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Human CCR3 Antibody

Catalog #: MAB155
20 Citations 1 Review Datasheet / COA / SDS
Clone 61828 was used by HLDA to establish CD designation
Catalog # Availability Size / Price Qty
MAB155-100
MAB155-SP
CCR3 in Human Lymph Node.
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Product Details
Citations (20)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR3 Antibody Summary

Species Reactivity
Human
Specificity
Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.
Source
Monoclonal Rat IgG2A Clone # 61828
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.

Applications

Recommended Concentration
Sample
Flow Cytometry
2.5 µg/106 cells
Human CD49d+ peripheral blood cells
Immunohistochemistry
8-25 µg/mL
See below
CyTOF-ready
Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Neutralization
Measured by its ability to neutralize CCL11/Eotaxin-induced chemotaxis in the BaF3 mouse proB cell line transfected with human CCR3. The Neutralization Dose (ND50) is typically 0.05-0.25 µg/mL in the presence of 0.333 µg/mL Recombinant Human CCL11/Eotaxin.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunohistochemistry CCR3 antibody in Human Lymph Node by Immunohistochemistry (IHC-P). View Larger

CCR3 in Human Lymph Node. CCR3 was detected in immersion fixed paraffin-embedded sections of human lymph node using 15 µg/mL Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155) overnight at 4 °C. Tissue was stained with the Anti-Rat HRP-AEC Cell & Tissue Staining Kit (red; Catalog # CTS018) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Neutralization Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody. View Larger

Chemotaxis Induced by CCL11/Eotaxin and Neutralization by Human CCR3 Antibody. Recombinant Human CCL11/Eotaxin (Catalog # 320-EO) chemoattracts the BaF3 mouse proB cell line transfected with human CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant CCL11/Eotaxin (0.333 µg/mL) is neutralized (green line) by increasing concentrations of Rat Anti-Human CCR3 Monoclonal Antibody (Catalog # MAB155). The ND50 is typically 0.05-0.25 µg/mL.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs
1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)
Alternate Names
b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841

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Citations for Human CCR3 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

20 Citations: Showing 1 - 10
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  1. CCR3 blockage elicits polyploidization associated with the signatures of epithelial-mesenchymal transition in carcinoma cell lines
    Authors: Y Kaibori, D Nagakubo
    Cancer Gene Therapy, 2022-09-19;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: ICC
  2. Blockade of Autocrine CCL5 Responses Inhibits Zika Virus Persistence and Spread in Human Brain Microvascular Endothelial Cells
    Authors: MC Mladinich, JN Conde, WR Schutt, SY Sohn, ER Mackow
    MBio, 2021-08-17;12(4):e0196221.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  3. A blocking monoclonal antibody to CCL24 alleviates liver fibrosis and inflammation in experimental models of liver damage
    Authors: Michal Segal-Salto, Neta Barashi, Avi Katav, Vicktoria Edelshtein, Arnon Aharon, Sharon Hashmueli et al.
    JHEP Reports
  4. Evidence for chemokine synergy during neutrophil migration in ARDS
    Thorax, 2016-08-05;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Hypoxia induced CCL28 promotes angiogenesis in lung adenocarcinoma by targeting CCR3 on endothelial cells
    Authors: Guichun Huang
    Sci Rep, 2016-06-02;6(0):27152.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  6. Expression and prognostic significance of CCL11/CCR3 in glioblastoma
    Oncotarget, 2016-05-31;7(22):32617-27.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  7. Tumour hypoxia promotes tolerance and angiogenesis via CCL28 and T(reg) cells.
    Authors: Facciabene A, Peng X, Hagemann IS, Balint K, Barchetti A, Wang LP, Gimotty PA, Gilks CB, Lal P, Zhang L, Coukos G
    Nature, 2011-07-13;475(7355):226-30.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: Neutralization
  8. Tumor-stromal crosstalk in invasion of oral squamous cell carcinoma: a pivotal role of CCL7.
    Authors: Jung DW, Che ZM, Kim J, Kim K, Kim KY, Williams D, Kim J
    Int. J. Cancer, 2010-07-15;127(2):332-44.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  9. Infiltrated neutrophils acquire novel chemokine receptor expression and chemokine responsiveness in chronic inflammatory lung diseases.
    Authors: Hartl D, Krauss-Etschmann S, Koller B, Hordijk PL, Kuijpers TW, Hoffmann F, Hector A, Eber E, Marcos V, Bittmann I, Eickelberg O, Griese M, Roos D
    J. Immunol., 2008-12-01;181(11):8053-67.
    Species: Human
    Sample Types: Whole Cells, Whole Tissue
    Applications: Flow Cytometry, ICC, IHC-P, Neutralization
  10. The in vitro migration capacity of human bone marrow mesenchymal stem cells: comparison of chemokine and growth factor chemotactic activities.
    Authors: Ponte AL, Marais E, Gallay N, Langonne A, Delorme B, Herault O, Charbord P, Domenech J
    Stem Cells, 2007-03-29;25(7):1737-45.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  11. Membrane-bound eotaxin-3 mediates eosinophil transepithelial migration in IL-4-stimulated epithelial cells.
    Authors: Yuan Q, Campanella GS, Colvin RA, Hamilos DL, Jones KJ, Mathew A, Means TK, Luster AD
    Eur. J. Immunol., 2006-10-01;36(10):2700-14.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  12. Functional analysis of the chemokine receptor CCR3 on airway epithelial cells.
    Authors: Beck LA, Tancowny B, Brummet ME, Asaki SY, Curry SL, Penno MB, Foster M, Bahl A, Stellato C
    J. Immunol., 2006-09-01;177(5):3344-54.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-P
  13. Increased expression of Th2-associated chemokines in bullous pemphigoid disease. Role of eosinophils in the production and release of these chemokines.
    Authors: Gounni Abdelilah S, Wellemans V, Agouli M, Guenounou M, Hamid Q, Beck LA, Lamkhioued B
    Clin. Immunol., 2006-06-16;120(2):220-31.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  14. Cytokine receptor-mediated trafficking of preformed IL-4 in eosinophils identifies an innate immune mechanism of cytokine secretion.
    Authors: Spencer LA, Melo RC, Perez SA, Bafford SP, Dvorak AM, Weller PF
    Proc. Natl. Acad. Sci. U.S.A., 2006-02-21;103(9):3333-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  15. The CC chemokine eotaxin/CCL11 has a selective profibrogenic effect on human lung fibroblasts.
    Authors: Puxeddu I, Bader R, Piliponsky AM, Reich R, Levi-Schaffer F, Berkman N
    J. Allergy Clin. Immunol., 2005-12-02;117(1):103-10.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC
  16. Plasmacytoid dendritic cell recruitment by immobilized CXCR3 ligands.
    Authors: Kohrgruber N, Groger M, Meraner P, Kriehuber E, Petzelbauer P, Brandt S, Stingl G, Rot A, Maurer D
    J. Immunol., 2004-12-01;173(11):6592-602.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  17. Possible link between unique chemokine and homing receptor expression at diagnosis and relapse location in a patient with childhood T-ALL.
    Authors: Annels NE, Willemze AJ, van der Velden VH, Faaij CM, van Wering E, Sie-Go DM, Egeler RM, van Tol MJ, Révész T
    Blood, 2003-12-04;103(7):2806-8.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  18. Phenotypic and functional analysis of T cells homing into the CSF of subjects with inflammatory diseases of the CNS.
    Authors: Giunti D, Borsellino G, Benelli R, Marchese M, Capello E, Valle MT, Pedemonte E, Noonan D, Albini A, Bernardi G, Mancardi GL, Battistini L, Uccelli A
    J. Leukoc. Biol., 2003-05-01;73(5):584-90.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  19. Histidyl-tRNA synthetase and asparaginyl-tRNA synthetase, autoantigens in myositis, activate chemokine receptors on T lymphocytes and immature dendritic cells.
    Authors: Howard OM, Dong HF, Yang D, Raben N, Nagaraju K, Rosen A, Casciola-Rosen L, Hartlein M, Kron M, Yiadom K, Dwivedi S, Plotz PH, Oppenheim JJ
    J. Exp. Med., 2002-09-16;196(6):781-91.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  20. Clonal Th2 cells associated with chronic hypereosinophilia: TARC-induced CCR4 down-regulation in vivo.
    Authors: de Lavareille A, Roufosse F, Schandene L, Stordeur P, Cogan E, Goldman M
    Eur. J. Immunol., 2001-04-01;31(4):1037-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CCR3 Antibody
By Anonymous on 10/01/2021
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Melanoma tissue Species: Human