Human CD163 Antibody

Name: Human CD163 Antibody
Brand: R&D Systems
SKU: MAB1607
Rating: 4 (4 reviews)

Catalog #: MAB1607
10 Citations 4 Reviews Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB1607-SP
MAB1607-100
MAB1607-500

All CD163 Antibodies

Product Details

Citations (10)

FAQs

Supplemental Products

Reviews (4)

Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD163 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD163 in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG₁ Clone # 215927

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Mouse myeloma cell line NS0-derived recombinant human CD163
Gly46-Ser1050
Accession # Q86VB7

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Purity

Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

Recombinant Human CD163 (Catalog # 1607-CD) under non-reducing conditions only

Flow Cytometry

2.5 µg/10⁶ cells

Human whole blood monocytes

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References

Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
Sulahian, T.H. et al. (2000) Cytokine 12:1312.
Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
Kristiansen, M. et al. (2001) Nature 409:198.
Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
Moller, H.J. et al. (2002) Blood 99:378.
Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
Schaer, D.J. et al. (2006) Blood 107:373.
Bover, L.C. et al. (2007) J. Immunol. 178:8183.
Fabriek, B.O. et al. (2007) Blood 109:5223.

Entrez Gene IDs

9332 (Human); 93671 (Mouse); 312701 (Rat)

Alternate Names

CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

Product Datasheets

Product Datasheet

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Related Research Areas

Citations for Human CD163 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

10 Citations: Showing 1 - 10
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Generation of cryopreserved macrophages from normal and genetically engineered human pluripotent stem cells for disease modelling
Authors: C Munn, S Burton, S Dickerson, K Bakshy, A Strouse, D Rajesh
PLoS ONE, 2021-04-22;16(4):e0250107.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Murlentamab, a Low Fucosylated Anti-Mï¿½llerian Hormone Type II Receptor (AMHRII) Antibody, Exhibits Anti-Tumor Activity through Tumor-Associated Macrophage Reprogrammation and T Cell Activation
Authors: M Prat, M Salon, T Allain, O Dubreuil, G Noël, L Preisser, B Jean, L Cassard, F Lemée, I Tabah-Fish, B Pipy, P Jeannin, JF Prost, JM Barret, A Coste
Cancers, 2021-04-13;13(8):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Induction of paracrine signaling in metastatic melanoma cells by PPAR? agonist rosiglitazone activates stromal cells and enhances tumor growth
Authors: C Pich, P Meylan, B Mastelic-G, NT Nguyen, R Loyon, BK Trang, H Moser, C Moret, C Goepfert, J Hafner, MP Levesque, P Romero, C Jandus, L Michalik
Cancer Res., 2018-09-05;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Human fetal dendritic cells promote prenatal T-cell immune suppression through arginase-2
Authors: N McGovern, A Shin, G Low, D Low, K Duan, LJ Yao, R Msallam, I Low, NB Shadan, HR Sumatoh, E Soon, J Lum, E Mok, S Hubert, P See, EH Kunxiang, YH Lee, B Janela, M Choolani, CNZ Mattar, Y Fan, TKH Lim, DK Hong, KK Tan, JKC Tam, C Schuster, A Elbe-Bürge, XN Wang, V Bigley, M Collin, M Haniffa, A Schlitzer, M Poidinger, S Albani, A Larbi, EW Newell, JKY Chan, F Ginhoux
Nature, 2017-06-14;0(0):.
Species: Human
Sample Types: Tissue Homogenates
Applications: Flow Cytometry
Jagged1 Instructs Macrophage Differentiation in Leprosy
PLoS Pathog, 2016-08-17;12(8):e1005808.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Endoneurial macrophages induce perineural invasion of pancreatic cancer cells by secretion of GDNF and activation of RET tyrosine kinase receptor.
Authors: Cavel O, Shomron O, Shabtay A, Vital J, Trejo-Leider L, Weizman N, Krelin Y, Fong Y, Wong R, Amit M, Gil Z
Cancer Res, 2012-09-12;72(22):5733-43.
Species: Human
Sample Types: Whole Cells
Applications: ICC
Glioma-associated cytomegalovirus mediates subversion of the monocyte lineage to a tumor propagating phenotype.
Authors: Dziurzynski K, Wei J, Qiao W, Hatiboglu MA, Kong LY, Wu A, Wang Y, Cahill D, Levine N, Prabhu S, Rao G, Sawaya R, Heimberger AB
Clin. Cancer Res., 2011-04-13;17(14):4642-9.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The class A scavenger receptor, macrophage receptor with collagenous structure, is the major phagocytic receptor for Clostridium sordellii expressed by human decidual macrophages.
Authors: Thelen T, Hao Y, Medeiros AI
J. Immunol., 2010-09-01;185(7):4328-35.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Human uterine epithelial cell secretions regulate dendritic cell differentiation and responses to TLR ligands.
Authors: Ochiel DO, Ghosh M, Fahey JV
J. Leukoc. Biol., 2010-04-12;88(3):435-44.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Generation of mature human myelomonocytic cells through expansion and differentiation of pluripotent stem cell-derived lin-CD34+CD43+CD45+ progenitors.
Authors: Choi KD, Vodyanik MA, Slukvin II
J. Clin. Invest., 2009-08-10;119(9):2818-29.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry