Human CCR3 PerCP-conjugated Antibody

Catalog #: FAB155C Datasheet / COA / SDS

Clone 61828 was used by HLDA to establish CD designation

Catalog #	Availability	Size / Price	Qty
FAB155C-025
FAB155C-100

Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry.

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Human CCR3 PerCP-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR3 transfectants in flow cytometry. In flow cytometry, no cross-reactivity with CCR1, CCR2, CCR5, and CCR6 transfectants or the parental cell line is observed. Detects CCR3 on peripheral blood eosinophils.

Source

Monoclonal Rat IgG_2A Clone # 61828

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Y3 rat myeloid cell line transfected with human CCR3
Accession # P51677

Formulation

Supplied in a saline solution containing BSA and Sodium Azide.

Label

PerCP (Peridinin-chlorophyll Protein Complex) (Excitation= 482 and 564 nm, Emission= 675 nm)

Applications

Recommended Concentration

Sample

Flow Cytometry

10 µL/10⁶ cells

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CCR3 in Human Blood Granulocytes by Flow Cytometry. Human peripheral blood granulocytes were stained with Rat Anti-Human CCR3 PerCP-conjugated Monoclonal Antibody (Catalog # FAB155C) and Mouse Anti-Human Integrin a4/CD49d APC-conjugated Monoclonal Antibody (Catalog # FAB1354A). Quadrant markers were set based on control antibody staining (Catalog # IC006C). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze.

12 months from date of receipt, 2 to 8 °C as supplied.

Background: CCR3

CCR3 is a G protein-linked seven transmembrane chemokine receptor that preferentially binds eotaxin and eotaxin-2.

Entrez Gene IDs

1232 (Human); 12771 (Mouse); 117027 (Rat); 448802 (Canine)

Alternate Names

b-chemokine receptor; CC chemokine receptor 3; C-C chemokine receptor type 3; C-C CKR-3; CCR3; CCR-3; CD193 antigen; CD193; chemokine (C-C motif) receptor 3; CKR3CC-CKR-3CMKBR3CD193; eosinophil CC chemokine receptor 3; Eosinophil eotaxin receptor; MGC102841