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Human CD163 Quantikine ELISA Kit

Catalog #: DC1630
42 Citations 1 Review Datasheet / COA / SDS
Catalog # Availability Size / Price Qty
DC1630
Control Products Available
QC61: Quantikine Immunoassay Control Set 1171 for Human CD163
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Human CD163 ELISA Standard Curve
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Citations (42)
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Summary Precision Recovery Linearity Data Examples Product Datasheets Preparation and Storage Background Related Research Areas

Human CD163 Quantikine ELISA Kit Summary

Assay Type
Solid Phase Sandwich ELISA
Format
96-well strip plate
Assay Length
4.5 hours
Sample Type & Volume Required Per Well
Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
Sensitivity
0.613 ng/mL
Assay Range
1.6 - 100 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
Specificity
Natural and recombinant human CD163
Cross-reactivity
< 0.5% cross-reactivity observed with available related molecules.< 50% cross-species reactivity observed with species tested.
Interference
No significant interference observed with available related molecules.

Product Summary

The Quantikine Human CD163 immunoassay is a 4.5 hour solid phase ELISA designed to measure human soluble CD163 in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human CD163 and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human CD163 showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human CD163.

Precision

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision

Cell Culture Supernates, Serum, Heparin Plasma

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean (pg/mL) 20 35.1 65.6 20 34.9 63.6
Standard Deviation 0.75 1.2 2.3 1.3 1.6 2.6
CV% 3.8 3.4 3.5 6.7 4.6 4.1

Recovery

The recovery of CD163 spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 101-110

Linearity

To assess the linearity of the assay, samples containing and/or spiked with high concentrations of CD163 were serially diluted with the Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human CD163 ELISA Linearity

Scientific Data

N/A Human CD163 ELISA Standard Curve View Larger

Human CD163 ELISA Standard Curve

Product Datasheets

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Preparation and Storage

Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Storage
Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: CD163

CD163, also known as M130 and p155, is a transmembrane scavenger receptor that is expressed on monocytes and macrophages and is inducible by immunosuppressant glucocorticoids and IL-10. A soluble form is shed from the cell surface by TACE or neutrophil elastase mediated cleavage in response to oxidative stress, Prostaglandin F2a stimulation, or the activation of Fc gamma receptors, TLR1, 2, 5, or 6. CD163 mediates monocyte binding to bacteria, leading to the release of inflammatory cytokines. It is essential for the circulatory clearance of hemoglobin-haptoglobin (Hb-Hp) complexes as well as free hemoglobin. It can also mediate monocyte-erythroblast adhesion and promote erythroblast expansion. CD163 binds and internalizes the cytokine TWEAK, and the ratio of soluble CD163 to TWEAK in the plasma is elevated during atherosclerosis.

Entrez Gene IDs:
9332 (Human); 93671 (Mouse); 312701 (Rat)
Alternate Names:
CD_antigen: CD163; CD163 molecule; CD163; GHI/61; HbSR; Hemoglobin scavenger receptor; M130; macrophage-associated antigen; MM130; RM3/1; SCARI1; scavenger receptor cysteine-rich type 1 protein M130; sCD163; Soluble CD163
⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Assay Procedure

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 50 µL Standard, Control, or Sample
  4.   Add 50 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Citations for Human CD163 Quantikine ELISA Kit

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

42 Citations: Showing 1 - 10
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  1. Anti-TIGIT antibody improves PD-L1 blockade through myeloid and Treg cells
    Authors: Guan, X;Hu, R;Choi, Y;Srivats, S;Nabet, BY;Silva, J;McGinnis, L;Hendricks, R;Nutsch, K;Banta, KL;Duong, E;Dunkle, A;Chang, PS;Han, CJ;Mittman, S;Molden, N;Daggumati, P;Connolly, W;Johnson, M;Abreu, DR;Cho, BC;Italiano, A;Gil-Bazo, I;Felip, E;Mellman, I;Mariathasan, S;Shames, DS;Meng, R;Chiang, EY;Johnston, RJ;Patil, NS;
    Nature
    Species: Human hepegivirus
    Sample Types: Serum
  2. HIV-associated gut microbial alterations are dependent on host and geographic context
    Authors: Rocafort, M;Gootenberg, DB;Luévano, JM;Paer, JM;Hayward, MR;Bramante, JT;Ghebremichael, MS;Xu, J;Rogers, ZH;Munoz, AR;Okello, S;Kim, JH;Sentongo, R;Wagubi, R;Lankowski, A;Maruapula, S;Zhao, G;Handley, SA;Mosepele, M;Siedner, MJ;Kwon, DS;
    Nature communications
    Species: Human hepegivirus
    Sample Types: Plasma
  3. Macrophage-associated markers of metaflammation are linked to metabolic dysfunction in pediatric obesity
    Authors: Lischka, J;Schanzer, A;de Gier, C;Greber-Platzer, S;Zeyda, M;
    Cytokine
    Species: Human
    Sample Types: Plasma
  4. Soluble CD163 is a predictor of fibrosis and hepatocellular carcinoma development in nonalcoholic steatohepatitis
    Authors: Kawanaka, M;Nishino, K;Kawada, M;Ishii, K;Tanikawa, T;Katsumata, R;Urata, N;Nakamura, J;Suehiro, M;Haruma, K;Kawamoto, H;
    BMC gastroenterology
    Species: Human
    Sample Types: Serum
  5. Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia
    Authors: Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert
    Brain, Behavior, and Immunity, 2023-03-25;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  6. Rebound HIV-1 in cerebrospinal fluid after antiviral therapy interruption is mainly clonally amplified R5 T cell-tropic virus
    Authors: LP Kincer, SB Joseph, MM Gilleece, BM Hauser, S Sizemore, S Zhou, C Di Germani, H Zetterberg, D Fuchs, SG Deeks, S Spudich, M Gisslen, RW Price, R Swanstrom
    Nature Microbiology, 2023-01-30;8(2):260-271.
    Species: Human
    Sample Types: CSF
  7. Increased prefrontal cortical cells positive for macrophage/microglial marker CD163 along blood vessels characterizes a neuropathology of neuroinflammatory schizophrenia
    Authors: Y Zhu, MJ Webster, AK Walker, P Massa, FA Middleton, CS Weickert
    Brain, Behavior, and Immunity, 2023;0(0):.
    Species: Human
    Sample Types: Tissue Homogenates
  8. Longitudinal multi-omics analyses of the gut-liver axis reveals metabolic dysregulation in hepatitis C infection and cirrhosis
    Authors: RO Ali, GM Quinn, R Umarova, JA Haddad, GY Zhang, EC Townsend, L Scheuing, KL Hill, M Gewirtz, S Rampertaap, SD Rosenzweig, AT Remaley, JM Han, V Periwal, H Cai, PJ Walter, C Koh, EB Levy, DE Kleiner, O Etzion, T Heller
    Nature Microbiology, 2022-12-15;0(0):.
    Species: Human
    Sample Types: Serum
  9. Long-Term Associations between Human Cytomegalovirus Antibody Levels with All-Cause Mortality and Cardiovascular Outcomes in an Australian Community-Based Cohort
    Authors: S Lee, N van den Be, A Castley, M Divitini, M Knuiman, P Price, D Nolan, F Sanfilippo, G Dwivedi
    Viruses, 2022-11-29;14(12):.
    Species: Human
    Sample Types: Serum
  10. The biological activity of serum bacterial lipopolysaccharides associates with disease activity and likelihood of achieving remission in patients with rheumatoid arthritis
    Authors: J Parantaine, G Barreto, R Koivuniemi, H Kautiainen, D Nordström, E Moilanen, M Hämäläinen, M Leirisalo-, K Nurmi, KK Eklund
    Arthritis Research & Therapy, 2022-11-21;24(1):256.
    Species: Human
    Sample Types: Serum
  11. Longitudinal Assessment of Multiple Immunological and Inflammatory Parameters during Successful DAA Therapy in HCV Monoinfected and HIV/HCV Coinfected Subjects
    Authors: P Zuccalà, T Latronico, R Marocco, S Savinelli, S Vita, F Mengoni, T Tieghi, C Borgo, B Kertusha, A Carraro, G D'Ettorre, V Vullo, CM Mastroiann, GM Liuzzi, M Lichtner
    International Journal of Molecular Sciences, 2022-10-08;23(19):.
    Species: Human
    Sample Types: Plasma
  12. Heme oxygenase-1 as an important predictor of the severity of COVID-19
    Authors: Y Hara, J Tsukiji, A Yabe, Y Onishi, H Hirose, M Yamamoto, M Kudo, T Kaneko, T Ebina
    PLoS ONE, 2022-08-24;17(8):e0273500.
    Species: Human
    Sample Types: Serum
  13. Influence of NAFLD and bariatric surgery on hepatic and adipose tissue mitochondrial biogenesis and respiration
    Authors: JS Pedersen, MO Rygg, K Chrøis, EG Sustarsic, Z Gerhart-Hi, NJ Wever Albr, RR Serizawa, VB Kristianse, AL Basse, AEB Boilesen, BH Olsen, T Hansen, LL Gluud, S Madsbad, S Larsen, F Bendtsen, F Dela
    Nature Communications, 2022-05-25;13(1):2931.
    Species: Human
    Sample Types: Plasma
  14. Inflammatory Markers after Switching to a Dual Drug Regimen in HIV-Infected Subjects: A Two-Year Follow-Up
    Authors: M Vassallo, J Durant, R Fabre, L Lotte, A Sindt, A Puchois, A De Monte, R Cezar, P Corbeau, C Pradier
    Viruses, 2022-04-28;14(5):.
    Species: Human
    Sample Types: Plasma
  15. Immune Activation in Primary Human Immunodeficiency Virus: Influence of Duration of Infection, Treatment, and Substance Use
    Authors: T Gilada, SR Schnittman, E White, J Mercader, Y Wang, S Dasgupta, R Valdez, D Pinto-Sant, S Pasalar, J Sanchez, P Gonzales, JR Lama, R Bender Ign, A Duerr
    Open forum infectious diseases, 2022-03-24;9(6):ofac155.
    Species: Human
    Sample Types: Plasma
  16. A matrix metalloproteinase-generated neoepitope of CRP can identify knee and multi-joint inflammation in osteoarthritis
    Authors: LC Alexander, G McHorse, JL Huebner, AC Bay-Jensen, MA Karsdal, VB Kraus
    Arthritis Research & Therapy, 2021-08-31;23(1):226.
    Species: Human
    Sample Types: Serum
  17. Assessing the differential impact of chronic CMV and treated HIV infection on CD8+ T-cell differentiation in a matched cohort study: is CMV the key?
    Authors: MC Mueller, WV Kern, S Usadel, MC Pauly, T Cathomen, U Salzer
    AIDS research and therapy, 2021-06-30;18(1):37.
    Species: Human
    Sample Types: Plasma
  18. Identification of distinct immune activation profiles in adult humans
    Authors: R Cezar, A Winter, D Desigaud, M Pastore, L Kundura, AM Dupuy, C Cognot, T Vincent, C Reynes, C Dunyach-Re, JP Lavigne, R Sabatier, P Le Merre, E Maggia, P Corbeau
    Scientific Reports, 2020-11-30;10(1):20824.
    Species: Human
    Sample Types: Plasma
  19. Impact of combined training with different exercise intensities on inflammatory and lipid markers in type 2 diabetes: a secondary analysis from a 1-year randomized controlled trial
    Authors: JP Magalhães, DA Santos, IR Correia, M Hetheringt, R Ribeiro, JF Raposo, A Matos, MD Bicho, LB Sardinha
    Cardiovasc Diabetol, 2020-10-07;19(1):169.
    Species: Human
    Sample Types: Plasma
  20. Interferon-alpha alters host glycosylation machinery during treated HIV infection
    Authors: LB Giron, F Colomb, E Papasavvas, L Azzoni, X Yin, M Fair, A Anzurez, M Damra, K Mounzer, JR Kostman, P Tebas, U O'Doherty, H Tateno, Q Liu, MR Betts, LJ Montaner, M Abdel-Mohs
    EBioMedicine, 2020-08-19;59(0):102945.
    Species: Human
    Sample Types: Plasma
  21. Sialylation and fucosylation modulate inflammasome-activating eIF2 Signaling and microbial translocation during HIV infection
    Authors: LB Giron, CE Tanes, MH Schleimann, PA Engen, LM Mattei, A Anzurez, M Damra, H Zhang, K Bittinger, F Bushman, A Kossenkov, PW Denton, H Tateno, A Keshavarzi, AL Landay, M Abdel-Mohs
    Mucosal Immunol, 2020-03-09;0(0):.
    Species: Human
    Sample Types: Plasma
  22. Factor Xa Inhibition Reduces Coagulation Activity but Not Inflammation Among People With HIV: A Randomized Clinical Trial
    Authors: JV Baker, J Wolfson, T Peterson, M Mooberry, M Gissel, H Mystakelis, MW Henderson, K Garcia-Mye, FS Rhame, TW Schacker, KE Brummel-Zi, I Sereti, NS Key, RP Tracy
    Open Forum Infect Dis, 2020-02-01;7(2):ofaa026.
    Species: Human
    Sample Types: Serum
  23. Differential expression and predictive value of monocyte scavenger receptor CD163 in populations with different tuberculosis infection statuses
    Authors: Q Liu, Q Ou, H Chen, Y Gao, Y Liu, Y Xu, Q Ruan, W Zhang, L Shao
    BMC Infect. Dis., 2019-11-28;19(1):1006.
    Species: Human
    Sample Types: Plasma
  24. Circulating monocytes accelerate acute liver failure by IL-6 secretion in monkey
    Authors: G Guo, Y Zhu, Z Wu, H Ji, X Lu, Y Zhou, Y Li, X Cao, Y Lu, P Talbot, J Liao, Y Shi, H Bu
    J. Cell. Mol. Med., 2018-07-11;0(0):.
    Species: Primate
    Sample Types: Serum
  25. Persistent high plasma levels of sCD163 and sCD14 in adult patients with measles virus infection
    Authors: C Mascia, I Pozzetto, B Kertusha, R Marocco, C Del Borgo, T Tieghi, S Vita, S Savinelli, M Iannetta, V Vullo, M Lichtner, CM Mastroiann
    PLoS ONE, 2018-05-24;13(5):e0198174.
    Species: Human
    Sample Types: Plasma
  26. Effect of influenza vaccine on tumor necrosis factor-like weak inducer of apoptosis (TWEAK) in older adults
    Authors: M Keshtkar-J, M Ouyang, M Keshtkarja, S Almed, H Li, JD Walston, R Rios, SX Leng
    Vaccine, 2018-03-13;0(0):.
    Species: Human
    Sample Types: Serum
  27. Serum levels of soluble CD163 and CXCL5 may be predictive markers for immune-related adverse events in patients with advanced melanoma treated with nivolumab: a pilot study
    Authors: T Fujimura, Y Sato, K Tanita, Y Kambayashi, A Otsuka, Y Fujisawa, K Yoshino, S Matsushita, T Funakoshi, H Hata, Y Yamamoto, H Uchi, Y Nonomura, R Tanaka, M Aoki, K Imafuku, H Okuhira, S Furudate, T Hidaka, S Aiba
    Oncotarget, 2018-02-15;9(21):15542-15551.
    Species: Human
    Sample Types: Serum
  28. Reevaluation of immune activation in the era of cART and an aging HIV-infected population
    Authors: LR de Armas, S Pallikkuth, V George, S Rinaldi, R Pahwa, KL Arheart, S Pahwa
    JCI Insight, 2017-10-19;2(20):.
    Species: Human
    Sample Types: Plasma
  29. Changes in inflammatory biomarkers in HCV-infected patients undergoing direct acting antiviral-containing regimens with or without interferon
    Authors: C Mascia, S Vita, P Zuccalà, R Marocco, T Tieghi, S Savinelli, R Rossi, M Iannetta, I Pozzetto, C Furlan, F Mengoni, CM Mastroiann, V Vullo, M Lichtner
    PLoS ONE, 2017-06-21;12(6):e0179400.
    Species: Human
    Sample Types: Plasma
  30. Macrophage-specific nanotechnology-driven CD163 overexpression in human macrophages results in an M2 phenotype under inflammatory conditions
    Authors: PA Alvarado-V, L Bernal, CA Paige, RL Grosick, C Moracho Vi, DW Ferreira, C Ulecia-Mor, EA Romero-San
    Immunobiology, 2017-05-16;0(0):.
    Species: Human
    Sample Types: Cell Culture Supernates
  31. Association of Anisocytosis with Markers of Immune Activation and Exhaustion in Treated HIV
    Authors: SG Al-Kindi, DA Zidar, GA McComsey, CT Longenecke
    Pathog Immun, 2017-05-02;2(1):138-150.
    Species: Human
    Sample Types: Plasma
  32. Immune Activation at Sites of HIV/TB Co-Infection Contributes to the Pathogenesis of HIV-1 Disease
    PLoS ONE, 2016-11-21;11(11):e0166954.
    Species: Human
    Sample Types: Plasma, Pleural Fluid
  33. Altered Monocyte and Endothelial Cell Adhesion Molecule Expression Is Linked to Vascular Inflammation in Human Immunodeficiency Virus Infection
    Authors: M Kulkarni, E Bowman, J Gabriel, T Amburgy, E Mayne, DA Zidar, C Maierhofer, AN Turner, JA Bazan, SL Koletar, MM Lederman, SF Sieg, NT Funderburg
    Open Forum Infect Dis, 2016-10-15;3(4):ofw224.
    Species: Human
    Sample Types: Plasma
  34. Late Antiretroviral Therapy (ART) Initiation Is Associated with Long-Term Persistence of Systemic Inflammation and Metabolic Abnormalities.
    Authors: Ghislain M, Bastard J, Meyer L, Capeau J, Fellahi S, Gerard L, May T, Simon A, Vigouroux C, Goujard C
    PLoS ONE, 2015-12-04;10(12):e0144317.
    Species: Human
    Sample Types: Serum
  35. Elevated plasma soluble CD14 and skewed CD16+ monocyte distribution persist despite normalisation of soluble CD163 and CXCL10 by effective HIV therapy: a changing paradigm for routine HIV laboratory monitoring?
    Authors: Castley, Alison, Berry, Cassandr, French, Martyn, Fernandez, Sonia, Krueger, Romano, Nolan, David
    PLoS ONE, 2014-12-29;9(12):e115226.
    Species: Human
    Sample Types: Plasma
  36. Coinfection with human herpesvirus 8 is associated with persistent inflammation and immune activation in virologically suppressed HIV-infected patients.
    Authors: Masia M, Robledano C, Ortiz de la Tabla V, Antequera P, Lumbreras B, Hernandez I, Gutierrez F
    PLoS ONE, 2014-08-18;9(8):e105442.
    Species: Human
    Sample Types: Plasma
  37. Reduced sTWEAK and increased sCD163 levels in HIV-infected patients: modulation by antiretroviral treatment, HIV replication and HCV co-infection.
    Authors: Beltran L, Munoz Hernandez R, de Pablo Bernal R, Garcia Morillo J, Egido J, Noval M, Ferrando-Martinez S, Blanco-Colio L, Genebat M, Villar J, Moreno-Luna R, Moreno J
    PLoS ONE, 2014-03-04;9(3):e90541.
    Species: Human
    Sample Types: Whole Blood
  38. Elevated levels of CXCL10 in the Periodic Fever, Aphthous stomatitis, Pharyngitis and cervical Adenitis syndrome (PFAPA) during and between febrile episodes; an indication of a persistent activation of the innate immune system.
    Authors: Forsvoll J, Kristoffersen E, Oymar K
    Pediatr Rheumatol Online J, 2013-10-17;11(1):38.
    Species: Human
    Sample Types: Plasma
  39. CD163 and IgG codefend against cytotoxic hemoglobin via autocrine and paracrine mechanisms.
    Authors: Subramanian K, Du R, Tan N, Ho B, Ding J
    J Immunol, 2013-04-15;190(10):5267-78.
    Species: Human
    Sample Types: Cell Culture Supernates
  40. Circulating levels of soluble MER in lupus reflect M2c activation of monocytes/macrophages, autoantibody specificities and disease activity.
    Authors: Zizzo G, Guerrieri J, Dittman L, Merrill J, Cohen P
    Arthritis Res Ther, 2013-01-01;15(6):R212.
    Species: Human
    Sample Types: Plasma
  41. Compensated inflammation in systemic juvenile idiopathic arthritis: role of alternatively activated macrophages.
    Authors: Shimizu M, Yachie A
    Cytokine, 2012-06-08;60(1):226-32.
    Species: Human
    Sample Types: Serum
  42. Peripheral artery disease is associated with a high CD163/TWEAK plasma ratio.
    Authors: Moreno JA, Dejouvencel T, Labreuche J, Smadja DM, Dussiot M, Martin-Ventura JL, Egido J, Gaussem P, Emmerich J, Michel JB, Blanco-Colio LM, Meilhac O
    Arterioscler. Thromb. Vasc. Biol., 2010-03-18;30(6):1253-62.
    Species: Human
    Sample Types: Plasma

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Human CD163 Quantikine ELISA Kit
By Anonymous on 12/15/2016
Sample Tested: EDTA Plasma

Plasma was isolated from blood drawn in EDTA tubes from HIV+/ART and HIV- healthy controls. sCD163 was measured using the Quantikine elisa kit for human sCD163 (cat# DC1630) following the protocol supplied in the kit. Plasma samples were diluted 1:10 before use in the assay.

Figure legends:
a) Standard curve for sCD163 from which concentrations in plasma were extrapolated
b) Quantitated data from HIV+/ART and HIV- group.

Concentrations are expressed in ng/ml.

Human CD163 Quantikine ELISA Kit DC1630