Human CD163 APC-conjugated Antibody

Catalog # Availability Size / Price Qty
FAB1607A-100
FAB1607A-025
Detection of CD163 in Human Blood Monocytes by Flow Cytometry.
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Product Details
Citations (8)
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Human CD163 APC-conjugated Antibody Summary

Species Reactivity
Human
Specificity
Detects human CD163 in direct ELISAs and Western blots.
Source
Monoclonal Mouse IgG1 Clone # 215927
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
Mouse myeloma cell line NS0-derived recombinant human CD163
Gly46-Ser1050
Accession # Q86VB7
Formulation
Supplied in a saline solution containing BSA and Sodium Azide.
Label
Allophycocyanin (Excitation= 620-650 nm, Emission= 660-670 nm)
Purity
Protein A or G purified from hybridoma culture supernatant

Applications

Recommended Concentration
Sample
Flow Cytometry
10 µL/106 cells
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry Detection of CD163 antibody in Human Blood Monocytes antibody by Flow Cytometry. View Larger

Detection of CD163 in Human Blood Monocytes by Flow Cytometry. Human peripheral blood monocytes were stained with Mouse Anti-Human CD163 APC-conjugated Monoclonal Antibody (Catalog # FAB1607A, filled histogram) or isotype control antibody (Catalog # IC002A, open histogram). View our protocol for Staining Membrane-associated Proteins.

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Preparation and Storage

Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
  • 12 months from date of receipt, 2 to 8 °C as supplied.

Background: CD163

CD163, previously called M130 or p155, is a 130‑160 kDa type I transmembrane glycoprotein that belongs to group B of the cysteine-rich scavenger receptor family (1‑3). It is essential for clearance of hemoglobin-haptoglobin (Hb-Hp) complexes in the liver, spleen and circulation (4). The human CD163 contains a 41 amino acid (aa) signal sequence, a 1009 aa extracellular domain (ECD) with 9 scavenger receptor cysteine-rich (SRCR) domains, a 22 aa transmembrane segment, and a 39‑84 aa cytoplasmic region (1). The third SRCR domain is crucial for calcium-dependent binding of hemoglobin/haptoglobin complexes (3). Three splice forms (isoforms 2, 3 and 4) vary within their intracellular regions (1, 5), while one isoform (# 4) also has a 34 aa insert between SRCR domains 5 and 6 within the ECD. While all are expressed, isoform 3 is the most abundant, being generally expressed on the cell surface and most active in endocytosis (5). An approximately 130 kDa soluble form of human CD163 (sCD163) is assumed to contain virtually all of the ECD, which shares 74%, 75%, 84%, 86%, 86% and 87% aa identity with mouse, rat, bovine, equine, porcine and canine CD163 ECD, respectively (6, 7). It is released from the cell surface by proteolysis after oxidative stress or inflammatory stimuli, including bacterial endotoxins and activation of the Toll-like receptors TLR2 or TLR5 (7‑10). Expression of CD163 is constitutive, and induced by glucocorticoids, IL‑10, IL‑6 or endotoxin on circulating monocytes, tissue macrophages, and at low levels on monocyte-derived dendritic cells (1, 2, 11, 12). In addition to clearing Hb‑Hp complexes, CD163 is also a scavenger receptor for free Hb (if Hp is depleted) and TWEAK (TNF-like weak inducer of apoptosis), and can function as an erythroblast adhesion receptor (4, 13‑15).

References
  1. Law, S.K.A. et al. (1993) Eur. J. Immunol. 23:2320.
  2. Sulahian, T.H. et al. (2000) Cytokine 12:1312.
  3. Madsen, M. et al. (2004) J. Biol. Chem. 279:51561.
  4. Kristiansen, M. et al. (2001) Nature 409:198.
  5. Nielsen, M.J. et al. (2006) J. Leukoc. Biol. 79:837.
  6. Moller, H.J. et al. (2002) Blood 99:378.
  7. Droste, A. et al. (1999) Biochem. Biophys. Res. Commun. 256:110.
  8. Hintz, K. A. et al. (2002) J. Leukoc. Biol. 72:711.
  9. Weaver, L.K. et al. (2006) J. Leukoc. Biol. 80:26.
  10. Timmerman, M. and P. Hogger (2005) Free Radic. Biol. Med. 39:98.
  11. Buechler, C. et al. (2000) J. Leukoc. Biol. 67:97.
  12. Pulford, K.A. et al. (1989) J. Clin. Pathol. 42:414.
  13. Schaer, D.J. et al. (2006) Blood 107:373.
  14. Bover, L.C. et al. (2007) J. Immunol. 178:8183.
  15. Fabriek, B.O. et al. (2007) Blood 109:5223.
Entrez Gene IDs
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163

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Citations for Human CD163 APC-conjugated Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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  1. Exosomal ?v?6 integrin is required for monocyte M2 polarization in prostate cancer
    Authors: H Lu, N Bowler, LA Harshyne, D Craig Hoop, SR Krishn, S Kurtoglu, C Fedele, Q Liu, HY Tang, AV Kossenkov, WK Kelly, K Wang, RB Kean, PH Weinreb, L Yu, A Dutta, P Fortina, A Ertel, M Stanczak, F Forsberg, DI Gabrilovic, DW Speicher, DC Altieri, LR Languino
    Matrix Biol., 2018-03-09;0(0):.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  2. Pleural Effusions from Patients with Mesothelioma Induce Recruitment of Monocytes and Their Differentiation into M2 Macrophages.
    Authors: Chene A, d'Almeida S, Blondy T, Tabiasco J, Deshayes S, Fonteneau J, Cellerin L, Delneste Y, Gregoire M, Blanquart C
    J Thorac Oncol, 2016-07-12;11(10):1765-73.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry, ICC
  3. Modulation of Decidual Macrophage Polarization by Macrophage Colony-Stimulating Factor Derived from First-Trimester Decidual Cells: Implication in Preeclampsia.
    Authors: Li M, Piao L, Chen C, Wu X, Yeh C, Masch R, Chang C, Huang S
    Am J Pathol, 2016-03-10;186(5):1258-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  4. A phase 1/2 study combining gemcitabine, Pegintron and p53 SLP vaccine in patients with platinum-resistant ovarian cancer.
    Authors: Dijkgraaf E, Santegoets S, Reyners A, Goedemans R, Nijman H, van Poelgeest M, van Erkel A, Smit V, Daemen T, van der Hoeven J, Melief C, Welters M, Kroep J, van der Burg S
    Oncotarget, 2015-10-13;6(31):32228-43.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  5. Ipilimumab-dependent cell-mediated cytotoxicity of regulatory T cells ex vivo by nonclassical monocytes in melanoma patients.
    Authors: Romano E, Kusio-Kobialka M, Foukas P, Baumgaertner P, Meyer C, Ballabeni P, Michielin O, Weide B, Romero P, Speiser D
    Proc Natl Acad Sci U S A, 2015-04-27;112(19):6140-5.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  6. Prolonged neutrophil dysfunction after Plasmodium falciparum malaria is related to hemolysis and heme oxygenase-1 induction.
    Authors: Cunnington, Aubrey J, Njie, Madi, Correa, Simon, Takem, Ebako N, Riley, Eleanor, Walther, Michael
    J Immunol, 2012-10-24;189(11):5336-46.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  7. Respiratory syncytial virus glycoprotein G interacts with DC-SIGN and L-SIGN to activate ERK1 and ERK2.
    Authors: Johnson TR, McLellan JS, Graham BS
    J. Virol., 2011-11-16;86(3):1339-47.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry
  8. Immunophenotypic characterization of human monocyte subsets: possible implications for cardiovascular disease pathophysiology.
    Authors: Shantsila E, Wrigley B, Tapp L, Apostolakis S, Montoro-Garcia S, Drayson MT, Lip GY
    J. Thromb. Haemost., 2011-05-01;9(5):1056-66.
    Species: Human
    Sample Types: Whole Cells
    Applications: Flow Cytometry

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