Human CD19 Antibody

Name: Human CD19 Antibody
Brand: R&D Systems
SKU: MAB4867
Rating: 4 (1 reviews)

Catalog #: MAB4867
5 Citations 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4867
MAB4867-SP

Detection of CD19 in Human PBMCs by Flow Cytometry.

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All CD19 Antibodies

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Citations (5)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD19 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD19.

Source

Monoclonal Mouse IgG₁ Clone # 4G7-2E3

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

Human CLL cells

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

0.25 µg/10⁶ cells

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

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Detection of CD19 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with Mouse Anti-Human CD3e PE‑conjugated Monoclonal Antibody (Catalog # FAB100P) and either (A) Mouse Anti-Human CD19 Monoclonal Antibody (Catalog # MAB4867) or (B) Mouse IgG₁Isotype Control (Catalog # MAB002) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B).

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD19

CD19 is a 95 kDa transmembrane glycoprotein with two Ig-like C2-set domains. CD19 regulates B cell development and activation through interactions with CD21, CD22, and the B cell receptor. CD19 polymorphisms and up‑regulation lead to the development of autoimmunity by promoting autoantibody production. Within the extracellular domain, human CD19 (Accession # P15391) shares 57% amino acid sequence identity with mouse and rat CD19.

Entrez Gene IDs

930 (Human); 12478 (Mouse); 365367 (Rat); 397669 (Porcine); 607898 (Canine); 102145514 (Cynomolgus Monkey); 100147468 (Equine)

Alternate Names

B4; B-lymphocyte antigen CD19; B-lymphocyte surface antigen B4; CD19 antigen; CD19 molecule; CD19; CVID3; Differentiation antigen CD19; Leu-12; MGC12802; T-cell surface antigen Leu-12

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD19 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Fluoxetine induces direct inhibitory effects on mesenchymal stem cell?derived osteoprogenitor cells independent of serotonin concentration
Authors: SM Koura, M Salama, M El-Hussiny, MEA Khalil, A Lotfy, SA Hassan, SA Gad Elhak, MA Sobh
Mol Med Rep, 2019-02-01;0(0):.
Species: Rat
Sample Types: Whole Cells
Applications: Flow Cytometry
Dysregulated CD46 shedding interferes with Th1-contraction in systemic lupus erythematosus
Authors: U Ellinghaus, A Cortini, CL Pinder, GL Friec, C Kemper, TJ Vyse
Eur. J. Immunol., 2017-05-22;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Exploiting light chains for the scalable generation and platform purification of native human bispecific IgG.
Authors: Fischer N, Elson G, Magistrelli G, Dheilly E, Fouque N, Laurendon A, Gueneau F, Ravn U, Depoisier J, Moine V, Raimondi S, Malinge P, Di Grazia L, Rousseau F, Poitevin Y, Calloud S, Cayatte P, Alcoz M, Pontini G, Fagete S, Broyer L, Corbier M, Schrag D, Didelot G, Bosson N, Costes N, Cons L, Buatois V, Johnson Z, Ferlin W, Masternak K, Kosco-Vilbois M
Nat Commun, 2015-02-12;6(0):6113.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
The effects of CAMPATH-1H on cell viability do not correlate to the CD52 density on the cell surface.
Authors: Lee F, Luevano M, Veys P, Yong K, Madrigal A, Shaw B, Saudemont A
PLoS ONE, 2014-07-22;9(7):e103254.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry
Role of MMPs in orchestrating inflammatory response in human monocytes via a TREM-1-PI3K-NF-kappaB pathway.
J. Cell. Sci., 2012-03-29;91(6):933-45.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry