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Human CCL5/RANTES Quantikine ELISA Kit

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Human CCL5/RANTES ELISA Platelet-Poor Plasma Standard Curve
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Summary Sample Values Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CCL5/RANTES Quantikine ELISA Kit Summary

Assay Length
3.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (100 uL), Serum (100 uL), EDTA Plasma (100 uL), Heparin Plasma (100 uL), Citrate Plasma (100 uL), Urine (100 uL)
Sensitivity
6.6 pg/mL
Assay Range
31.2 - 2,000 pg/mL (Cell Culture Supernates, Serum, EDTA Plasma, Heparin Plasma, Citrate Plasma, Urine)
Specificity
Natural and recombinant human RANTES
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Sample Values

Serum/Platelet-poor Plasma/Urine - Samples from apparently healthy volunteers were evaluated for the presence of human RANTES in this assay. No medical histories were available for the donors used in this study.

Sample TypeMean (pg/mL)% DetectableRange (pg/mL)
Serum (n=11)57,270100%23,117-77,354
Platelet-poor EDTA plasma (n=11)849100%262-1587
Platelet-poor heparin plasma (n=11)1340100%308-3527
Platelet-poor citrate plasma (n=11)467100%202-874
Urine (n=10)65680%ND-1540
ND=Non-detectable

Cell Culture Supernates - Humean peripheral blood mononuclear cells (1x106 cells/mL) were cultured in RPMI supplemented with 10% fetal bovine serum, 50 μM beta -mercaptoethanol, 2 mM L-glutamine, 100 units/mL penicillin, and 100 μg/mL streptomycin sulfate. The cells were cultured stimulated with 10 μg/mL PHA for 1 and 5 days. Aliquots were removed and assayed for levels of human RANTES.

ConditionDay 1 (pg/mL)Day 5 (pg/mL)
Unstimulated cells375667
Stimulated cells 46602830

Note: Sample collection and handling procedures have a significant impact on measured RANTES levels. Refer to the Sample Collection and Storage section for the recommended sample collection procedures. 



Product Summary

The Quantikine Human RANTES Immunoassay is a 3.5 hour solid phase ELISA designed to measure human RANTES levels in cell culture supernates, serum, plasma, and urine. It contains E. coli-derived recombinant human RANTES and antibodies raised against the recombinant factor. This immunoassay has been shown to accurately quantitate the recombinant factor. Results obtained using natural human RANTES showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values of natural human RANTES.

Recovery

The recovery of RANTES spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 101 95-110
Platelet-poor Citrate Plasma (n=4) 99 93-105
Platelet-poor EDTA Plasma (n=4) 98 93-103
Platelet-poor Heparin Plasma (n=4) 99 93-105
Serum (n=5) 101 94-107
Urine (n=10) 102 88-114

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of RANTES were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CCL5/RANTES ELISA Linearity

Scientific Data

N/A Human CCL5/RANTES ELISA Platelet-Poor Plasma Standard Curve View Larger

Human CCL5/RANTES ELISA Platelet-Poor Plasma Standard Curve

N/A Human CCL5/RANTES ELISA Cell Culture Supernates/Serum/Urine Standard Curve View Larger

Human CCL5/RANTES ELISA Cell Culture Supernates/Serum/Urine Standard Curve

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CCL5/RANTES

RANTES (Regulated upon Activation, Normal T cell Expressed and presumably Secreted), also known as CCL5, is a member of the "CC" subfamily of chemokines. It plays a primary role in the inflammatory immune response via its ability to chemoattract leukocytes and modulate their function. The cDNA for RANTES was initially discovered by subtractive hybridization as a T cell specific sequence (1, 2). Human RANTES cDNA encodes a highly basic 91 amino acid (aa) residue precursor polypeptide with a 23 aa hydrophobic signal peptide that is cleaved to generate the 68 aa mature protein (1, 2). Human RANTES exhibits approximately 85% homology with mouse RANTES at the deduced aa level (3, 4). 

RANTES is a potent chemoattractant for a number of different cell types including unstimulated CD4+/CD45RO+ memory T cells and stimulated CD4+ and CD8+ T cells with naive and memory phenotypes, NK cells, basophils, eosinophils, dendritic cells, mast cells, monocytes, and microglia (5-13). In addition to its effects on migration, RANTES can activate a number of cell types including T cells (14-16), monocytes (17), neutrophils (17), NK cells (7), dendritic cells (18), and astrocytes (19). T cell activation generally requires relatively high RANTES concentrations (~ 1 μM) and is dependent upon aggregation of the molecule and association with cell surface glycosaminoglycans (GAGs) (15-17). Whether this activity occurs in vivo remains unclear although in mice, intraperitoneally injected RANTES mutants that are unable to aggregate and/or bind GAG, are not capable of attracting leukocytes when compared to wild-type controls (20). Other in vivo studies show that RANTES knockout mice exhibit deficient recruitment of leukocytes to sites of acute inflammation (21). 
RANTES, is known to interact with four identified seven transmembrane G-protein coupled receptors: CCR1, CCR3, CCR4, and CCR5 (22-25). RANTES stimulation can initiate a variety of signaling cascades that are cell context dependent. For instance, in T-cells, RANTES can stimulate elevations of intracellular Ca2+ (26), and activation of focal adhesion kinase (FAK) (27), protein kinase A (28), PI3-kinase (14), Rho GTPase (29), and JAK/STAT signaling pathways (30). The cytomegalovirus protein US28 exhibits significant homology with CC chemokine receptors and is capable of binding RANTES (31). Membrane-spanning US28 can, depending on the context, signal in a constitutive manner (32), bind RANTES and initiate G-protein-mediated signaling cascades (33), or sequester RANTES and potentially alter inflammatory responses (34-36). 
The RANTES receptor CCR5 is also the primary co-receptor for R5 (M-tropic) variants of HIV-1 (37, 38). It has been demonstrated that RANTES, as well as the other CCR5 ligands, macrophage inflammatory protein (MIP)-1 alpha and MIP-1 beta, can competitively inhibit CCR5/HIV-1 interaction and suppress viral infection in vitro (39, 40). These effects apparently do not require fully intact signaling from the CCR5 receptor (41). Consequently, modified forms of RANTES and non-peptide compounds that block the interaction of HIV-1 with CCR5 show promise for future therapies (41-44). In contrast, several reports show that RANTES can enhance in vitro replication of X4 (T-tropic) variants of HIV-1 that use CXCR4 as a co-receptor rather than CCR5 (45, 46). This activity usually requires relatively high RANTES concentrations (~μM) and is dependent upon interaction with cell surface GAGs, oligomerization, and activation of tyrosine kinase and MAP kinase signaling cascades (46, 47).

Entrez Gene IDs:
6352 (Human); 20304 (Mouse); 403522 (Canine); 493689 (Feline)
Alternate Names:
CCL5; chemokine (C-C motif) ligand 5; D17S136Enormally T-expressed, and presumably secreted; EoCP; Eosinophil chemotactic cytokine; RANTES; SISd; SIS-delta; small inducible cytokine A5 (RANTES); small inducible cytokine subfamily A (Cys-Cys), member 5; Small-inducible cytokine A5; T cell-specific protein P228; T-cell specific protein p288; TCP228T-cell-specific protein RANTES
&#9888; WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Citations for Human CCL5/RANTES Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

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  1. NR1D1 stimulates antitumor immune responses in breast cancer by activating cGAS-STING signaling
    Authors: Ka, NL;Park, MK;Kim, SS;Jeon, Y;Hwang, S;Kim, SM;Lim, GY;Lee, H;Lee, MO;
    Cancer research
    Species: Human
    Sample Types: Cell Culture Supernates
  2. Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
    Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
    PLoS ONE, 2023-04-20;18(4):e0284645.
    Species: Human
    Sample Types: Cell Culture Supernates
  3. Effect of stimulator of interferon genes (STING) signaling on radiation-induced chemokine expression in human osteosarcoma cells
    Authors: SS Withers, CE Moeller, CN Quick, CC Liu, SM Baham, JS Looper, R Subramania, KG Kousoulas
    PLoS ONE, 2023;18(4):e0284645.
    Species: Human
    Sample Types: Cell Culture Supernates
  4. Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
    Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
    Nature Microbiology, 2022-12-15;0(0):.
    Species: Human
    Sample Types: Serum
  5. CCL5 Levels Predict Stroke Volume Growth in Acute Ischemic Stroke and Significantly Diminish in Hemorrhagic Stroke Patients
    Authors: FJ Julián-Vil, M Serrano-Po, E Ramalle-Gó, A Martínez, L Ochoa-Call
    International Journal of Molecular Sciences, 2022-09-01;23(17):.
    Species: Human
    Sample Types: Serum
  6. Serum and Vascular Stiffness Biomarkers Associated with the Severity of Degenerative Aortic Valve Stenosis and Cardiovascular Outcomes
    Authors: J Baran, ? Niewiara, J Podolec, M Siedli?ski, E Józefczuk, A Bernacik, R Badacz, T Przew?ocki, P Pieni??ek, K ?mudka, J Legutko, A Kab?ak-Zie
    Oncogene, 2022-06-17;9(6):.
    Species: Human
    Sample Types: Serum
  7. KIF4A promotes tumor progression of bladder cancer via CXCL5 dependent myeloid-derived suppressor cells recruitment
    Authors: N Lin, L Chen, Y Zhang, Y Yang, L Zhang, L Chen, P Zhang, H Su, M Yin
    Scientific Reports, 2022-04-10;12(1):6015.
    Species: Human
    Sample Types: Cell Culture Supernates
  8. IFI16 inhibits DNA repair that potentiates type-I interferon-induced antitumor effects in triple negative breast cancer
    Authors: NL Ka, GY Lim, S Hwang, SS Kim, MO Lee
    Cell Reports, 2021-12-21;37(12):110138.
    Species: Human
    Sample Types: Cell Culture Supernates
  9. Metabolic modulation by CDK4/6 inhibitor promotes chemokine-mediated recruitment of T�cells into mammary tumors
    Authors: RV Uzhachenko, V Bharti, Z Ouyang, A Blevins, S Mont, N Saleh, HA Lawrence, C Shen, SC Chen, GD Ayers, DG DeNardo, C Arteaga, A Richmond, AE Vilgelm
    Cell Reports, 2021-04-06;35(1):108944.
    Species: Human
    Sample Types: Cell Culture Supernates
  10. NF&kapppaB-Activated COX2/PGE2/EP4 Axis Controls the Magnitude and Selectivity of BCG-Induced Inflammation in Human Bladder Cancer Tissues
    Authors: OM Ibrahim, PH Basse, W Jiang, K Guru, G Chatta, P Kalinski
    Cancers, 2021-03-16;13(6):.
    Species: Human
    Sample Types: Cell Culture Supernates
  11. In Ovarian Cancer Multicellular Spheroids, Platelet Releasate Promotes Growth, Expansion of ALDH+ and CD133+ Cancer Stem Cells, and Protection against the Cytotoxic Effects of Cisplatin, Carboplatin and Paclitaxel
    Authors: N Casagrande, C Borghese, F Agostini, C Durante, M Mazzucato, A Colombatti, D Aldinucci
    International Journal of Molecular Sciences, 2021-03-16;22(6):.
    Species: Human
    Sample Types: Plasma
  12. Tissue-specific endothelial cell heterogeneity contributes to unequal inflammatory responses
    Authors: H Gunawardan, T Romero, N Yao, S Heidt, A Mulder, DA Elashoff, NM Valenzuela
    Scientific Reports, 2021-01-21;11(1):1949.
    Species: Human
    Sample Types: Cell Culture Supernates
  13. Hepatocyte-specific IL11 cis-signaling drives lipotoxicity and underlies the transition from NAFLD to NASH
    Authors: J Dong, S Viswanatha, E Adami, BK Singh, SP Chothani, B Ng, WW Lim, J Zhou, M Tripathi, NSJ Ko, SG Shekeran, J Tan, SY Lim, M Wang, PM Lio, PM Yen, S Schafer, SA Cook, AA Widjaja
    Nature Communications, 2021-01-04;12(1):66.
    Species: Human
    Sample Types: Cell Culture Supernates
  14. Loss of FOXF1 expression promotes human lung-resident mesenchymal stromal cell migration via ATX/LPA/LPA1 signaling axis
    Authors: P Cao, NM Walker, RR Braeuer, S Mazzoni-Pu, Y Aoki, K Misumi, DS Wheeler, R Vittal, VN Lama
    Scientific Reports, 2020-12-04;10(1):21231.
    Species: Human
    Sample Types: Cell Culture Supernates
  15. Proteomics of extracellular vesicles produced by Granulicatella adiacens, which causes infective endocarditis
    Authors: SA Alkandari, RG Bhardwaj, A Ellepola, M Karched
    PLoS ONE, 2020-11-20;15(11):e0227657.
    Species: Human
    Sample Types: Cell Culture Supernates
  16. Preconditioning mesenchymal stromal cells with flagellin enhances the anti?inflammatory ability of their secretome against lipopolysaccharide?induced acute lung injury
    Authors: R Li, Y Li, X Dong
    Molecular Medicine Reports, 2020-07-28;0(0):.
    Species: Human
    Sample Types: BALF
  17. Heatr9 is an infection responsive gene that affects cytokine production in alveolar epithelial cells
    Authors: CJ Stairiker, M van Meurs, LG Leon, AA Brouwers-H, L Rijsbergen, YM Mueller, PD Katsikis
    PLoS ONE, 2020-07-17;15(7):e0236195.
    Species: Human
    Sample Types: Cell Culture Supernates
  18. DNA hypomethylation promotes transposable element expression and activation of immune signaling in renal cell cancer
    Authors: AA de Cubas, W Dunker, A Zaninovich, RA Hongo, A Bhatia, A Panda, KE Beckermann, G Bhanot, S Ganesan, J Karijolich, WK Rathmell
    JCI Insight, 2020-06-04;5(11):.
    Species: Human
    Sample Types: Cell Culture Supernates
  19. Pharmacological inhibition of P2RX7 ameliorates liver injury by reducing inflammation and fibrosis
    Authors: B Baeza-Raja, A Goodyear, X Liu, K Lam, L Yamamoto, Y Li, GS Dodson, T Takeuchi, T Kisseleva, DA Brenner, K Dabbagh
    PLoS ONE, 2020-06-03;15(6):e0234038.
    Species: Human
    Sample Types: Cell Culture Supernates
  20. Diminished hepatic IFN response following HCV clearance triggers HBV reactivation in coinfection
    Authors: X Cheng, T Uchida, Y Xia, R Umarova, CJ Liu, PJ Chen, A Gaggar, V Suri, MM Mücke, J Vermehren, S Zeuzem, Y Teraoka, M Osawa, H Aikata, K Tsuji, N Mori, S Hige, Y Karino, M Imamura, K Chayama, TJ Liang
    J. Clin. Invest., 2020-06-01;0(0):.
    Species: Mouse
    Sample Types: Serum
  21. Plasma RANTES level is correlated with cardio-cerebral atherosclerosis burden in patients with ischemic cerebrovascular disease
    Authors: Q Kong, X Ma, JX Lyu, C Wang, XY Du, YQ Guan
    Chronic Dis Transl Med, 2020-03-10;6(1):46-54.
    Species: Human
    Sample Types: Plasma
  22. Defining the landscape of ATP-competitive inhibitor resistance residues in protein kinases
    Authors: NS Persky, D Hernandez, M Do Carmo, L Brenan, O Cohen, S Kitajima, U Nayar, A Walker, S Pantel, Y Lee, J Cordova, M Sathappa, C Zhu, TK Hayes, P Ram, P Pancholi, TS Mikkelsen, DA Barbie, X Yang, R Haq, F Piccioni, DE Root, CM Johannesse
    Nat. Struct. Mol. Biol., 2020-01-10;27(1):92-104.
    Species: Human
    Sample Types: Cell Culture Supernates
  23. Retinoblastoma inactivation induces a protumoral microenvironment via enhanced CCL2 secretion
    Authors: F Li, S Kitajima, S Kohno, A Yoshida, S Tange, S Sasaki, N Okada, Y Nishimoto, H Muranaka, N Nagatani, M Suzuki, S Masuda, TC Thai, T Nishiuchi, T Tanaka, DA Barbie, N Mukaida, C Takahashi
    Cancer Res., 2019-06-12;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  24. VSIG-3 as a ligand of VISTA inhibits human T-cell function.
    Authors: Wang J, Wu G, Manick B, Hernandez V, Renelt M, Erickson C, Guan J, Singh R, Rollins S, Solorz A, Bi M, Li J, Grabowski D, Dirkx J, Tracy C, Stuart T, Ellinghuysen C, Desmond D, Foster C, Kalabokis V
    Immunology, 2018-10-10;156(1):74-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  25. Suppression of STING associated with LKB1 loss in KRAS-driven lung cancer
    Authors: S Kitajima, E Ivanova, S Guo, R Yoshida, M Campisi, SK Sundararam, S Tange, Y Mitsuishi, TC Thai, S Masuda, BP Piel, LM Sholl, PT Kirschmeie, CP Paweletz, H Watanabe, M Yajima, DA Barbie
    Cancer Discov, 2018-10-08;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  26. Induction of chemokine (C-C motif) ligand 5 by Epstein-Barr virus infection enhances tumor angiogenesis in nasopharyngeal carcinoma
    Authors: W Ma, L Feng, S Zhang, H Zhang, X Zhang, X Qi, Y Zhang, Q Feng, T Xiang, YX Zeng
    Cancer Sci., 2018-04-28;109(5):1710-1722.
    Species: Human
    Sample Types: Cell Culture Supernates
  27. C-C motif ligand 5 promotes migration of prostate cancer cells in the prostate cancer bone metastasis microenvironment
    Authors: S Urata, K Izumi, K Hiratsuka, A Maolake, A Natsagdorj, K Shigehara, H Iwamoto, S Kadomoto, T Makino, R Naito, Y Kadono, WJ Lin, G Wufuer, K Narimoto, A Mizokami
    Cancer Sci., 2018-02-14;109(3):724-731.
    Species: Human
    Sample Types: Cell Culture Supernates
  28. Rapid Generation of Multiple Loci-Engineered Marker-free Poxvirus and Characterization of a Clinical-Grade Oncolytic Vaccinia Virus
    Authors: ZS Guo, Z Liu, M Sathaiah, J Wang, R Ravindrana, E Kim, S Huang, TW Kenniston, JC Bell, HJ Zeh, LH Butterfiel, A Gambotto, DL Bartlett
    Mol Ther Methods Clin Dev, 2017-09-30;7(0):112-122.
    Species: Human
    Sample Types: Cell Culture Supernates
  29. Downregulation of tumor suppressive microRNAs in vivo in dense breast tissue of postmenopausal women
    Authors: A Abrahamsso, A Capodanno, A Rzepecka, C Dabrosin
    Oncotarget, 2017-09-15;8(54):92134-92142.
    Species: Human
    Sample Types: Cell Culture Supernates
  30. Aberrant monocyte responses predict and characterize dengue virus infection in individuals with severe disease
    Authors: YK Yong, HY Tan, SH Jen, EM Shankar, SK Natkunam, J Sathar, R Manikam, SD Sekaran
    J Transl Med, 2017-05-31;15(1):121.
    Species: Human
    Sample Types: Plasma
  31. Prostate cancer downregulated SIRP-? modulates apoptosis and proliferation through p38-MAPK/NF-?B/COX-2 signaling
    Authors: C Yao, G Li, M Cai, Y Qian, L Wang, L Xiao, F Thaiss, B Shi
    Oncol Lett, 2017-04-21;13(6):4995-5001.
    Species: Human
    Sample Types: Cell Culture Supernates, Serum
  32. TWEAK/Fn14 promotes pro-inflammatory cytokine secretion in hepatic stellate cells via NF-?B/STAT3 pathways
    Authors: A Wang, F Zhang, H Xu, M Xu, Y Cao, C Wang, Y Xu, M Su, M Zhang, Y Zhuge
    Mol. Immunol., 2017-04-12;87(0):67-75.
    Species: Human
    Sample Types: Cell Culture Supernates
  33. CD8+ T cells promote proliferation of benign prostatic hyperplasia epithelial cells under low androgen level via modulation of CCL5/STAT5/CCND1 signaling pathway
    Authors: Y Yang, S Hu, J Liu, Y Cui, Y Fan, T Lv, L Liu, J Li, Q He, W Han, W Yu, Y Sun, J Jin
    Sci Rep, 2017-02-20;7(0):42893.
    Species: Human
    Sample Types: Cell Culture Supernates
  34. Tumor necrosis factor alpha inhibitors have no effect on a human T-lymphotropic virus type-I (HTLV-I)-infected cell line from patients with HTLV-I-associated myelopathy
    Authors: S Fukui, H Nakamura, Y Takahashi, N Iwamoto, H Hasegawa, K Yanagihara, T Nakamura, A Okayama, A Kawakami
    BMC Immunol, 2017-02-03;18(1):7.
    Species: Human
    Sample Types: Cell Culture Supernates
  35. -2518 A/G MCP-1 but not -403 G/A RANTES gene polymorphism is associated with enhanced risk of basal cell carcinoma
    Authors: Roman Nowicki
    Postepy Dermatol Alergol, 2016-10-21;33(5):381-385.
    Species: Human
    Sample Types: Serum
  36. Stability of cytokines, chemokines and soluble activation markers in unprocessed blood stored under different conditions
    Cytokine, 2016-05-18;84(0):17-24.
    Species: Human
    Sample Types: Plasma
  37. Loss of intraepidermal nerve fiber density during SIV peripheral neuropathy is mediated by monocyte activation and elevated monocyte chemotactic proteins.
    Authors: Lakritz J, Robinson J, Polydefkis M, Miller A, Burdo T
    J Neuroinflammation, 2015-12-18;12(0):237.
    Species: Primate
    Sample Types: Plasma
  38. Hypoxic Preconditioning Increases Survival and Pro-Angiogenic Capacity of Human Cord Blood Mesenchymal Stromal Cells In Vitro.
    Authors: Bader A, Klose K, Bieback K, Korinth D, Schneider M, Seifert M, Choi Y, Kurtz A, Falk V, Stamm C
    PLoS ONE, 2015-09-18;10(9):e0138477.
    Species: Human
    Sample Types: Cell Culture Supernates
  39. Serum levels of chemokines CCL4 and CCL5 in cirrhotic patients indicate the presence of hepatocellular carcinoma.
    Authors: Sadeghi M, Lahdou I, Oweira H, Daniel V, Terness P, Schmidt J, Weiss K, Longerich T, Schemmer P, Opelz G, Mehrabi A
    Br J Cancer, 2015-08-13;113(5):756-62.
    Species: Human
    Sample Types: Serum
  40. The TNF Family Molecules LIGHT and Lymphotoxin alphabeta Induce a Distinct Steroid-Resistant Inflammatory Phenotype in Human Lung Epithelial Cells.
    Authors: da Silva Antunes R, Madge L, Soroosh P, Tocker J, Croft M
    J Immunol, 2015-07-24;195(5):2429-41.
    Species: Human
    Sample Types: Cell Culture Supernates
  41. Nuclear NF-kappaB p65 in peripheral blood mononuclear cells correlates with urinary MCP-1, RANTES and the severity of type 2 diabetic nephropathy.
    Authors: Yi B, Hu X, Zhang H, Huang J, Liu J, Hu J, Li W, Huang L
    PLoS ONE, 2014-06-17;9(6):e99633.
    Species: Human
    Sample Types: Urine
  42. IL-10 up-regulates CCL5 expression in vascular smooth muscle cells from spontaneously hypertensive rats.
    Authors: Kim H, Kim H
    Cytokine, 2014-03-19;68(1):40-9.
    Species: Rat
    Sample Types: Cell Culture Supernates
  43. Chlorogenic acid inhibits human platelet activation and thrombus formation.
    Authors: Fuentes E, Caballero J, Alarcon M, Rojas A, Palomo I
    PLoS ONE, 2014-03-05;9(3):e90699.
    Species: Human
    Sample Types: Cell Culture Supernates
  44. Hydroxycarbamide reduces eosinophil adhesion and degranulation in sickle cell anaemia patients.
    Authors: Pallis F, Conran N, Fertrin K, Olalla Saad S, Costa F, Franco-Penteado C
    Br J Haematol, 2013-10-28;164(2):286-95.
    Species: Human
    Sample Types: Serum
  45. Decreased plasma cytokines are associated with low platelet counts in aplastic anemia and immune thrombocytopenic purpura.
    J. Thromb. Haemost., 2012-08-01;10(8):1616-23.
    Species: Human
    Sample Types: Serum
  46. Regulation of inflammatory chemokine receptors on blood T cells associated to the circulating versus liver chemokines in dengue Fever.
    Authors: de-Oliveira-Pinto LM, Marinho CF, Povoa TF, de Azeredo EL, de Souza LA, Barbosa LD, Motta-Castro AR, Alves AM, Avila CA, de Souza LJ, da Cunha RV, Damasco PV, Paes MV, Kubelka CF
    PLoS ONE, 2012-07-16;7(7):e38527.
    Species: Human
    Sample Types: Plasma
  47. Multiple recognition assay reveals prostasomes as promising plasma biomarkers for prostate cancer.
    Authors: Tavoosidana G, Ronquist G, Darmanis S, Yan J, Carlsson L, Wu D, Conze T, Ek P, Semjonow A, Eltze E, Larsson A, Landegren UD, Kamali-Moghaddam M
    Proc. Natl. Acad. Sci. U.S.A., 2011-05-09;108(21):8809-14.
    Species: Human
    Sample Types: Platelet Poor Plasma
  48. Persistently raised plasma levels of platelet-derived inflammatory mediators in HIV-infected patients during highly active anti-retroviral therapy.
    Authors: Landro L, Ueland T, Otterdal K, Froland SS, Aukrust P
    J. Thromb. Haemost., 2011-05-01;9(5):1075-7.
    Species: Human
    Sample Types: Platelet Poor Plasma
  49. Phagocytosis of apoptotic cells modulates mesenchymal stem cells osteogenic differentiation to enhance IL-17 and RANKL expression on CD4+ T cells.
    Authors: Tso GH, Law HK, Tu W
    Stem Cells, 2010-05-01;28(5):939-54.
    Species: Human
    Sample Types: Cell Culture Supernates
  50. Human CD4- 8- T cells are a distinctive immunoregulatory subset.
    Authors: Huang MC, Patel K, Taub DD, Longo DL, Goetzl EJ
    FASEB J, 2010-02-12;24(7):2558-66.
    Species: Human
    Sample Types: Cell Culture Supernates
  51. A phase I trial of bortezomib with temozolomide in patients with advanced melanoma: toxicities, antitumor effects, and modulation of therapeutic targets.
    Authors: Su Y, Amiri KI, Horton LW
    Clin. Cancer Res., 2009-12-22;16(1):348-57.
    Species: Human
    Sample Types: Serum
  52. Tumour necrosis factor alpha stimulates the production of monocyte chemoattractants by extravillous trophoblast cells via differential activation of MAPK pathways.
    Authors: Renaud SJ, Sullivan R, Graham CH
    Placenta, 2009-02-08;30(4):313-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  53. HLA-DR+ leukocytes acquire CD1 antigens in embryonic and fetal human skin and contain functional antigen-presenting cells.
    Authors: Schuster C, Vaculik C, Fiala C, Meindl S, Brandt O, Imhof M, Stingl G, Eppel W, Elbe-Burger A
    J. Exp. Med., 2009-01-12;206(1):169-81.
    Species: Human
    Sample Types: Cell Culture Supernates
  54. Coevolution of prostate cancer and bone stroma in three-dimensional coculture: implications for cancer growth and metastasis.
    Authors: Sung SY, Hsieh CL, Law A, Zhau HE, Pathak S, Multani AS, Lim S, Coleman IM, Wu LC, Figg WD, Dahut WL, Nelson P, Lee JK, Amin MB, Lyles R, Johnstone PA, Marshall FF, Chung LW
    Cancer Res., 2008-12-01;68(23):9996-10003.
    Species: Human
    Sample Types: Serum
  55. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
    Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
    Biochem. Biophys. Res. Commun., 2008-11-11;378(1):90-4.
    Species: Human
    Sample Types: Cell Culture Supernates
  56. NF-kappaB modulates sensitivity to apoptosis, proinflammatory and migratory potential in short- versus long-term cultured human gammadelta lymphocytes.
    Authors: Ferrarini M, Delfanti F, Gianolini M, Rizzi C, Alfano M, Lazzarin A, Biswas P
    J. Immunol., 2008-11-01;181(9):5857-64.
    Species: Human
    Sample Types: Cell Culture Supernates
  57. RANTES in exhaled breath condensate of stable and unstable asthma patients.
    Authors: Zietkowski Z, Tomasiak MM, Skiepko R, Bodzenta-Lukaszyk A
    Respir Med, 2008-07-07;102(8):1198-202.
    Species: Human
    Sample Types: Exhaled Breath Condensate (EBC)
  58. Chemokine production and chemokine receptor expression by human glioma cells: role of CXCL10 in tumour cell proliferation.
    Authors: Maru SV, Holloway KA, Flynn G, Lancashire CL, Loughlin AJ, Male DK, Romero IA
    J. Neuroimmunol., 2008-06-09;199(1):35-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  59. In vitro induction of a dendritic cell phenotype in primary human acute myelogenous leukemia (AML) blasts alters the chemokine release profile and increases the levels of T cell chemotactic CCL17 and CCL22.
    Authors: Olsnes AM, Ryningen A, Ersvaer E, Bruserud Ø
    J. Interferon Cytokine Res., 2008-05-01;28(5):297-310.
    Species: Human
    Sample Types: Cell Culture Supernates
  60. Guggulsterone blocks IL-1beta-mediated inflammatory responses by suppressing NF-kappaB activation in fibroblast-like synoviocytes.
    Authors: Lee YR, Lee JH, Noh EM, Kim EK, Song MY, Jung WS, Park SJ, Kim JS, Park JW, Kwon KB, Park BH
    Life Sci., 2008-04-20;82(23):1203-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  61. CT20126, a novel immunosuppressant, prevents collagen-induced arthritis through the down-regulation of inflammatory gene expression by inhibiting NF-kappaB activation.
    Authors: Lee SJ, Nam WD, Na HJ, Cho YL, Ha KS, Hwang JY, Lee H, Kim SO, Kwon YG, Kim YM
    Biochem. Pharmacol., 2008-04-18;76(1):79-90.
    Species: Human
    Sample Types: Cell Culture Supernates
  62. Differential chemokine expression in tubular cells in response to urinary proteins from patients with nephrotic syndrome.
    Authors: Huang Z, Wen Q, Zhou SF, Yu XQ
    Cytokine, 2008-03-24;42(2):222-33.
    Species: Human
    Sample Types: Urine
  63. Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
    Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
    Int. J. Cancer, 2008-02-15;122(4):769-76.
    Species: Human
    Sample Types: Cell Culture Supernates
  64. Proteomics analysis of Hodgkin lymphoma: identification of new players involved in the cross-talk between HRS cells and infiltrating lymphocytes.
    Authors: Ma Y, Visser L, Roelofsen H, de Vries M, Diepstra A, van Imhoff G, van der Wal T, Luinge M, Alvarez-Llamas G, Vos H, Poppema S, Vonk R, Van Den Berg A
    Blood, 2007-12-10;111(4):2339-46.
    Species: Human
    Sample Types: Cell Culture Supernates
  65. Circulating interleukin-15 and RANTES chemokine in Parkinson's disease.
    Authors: Rentzos M, Nikolaou C, Andreadou E, Paraskevas GP, Rombos A, Zoga M, Tsoutsou A, Boufidou F, Kapaki E, Vassilopoulos D
    Acta Neurol. Scand., 2007-12-01;116(6):374-9.
    Species: Human
    Sample Types: Serum
  66. Computed tomographic scan-diagnosed chronic obstructive pulmonary disease-emphysema: eotaxin-1 is associated with bronchodilator response and extent of emphysema.
    Authors: Miller M, Ramsdell J, Friedman PJ, Cho JY, Renvall M, Broide DH
    J. Allergy Clin. Immunol., 2007-11-01;120(5):1118-25.
    Species: Human
    Sample Types: BALF
  67. Functional diversity of T-cell subpopulations in subacute and chronic hypersensitivity pneumonitis.
    Authors: Barrera L, Mendoza F, Zuniga J, Estrada A, Zamora AC, Melendro EI, Ramirez R, Pardo A, Selman M
    Am. J. Respir. Crit. Care Med., 2007-10-18;177(1):44-55.
    Species: Human
    Sample Types: BALF
  68. CXCR3 and CCR5 chemokines in induced sputum from patients with COPD.
    Authors: Costa C, Rufino R, Traves SL, Lapa E Silva JR, Barnes PJ, Donnelly LE
    Chest, 2007-10-09;133(1):26-33.
    Species: Human
    Sample Types: Saliva
  69. Mesenchymal stem cells within tumour stroma promote breast cancer metastasis.
    Authors: Karnoub AE, Dash AB, Vo AP, Sullivan A, Brooks MW, Bell GW, Richardson AL, Polyak K, Tubo R, Weinberg RA
    Nature, 2007-10-04;449(7162):557-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  70. Endothelial damage from cytomegalovirus-specific host immune response can be prevented by targeted disruption of fractalkine-CX3CR1 interaction.
    Authors: Bolovan-Fritts CA, Spector SA
    Blood, 2007-09-25;111(1):175-82.
    Species: Human
    Sample Types: Cell Culture Supernates
  71. Relationship between eosinophilia and levels of chemokines (CCL5 and CCL11) and IL-5 in bronchoalveolar lavage fluid of patients with mustard gas-induced pulmonary fibrosis.
    Authors: Emad A, Emad Y
    J. Clin. Immunol., 2007-07-10;27(6):605-12.
    Species: Human
    Sample Types: BALF
  72. Down-regulation of E-cadherin in human bronchial epithelial cells leads to epidermal growth factor receptor-dependent Th2 cell-promoting activity.
    Authors: Heijink IH, Kies PM, Kauffman HF, Postma DS, van Oosterhout AJ, Vellenga E
    J. Immunol., 2007-06-15;178(12):7678-85.
    Species: Human
    Sample Types: Cell Culture Supernates
  73. High T-cell response to human cytomegalovirus induces chemokine-mediated endothelial cell damage.
    Authors: Bolovan-Fritts CA, Trout RN, Spector SA
    Blood, 2007-05-22;110(6):1857-63.
    Species: Human
    Sample Types: Cell Culture Supernates
  74. An inflammation-induced mechanism for leukocyte transmigration across lymphatic vessel endothelium.
    Authors: Johnson LA, Clasper S, Holt AP, Lalor PF, Baban D, Jackson DG
    J. Exp. Med., 2006-11-20;203(12):2763-77.
    Species: Human
    Sample Types: Cell Culture Supernates
  75. Increased levels of neutrophil-activating peptide-2 in acute coronary syndromes: possible role of platelet-mediated vascular inflammation.
    Authors: Smith C, Damas JK, Otterdal K, ØIe E, Sandberg WJ, Yndestad A, Waehre T, Scholz H, Endresen K, Olofsson PS, Halvorsen B, Gullestad L, Froland SS, Hansson GK, Aukrust P
    J. Am. Coll. Cardiol., 2006-09-27;48(8):1591-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  76. Regulated upon activation, normal T cell expressed and secreted (RANTES) and monocyte chemotactic protein 1 in follicular fluid accumulate differentially in patients with and without endometriosis undergoing in vitro fertilization.
    Authors: Schultze-Mosgau A, Agic A, Diedrich K, Hornung D
    Fertil. Steril., 2006-09-25;86(6):1616-20.
    Species: Human
    Sample Types: Serum
  77. Interaction between the CCR5 chemokine receptors and microbial HSP70.
    Authors: Whittall T, Wang Y, Younson J, Kelly C, Bergmeier L, Peters B, Singh M, Lehner T
    Eur. J. Immunol., 2006-09-01;36(9):2304-14.
    Species: Human
    Sample Types: Cell Culture Supernates
  78. RANTES and monocyte chemoattractant protein 1 as sensitive markers of disease activity in patients with juvenile rheumatoid arthritis: a six-year longitudinal study.
    Authors: Yao TC, Kuo ML, See LC, Ou LS, Lee WI, Chan CK, Huang JL
    Arthritis Rheum., 2006-08-01;54(8):2585-93.
    Species: Human
    Sample Types: Serum
  79. Human Vgamma2Vdelta2 T cells contain cytoplasmic RANTES.
    Authors: Tikhonov I, Deetz CO, Paca R, Berg S, Lukyanenko V, Lim JK, Pauza CD
    Int. Immunol., 2006-06-01;18(8):1243-51.
    Species: Human
    Sample Types: Cell Culture Supernates
  80. Mast cell beta-tryptase selectively cleaves eotaxin and RANTES and abrogates their eosinophil chemotactic activities.
    Authors: Pang L, Nie M, Corbett L, Sutcliffe A, Knox AJ
    J. Immunol., 2006-03-15;176(6):3788-95.
    Species: Human
    Sample Types: Cell Culture Supernates
  81. Increased ENA-78 in the follicular fluid of patients with endometriosis.
    Authors: Wunder DM, Mueller MD, Birkhauser MH, Bersinger NA
    Acta Obstet Gynecol Scand, 2006-01-01;85(3):336-42.
    Species: Human
    Sample Types: Follicular Fluid
  82. Airway inflammation during stable and acutely exacerbated chronic obstructive pulmonary disease.
    Authors: Fujimoto K, Yasuo M, Urushibata K, Hanaoka M, Koizumi T, Kubo K
    Eur. Respir. J., 2005-04-01;25(4):640-6.
    Species: Human
    Sample Types: Sputum
  83. Th2 predominance and CD8+ memory T cell depletion in patients with severe acute respiratory syndrome.
    Authors: Huang JL, Huang J, Duan ZH, Wei J, Min J, Luo XH, Li JG, Tan WP, Wu LZ, Liu RY, Li Y, Shao J, Huang BJ, Zeng YX, Huang W
    Microbes Infect., 2005-02-24;7(3):427-36.
    Species: Human
    Sample Types: Serum
  84. CXCR3 and CCR5 positive T-cell recruitment in acute human renal allograft rejection.
    Authors: Panzer U, Reinking RR, Steinmetz OM, Zahner G, Sudbeck U, Fehr S, Pfalzer B, Schneider A, Thaiss F, Mack M, Conrad S, Huland H, Helmchen U, Stahl RA
    Transplantation, 2004-11-15;78(9):1341-50.
    Species: Human
    Sample Types: Urine
  85. Correlations between IL-4, IL-12 levels and CCL2, CCL5 levels in serum and cerebrospinal fluid of multiple sclerosis patients.
    Authors: Bartosik-Psujek H, Stelmasiak Z
    J Neural Transm, 2004-10-12;112(6):797-803.
    Species: Human
    Sample Types: Serum
  86. Inhibition of human immunodeficiency virus type 1 replication by Z-100, an immunomodulator extracted from human-type tubercle bacilli, in macrophages.
    Authors: Emori Y, Ikeda T, Ohashi T, Masuda T, Kurimoto T, Takei M, Kannagi M
    J. Gen. Virol., 2004-09-01;85(0):2603-13.
    Species: Human
    Sample Types: Cell Culture Supernates
  87. Enhanced monocyte chemoattractant protein-3/CC chemokine ligand-7 in usual interstitial pneumonia.
    Authors: Choi ES, Jakubzick C, Carpenter KJ, Kunkel SL, Evanoff H, Martinez FJ, Flaherty KR, Toews GB, Colby TV, Kazerooni EA, Gross BH, Travis WD, Hogaboam CM
    Am. J. Respir. Crit. Care Med., 2004-06-10;170(5):508-15.
    Species: Human
    Sample Types: Cell Culture Supernates
  88. Lack of phenotypic and functional impairment in dendritic cells from chimpanzees chronically infected with hepatitis C virus.
    Authors: Larsson M, Babcock E, Grakoui A, Shoukry N, Lauer G, Rice C, Walker C, Bhardwaj N
    J. Virol., 2004-06-01;78(12):6151-61.
    Species: Primate - Pan troglodytes (Chimpanzee)
    Sample Types: Cell Culture Supernates
  89. Natural killer T cells infiltrate neuroblastomas expressing the chemokine CCL2.
    Authors: Metelitsa LS, Wu HW, Wang H, Yang Y, Warsi Z, Asgharzadeh S, Groshen S, Wilson SB, Seeger RC
    J. Exp. Med., 2004-05-03;199(9):1213-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  90. Impact of NNRTI compared to PI-based highly active antiretroviral therapy on CCR5 receptor expression, beta-chemokines and IL-16 secretion in HIV-1 infection.
    Authors: Burton CT, »V6þÌ™+ìZ, Hardy GA, Sullivan AK, Nelson MR, Gazzard B, Gotch FM, Imami N
    2002-11-01;130(2):286-92.
    Species: Human
    Sample Types: Plasma
  91. Endogenously expressed nef uncouples cytokine and chemokine production from membrane phenotypic maturation in dendritic cells.
    Authors: Messmer D, Jacque JM, Santisteban C, Bristow C, Han SY, Villamide-Herrera L, Mehlhop E, Marx PA, Steinman RM, Gettie A, Pope M
    J. Immunol., 2002-10-15;169(8):4172-82.
    Species: Human, Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  92. CCR5-binding chemokines modulate CXCL12 (SDF-1)-induced responses of progenitor B cells in human bone marrow through heterologous desensitization of the CXCR4 chemokine receptor.
    Authors: Honczarenko M, Le Y, Glodek AM, Silberstein LE
    Blood, 2002-10-01;100(7):2321-9.
    Species: Human
    Sample Types: Cell Culture Supernates
  93. TNFalpha-induced macrophage chemokine secretion is more dependent on NF-kappaB expression than lipopolysaccharides-induced macrophage chemokine secretion.
    Authors: Ciesielski CJ, Andreakos E, Foxwell BM, Feldmann M
    Eur. J. Immunol., 2002-07-01;32(7):2037-45.
    Species: Human
    Sample Types: Cell Culture Supernates
  94. Analysis of chemokines and chemokine receptor expression in ovarian cancer ascites.
    Authors: Milliken D, Scotton C, Raju S, Balkwill F, Wilson J
    Clin. Cancer Res., 2002-04-01;8(4):1108-14.
    Species: Human
    Sample Types: Serum
  95. Simian immunodeficiency viruses with defective nef genes show increased susceptibility to the noncytotoxic antiviral activity of CD8+ lymphocytes.
    Authors: Binninger-Schinzel D, 2019, Norley S, Adler HS, Oberg HH, Kurth R
    2002-03-01;294(1):209-21.
    Species: Human
    Sample Types: Cell Culture Supernates
  96. Bleomycin stimulates lung fibroblast and epithelial cell lines to release eosinophil chemotactic activity.
    Authors: Sato E, Koyama S
    Eur. Respir. J., 2000-11-01;16(5):951-8.
    Species: Human
    Sample Types: Cell Culture Supernates
  97. Immunization with live attenuated simian immunodeficiency virus induces strong type 1 T helper responses and beta-chemokine production.
    Authors: Gauduin MC, Glickman RL, Ahmad S, Yilma T, Johnson RP
    Proc. Natl. Acad. Sci. U.S.A., 1999-11-23;96(24):14031-6.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates
  98. Protective mucosal immunity elicited by targeted iliac lymph node immunization with a subunit SIV envelope and core vaccine in macaques.
    Authors: Lehner T, —îÊëÖ“§, Wang Y, r¹oŽ&A½ýx, Cranage M, Ïï§Ù´âoÑ‘, Bergmeier LA, Mitchell E, Tao L, Hall G, Dennis M, Cook N, Brookes R, Klavinskis L, Jones I, Doyle C, Ward R
    1996-07-01;2(7):767-75.
    Species: Primate - Macaca mulatta (Rhesus Macaque)
    Sample Types: Cell Culture Supernates

FAQs

  1. Can plasma be used as a sample in Human CCL5/RANTES Quantikine® ELISA kit (Catalog # DRN00B) if platelet-poor plasma is not available?  

    • Platelet-poor plasma is the only type of plasma sample validated for Human CCL5/RANTES Quantikine® ELISA kit. CCL5/RANTES is present in platelet granules and is released upon platelet activation. 

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Human CCL5/RANTES Quantikine ELISA Kit
By Mitch Rechtzigel on 04/24/2023
Sample Tested: Cell culture supernatant
Human CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 11/07/2022
Sample Tested: Serum
Human CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 04/26/2022
Sample Tested: Urine

Measured urine at 2-fold dilution and plasma at 4-fold dilution from healthy controls & patients with disease. All urine samples were below the standard curve. Plasma samples were mostly above the standard curve and would need further dilution.
***Bio-Techne Response: Thank you for reviewing our product. We are sorry to hear that this product did not perform as expected. We have been in touch with the customer to resolve this issue according to our Product Guarantee and to the customer’s satisfaction.***

Human CCL5/RANTES Quantikine ELISA Kit
By Anonymous on 03/21/2019
Sample Tested: Brain Microvascular Endothelial Cells
Human CCL5/RANTES Quantikine ELISA Kit
By Balaji Mahender on 01/09/2018
Sample Tested: Citrate Plasma,EDTA Plasma,Heparin Plasma