Human CD133 Antibody

Catalog #: MAB11331
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11331-100
MAB11331-SP

Detection of CD133 in BG01V Human Cells by Flow Cytometry.

2 Images

All CD133 Antibodies

Product Details

Citations (3)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD133 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD133.

Source

Monoclonal Mouse IgG_2A Clone # 170411

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD133
Accession # O43490

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

BG01V human embryonic stem cells and iPS cells

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of CD133 in BG01V Human Cells by Flow Cytometry. BG01V human embryonic stem cells was stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (F0102B).

Flow Cytometry

View Larger

Detection of CD133 in iPS cells by Flow Cytometry. iPS cells were stained with Mouse Anti-Human CD133 Monoclonal Antibody (Catalog # MAB11331, filled histogram) or isotype control antibody (Catalog # MAB003, open histogram), followed by Phycoerythrin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0102B). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD133

CD133 antigen also known as prominin-1 is a glycoprotein that in humans is encoded by the PROM1 gene. It is a pentaspan transmembrane glycoprotein, with highly restricted expression on plasma membrane protrusions of epithelial and other cell types. CD133 is expressed in hematopoietic stem cells, endothelial progenitor cells, glioblastoma, neuronal and glial stem cells, as well as adult kidney, mammary glands, trachea, salivary glands, placenta, digestive tract, and testes. CD133 is used as a main marker for hematopoietic stem cells in transplant models. It has also been described as a marker of tumor initiating cells.

Entrez Gene IDs

8842 (Human); 19126 (Mouse)

Alternate Names

AC133 Stargardt disease 4 (autosomal dominant); AC133; CD133 retinal 2; CD133; PROM1; prominin (mouse)-like 1; Prominin 1; PROML1

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD133 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
Filter your results:

Filter by:

Multipurpose E-bioplatform targeting Kv channels in whole cancer cells and evaluating of their potential therapeutics
Authors: M Zouari, D Aissaoui-Z, S Campuzano, R Barderas, N Srairi-Abi, JM Pingarrón, N Raouafi
Analytica chimica acta, 2022-09-15;1231(0):340397.
Species: Human
Sample Types: Cell Culture Supernants
Applications: ELISA Capture
Clinical impact of different exosomes' protein expression in pancreatic ductal carcinoma patients treated with standard first line palliative chemotherapy
Authors: R Giampieri, F Piva, G Occhipinti, A Bittoni, A Righetti, S Pagliarett, A Murrone, F Bianchi, C Amantini, M Giulietti, G Ricci, G Principato, G Santoni, R Berardi, S Cascinu
PLoS ONE, 2019-05-02;14(5):e0215990.
Species: Human
Sample Types: Plasma
Applications: ELISA Detection
Musashi-1 promotes a cancer stem cell lineage and chemoresistance in colorectal cancer cells
Authors: GY Chiou, TW Yang, CC Huang, CY Tang, JY Yen, MC Tsai, HY Chen, N Fadhilah, CC Lin, YJ Jong
Sci Rep, 2017-05-19;7(1):2172.
Species: Human
Sample Types: Cell Lysates
Applications: Western Blot