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Human CD151 PE-conjugated Antibody

Catalog #: FAB1884P
1 Citation 1 Review Datasheet / COA / SDS
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FAB1884P
Detection of CD151 in Human Peripheral Blood Platelets by Flow Cytometry.
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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD151 PE-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD151.
Source
Monoclonal Mouse IgG2B Clone # 210127
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
NS0 mouse myeloma cell line transfected with human CD151
Met1-Tyr253
Accession # BAA06229
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Phycoerythrin (Excitation= 488 nm, Emission= 565-605 nm)

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD151 antibody in Human Peripheral Blood Platelets antibody by Flow Cytometry. View Larger

Detection of CD151 in Human Peripheral Blood Platelets by Flow Cytometry. Human peripheral blood platelets were stained with Mouse Anti-Human CD151 PE-conjugated Monoclonal Antibody (Catalog # FAB1884P) and FITC-conjugated anti-human CD41. Quadrant maker was set based on isotype control antibody staining (Catalog # IC0041P). View our protocol for Staining Membrane-associated Proteins.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store the unopened product at 2 - 8° C. Do not use past expiration date. Protect from light.

Background: CD151

CD151 is a tetraspanin superfamily glycoprotein expressed by endothelium, epithelia, megakaryocytes, and platelets. It associates with other tetraspanin proteins and with some integrins including alpha 3/ beta 1, alpha 6/ beta 1, alpha 6/ beta 4, and alpha 7/ beta 1. CD151 modulates cellular adhesion, cell migration, and platelet activation.

Entrez Gene IDs
977 (Human); 12476 (Mouse); 64315 (Rat)
Alternate Names
CD151 antigen (Raph blood group); CD151 antigenplatelet surface glycoprotein gp27; CD151 molecule (Raph blood group); CD151; GP27; Membrane glycoprotein SFA-1; MER2; PETA3; PETA-3; PETA-3SFA-1; platelet-endothelial cell tetraspan antigen 3; Platelet-endothelial tetraspan antigen 3; RAPH; SFA1; SFA-1; tetraspanin-24; TSPAN24; tspan-24; TSPAN24hemidesmosomal tetraspanin CD151

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Citation for Human CD151 PE-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. STAT3 phosphorylation at Ser727 and Tyr705 differentially regulates the EMT-MET switch and cancer metastasis
    Authors: WH Lin, YW Chang, MX Hong, TC Hsu, KC Lee, C Lin, JL Lee
    Oncogene, 2020-12-01;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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Human CD151 PE‑conjugated Antibody
By Anonymous on 10/26/2015
Application: Flow Sample Tested: Peripheral blood cells Species: Human

Gated on lymphocyte FSC-A and SSC-A populations. Singlets were further gated on by FSC-A/FSC-H doublet exclusion.
Specificity: Specific
Sensitivity: Reasonably sensitive
Buffer: 2% Human Serum, 0.5 mM EDTA in PBS
Dilution: 1/100

Flow Cytometry Human CD151 PE‑conjugated Antibody FAB1884P