Human CCR5 Antibody

Catalog #: MAB183
6 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB183-100
MAB183-SP

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All CCR5 Antibodies

Product Details

Citations (6)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCR5 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCR5.

Source

Monoclonal Mouse IgG_2B Clone # 45549

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CCR5
Met1-Leu352
Accession # P51681

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Immunocytochemistry

8-25 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Immunocytochemistry

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CCR5 in Human PBMCs. CCR5 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CCR5 Monoclonal Antibody (Catalog # MAB183) at 8 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCR5

CCR5 is a G protein-linked seven transmembrane domain chemokine receptor. CCR5 serves as a receptor for several chemokines including MIP-1 alpha, MIP-1 beta, MCP-2, and RANTES. It also functions as a coreceptor for Macrophage Tropic HIV-1 infection.

Entrez Gene IDs

1234 (Human); 12774 (Mouse); 117029 (Rat); 484789 (Canine); 493769 (Feline)

Alternate Names

C-C CKR-5; C-C motif chemokine receptor 5 A159A; CCCKR5; CC-CKR-5FLJ78003; CCR5; CCR-5; CD195 antigen; CD195; chemokine (C-C motif) receptor 5; chemokine receptor CCR5; chemr13; CKR5; CKR-5; HIV-1 fusion coreceptor; IDDM22CMKBR5C-C chemokine receptor type 5

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CCR5 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
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Preferential recognition of monomeric CCR5 expressed in cultured cells by the HIV-1 envelope glycoprotein gp120 for the entry of R5 HIV-1.
Authors: Nakano Y, Monde K, Terasawa H, Yuan Y, Yusa K, Harada S, Maeda Y
Virology, 2014-01-31;452(0):117-24.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CCR5 is a receptor for Staphylococcus aureus leukotoxin ED.
Authors: Alonzo, Francis, Kozhaya, Lina, Rawlings, Stephen, Reyes-Robles, Tamara, DuMont, Ashley L, Myszka, David G, Landau, Nathanie, Unutmaz, Derya, Torres, Victor J
Nature, 2012-12-12;493(7430):51-5.
Species: Human
Sample Types: Whole Cells
Applications: Bioassay
Expression of CCR5 receptors on Reed-Sternberg cells and Hodgkin lymphoma cell lines: involvement of CCL5/Rantes in tumor cell growth and microenvironmental interactions.
Authors: Aldinucci D, Lorenzon D, Cattaruzza L, Pinto A, Gloghini A, Carbone A, Colombatti A
Int. J. Cancer, 2008-02-15;122(4):769-76.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
CCL3 (MIP-1alpha) induces in vitro migration of GM-CSF-primed human neutrophils via CCR5-dependent activation of ERK 1/2.
Authors: Ottonello L, Montecucco F, Bertolotto M, Arduino N, Mancini M, Corcione A, Pistoia V, Dallegri F
Cell. Signal., 2005-03-01;17(3):355-63.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Invasive chronic inflammatory monocyte phenotype in subjects with high HIV-1 viral load.
Authors: Pulliam L, Sun B, Rempel H
J. Neuroimmunol., 2004-12-01;157(1):93-8.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Epitope mapping of CCR5 reveals multiple conformational states and distinct but overlapping structures involved in chemokine and coreceptor function.
Authors: Lee B, Sharron M, Blanpain C, Doranz BJ, Vakili J, Setoh P, Berg E, Liu G, Guy HR, Durell SR, Parmentier M, Chang CN, Price K, Tsang M, Doms RW
J. Biol. Chem., 1999-04-02;274(14):9617-26.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry