Human CCL7/MCP-3/MARC Antibody

Catalog #: MAB282
4 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB282-SP
MAB282-500
MAB282-100

Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody.

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Citations (4)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL7/MCP-3/MARC Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL7/MCP-3/MARC in ELISAs and Western blots. In ELISAs, no cross-reactivity with recombinant human (rh) CCL2/MCP-1, rhCCL8/MCP‑2, or rhCCL13/MCP‑4 is observed.

Source

Monoclonal Mouse IgG₁ Clone # 36320

Purification

Protein A or G purified from ascites

Immunogen

E. coli-derived recombinant human CCL7/MCP-3/MARC
Gln34-109
Accession # Q7Z7Q8

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CCL7/MCP‑3/MARC (Catalog # 282-P3)

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Intracellular Staining by Flow Cytometry

2.5 µg/10⁶ cells

THP‑1 human acute monocytic leukemia cell line fixed with paraformaldehyde and permeabilized with saponin

Human CCL7/MCP-3 Sandwich Immunoassay

Recommended Concentration

Reagent

ELISA Capture (Matched Antibody Pair)

2-8 µg/mL

Use in combination with:

Detection Reagent: Human CCL7/MCP‑3/MARC Biotinylated Antibody (Catalog # BAF282)

Standard: Recombinant Human CCL7/MCP-3 Protein (Catalog # 282-P3)

Neutralization

Measured by its ability to neutralize CCL7/MCP‑3/MARC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 1.5-4.5 µg/mL in the presence of 1 µg/mL Recombinant Human CCL7/MCP‑3/MARC.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL7/MCP‑3 and Neutralization by Human CCL7/MCP‑3 Antibody. Recombinant Human CCL7/MCP-3 (Catalog # 282-P3) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL7/MCP-3 (1 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL7/MCP-3 Monoclonal Antibody (Catalog # MAB282). The ND₅₀ is typically 1.5-4.5 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL7/MCP-3/MARC

MCP-2 and CCL7 are two monocyte chemotactic proteins produced by human MG-63 osteosarcoma cells. Both MCP-2 and CCL7 are members of the C-C family of chemokines and share 62% and 71% amino acid sequence identity, respectively, with MCP-1. CCL7 also shares 58% amino acid identity with MCP-2.

CCL7 cDNA encodes a 99 amino acid residue precursor protein from which the N-terminal 23 amino acid residues are cleaved to generate the 76 amino acid residue mature CCL7. Mature CCL7 contains a potential N-linked and several possible O-linked glycosylation sites.

Similarly to other C-C chemokines, all three MCP proteins are monocyte chemoattractants. In addition, the three MCPs can chemoattract activated NK cells as well as CD4⁺ and CD8⁺ T lymphocytes. All three cytokines have also been shown to attract eosinophils and induce histamine secretion from basophils.

Entrez Gene IDs

6354 (Human); 20306 (Mouse)

Alternate Names

C-C motif chemokine 7; CCL7; chemokine (C-C motif) ligand 7; FIC; MARC; MCP-3; MCP3Monocyte chemotactic protein 3; MCP-3Small-inducible cytokine A7; MGC138465; Monocyte chemoattractant protein 3; NC28SCYA6; SCYA7MGC138463; small inducible cytokine A7 (monocyte chemotactic protein 3)

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCL7/MCP-3/MARC Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Chemokine positioning determines mutually exclusive roles for their receptors in extravasation of pathogenic human T cells
Authors: F Parween, SP Singh, HH Zhang, N Kathuria, FA Otaizo-Car, A Shamsaddin, PJ Gardina, S Ganesan, J Kabat, HA Lorenzi, TG Myers, JM Farber
bioRxiv : the preprint server for biology, 2023-02-13;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Tumoral NOX4 recruits M2 tumor-associated macrophages via ROS/PI3K signaling-dependent various cytokine production to promote NSCLC growth
Authors: J Zhang, H Li, Q Wu, Y Chen, Y Deng, Z Yang, L Zhang, B Liu
Redox Biol, 2019-02-06;22(0):101116.
Species: Human
Sample Types: Cell Culture Supernates
Applications: Neutralization
Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
Int. J. Cancer, 2010-07-15;127(2):332-44.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: IHC-P, Neutralization
Chemokine and chemokine receptor expression in paired peripheral blood mononuclear cells and synovial tissue of patients with rheumatoid arthritis, osteoarthritis, and reactive arthritis.
Authors: Haringman JJ, Smeets TJ, Reinders-Blankert P
Ann. Rheum. Dis., 2005-08-17;65(3):294-300.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr