB653
Rating: 5 (1 reviews)

Catalog #: MAB653
2 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB653-SP
MAB653-500
MAB653-100

Detection of Human CCL26/Eotaxin‑3 by Western Blot.

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All CCL26/Eotaxin-3 Antibodies

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Citations (2)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL26/Eotaxin-3 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL26/Eotaxin-3 in ELISAs and Western blots. Does not cross-react with recombinant human (rh) CCL11/Eotaxin, recombinant mouse (rm) CCL11/Eotaxin, rhCCL24/Eotaxin-2, rmCCL24/Eotaxin-2, rhCCL3, rhCCL4, rmCCL3, rmCCL4, rhCCL5, rhCCL13, or rhCCL14.

Source

Monoclonal Mouse IgG₁ Clone # 115002

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL26/Eotaxin-3
Thr24-Leu94
Accession # Q9Y258

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Human CCL26/Eotaxin-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL26/Eotaxin‑3 Biotinylated Antibody (Catalog # BAF346)

Standard: Recombinant Human CCL26/Eotaxin-3 Protein (Catalog # 653-E3)

Neutralization

Measured by its ability to neutralize CCL26/Eotaxin‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND₅₀) is typically 2-8 µg/mL in the presence of 1 µg/mL Recombinant Human CCL26/Eotaxin‑3.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human CCL26/Eotaxin‑3 by Western Blot. Western blot shows lysates of HUVEC human umbilical vein endothelial cells untreated (-) or treated (+) with 100 U/mL Recombinant Human IL-4 (Catalog # 204-IL) for 24 hours. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for CCL26/ Eotaxin-3 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Neutralization

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Chemotaxis Induced by CCL26/Eotaxin‑3 and Neutralization by Human CCL26/Eotaxin‑3 Antibody. Recombinant Human CCL26/Eotaxin-3 (Catalog # 346-E3) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL26/Eotaxin-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Human CCL26/Eotaxin-3 Monoclonal Antibody (Catalog # MAB653). The ND₅₀ is typically 2-8 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL26/Eotaxin-3

CCL26, also known as Eotaxin-3, is a CC chemokine that functions as a chemoattractant for eosinophils and activated T cells through interactions with CCR3.

Entrez Gene IDs

10344 (Human)

Alternate Names

CC chemokine IMAC; C-C motif chemokine 26; CCL26; chemokine (C-C motif) ligand 26; chemokine N1; Eotaxin3; Eotaxin-3; IMAC; Macrophage inflammatory protein 4-alpha; MGC126714; MIP-4a; MIP-4alpha; SCYA26; SCYA26MIP-4-alpha; small inducible cytokine A26; small inducible cytokine subfamily A (Cys-Cys), member 26; Small-inducible cytokine A26; TSC-1Thymic stroma chemokine-1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL26/Eotaxin-3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
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Eotaxins and CCR3 interaction regulates the Th2 environment of cutaneous T-cell lymphoma.
Authors: Miyagaki T, Sugaya M, Fujita H
J. Invest. Dermatol., 2010-05-27;130(9):2304-11.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-P
Rapid chemokine secretion from endothelial cells originates from 2 distinct compartments.
Authors: Oynebraten I, Bakke O, Brandtzaeg P, Johansen FE, Haraldsen G
Blood, 2004-03-25;104(2):314-20.
Species: Human
Sample Types: Cell Culture Supernates, Whole Cells
Applications: ELISA Development, ICC