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Human CD11b/Integrin alpha M Antibody

Catalog #: MAB1699
4 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
MAB1699
MAB1699-SP
Detection of CD11b/Integrin alpha M in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (4)
FAQs
Supplemental Products
Reviews (1)
Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD11b/Integrin alpha M Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD11b/Integrin alpha M.
Source
Monoclonal Mouse IgG1 Clone # ICRF44
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Human rheumatoid synovial cells and monocytes
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Flow Cytometry
0.25 µg/106 cells
See below
Immunohistochemistry
Myones, B. et al. (1988) J. Clin Invest. 82: 640.  This clone is not recommended for paraffin-embedded sections.
 
CyTOF-reported
Miner, J.J. et al. (2015) Arthritis and Rheumatol. 67: 1214. Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
 
Immunocytochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD11b/Integrin alpha M antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CD11b/Integrin alpha M in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD11b/Integrin alpha M Monoclonal Antibody (Catalog # MAB1699, filled histogram) or isotype control antibody (Catalog # MAB002, open histogram), followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). View our protocol for Staining Membrane-associated Proteins.

Immunocytochemistry CD11b/Integrin aM antibody in Human PBMCs by Immunocytochemistry (ICC). View Larger

CD11b/Integrin alpha M in Human PBMCs. CD11b/Integrin aM was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using Mouse Anti-Human CD11b/Integrin aM Monoclonal Antibody (Catalog # MAB1699) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
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Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD11b/Integrin alpha M

The Integrin family proteins are heterodimeric transmembrane receptors composed of an alpha and a beta subunit. The Integrin alpha M subunit, also known as MAC-1 alpha subunit or CD11b, combines with the Integrin beta 2 subunit (CD18) to form the non-covalent heterodimer Integrin alpha M/ beta 2, also known as MAC-1 and complement receptor type 3 (CR3). Integrin alpha M/ beta 2 is expressed on granulocytes, macrophages, dendritic cells and natural killer cells. Upon activation, alpha M/ beta 2 can bind several ligands (including ICAM-1 fibrinogen and the C3 complement fragment C3bi) to mediate phagocyte adhesion, migration and ingestion of complement-opsonized particles.

Entrez Gene IDs
3684 (Human); 16409 (Mouse)
Alternate Names
antigen CD11b (p170); CD11 antigen-like family member B; CD11b antigen; CD11b; Cell surface glycoprotein MAC-1 subunit alpha; Complement component 3 receptor 3 subunit; CR-3 alpha chain; CR3A; CR3AMGC117044; Integrin alpha M; integrin alpha-M; integrin, alpha M (complement component 3 receptor 3 subunit); ITGAM; Leukocyte adhesion receptor MO1; MAC-1; MAC1A; macrophage antigen alpha polypeptide; MO1A; neutrophil adherence receptor alpha-M subunit; Neutrophil adherence receptor; SLEB6

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Citations for Human CD11b/Integrin alpha M Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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  1. Elucidating mechanisms of genetic cross-disease associations at the PROCR vascular disease locus
    Authors: D Stacey, L Chen, PJ Stanczyk, JMM Howson, AM Mason, S Burgess, S MacDonald, J Langdown, H McKinney, K Downes, N Farahi, JE Peters, S Basu, JS Pankow, W Tang, N Pankratz, M Sabater-Ll, PS de Vries, NL Smith, CHARGE Hem, AD Gelinas, DJ Schneider, N Janjic, NJ Samani, S Ye, C Summers, ER Chilvers, J Danesh, DS Paul
    Nature Communications, 2022-03-09;13(1):1222.
    Species: Human
    Sample Types: Whole Cells
    Applications: Neutralization
  2. Activation of the plasma kallikrein-kinin system on human lung epithelial cells.
    Authors: Vergiliana JF, Asokananthan N, Stewart GA
    Biol. Chem., 2010-09-01;391(9):1067-77.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  3. Cell-specific gene expression in Langerhans cell histiocytosis lesions reveals a distinct profile compared with epidermal Langerhans cells.
    Authors: Allen CE, Li L, Peters TL, Leung HC, Yu A, Man TK, Gurusiddappa S, Phillips MT, Hicks MJ, Gaikwad A, Merad M, McClain KL
    J. Immunol., 2010-03-10;184(8):4557-67.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC-Fr
  4. Aberration of CCR7 CD8 memory T cells from patients with systemic lupus erythematosus: an inducer of T helper type 2 bias of CD4 T cells.
    Authors: Sen Y, Chunsong H, Baojun H, Linjie Z, Qun L, San J, Qiuping Z, Junyan L, Zhang X, Jinquan T
    Immunology, 2004-06-01;112(2):274-89.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Reviews for Human CD11b/Integrin alpha M Antibody

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Human CD11b/Integrin alpha M Antibody
By Anonymous on 03/22/2022
Application: Immunocytochemistry/Immunofluorescence Sample Tested: Peripheral blood mononuclear cells (PBMCs) Species: Human
Immunocytochemistry/Immunofluorescence Human CD11b/Integrin alpha M Antibody MAB1699