Human CD20 Antibody

Catalog #: MAB4225
2 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB4225
MAB4225-SP

Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry.

2 Images

All CD20 Antibodies

Product Details

Citations (2)

Supplemental Products

Reviews

Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CD20 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CD20. Stains human CD20 transfectants but not irrelevant transfectants.

Source

Monoclonal Mouse IgG₁ Clone # 396444

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

NS0 mouse myeloma cell line transfected with human CD20
Met1-Pro297
Accession # P11836

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Flow Cytometry

2.5 µg/10⁶ cells

See below

Immunohistochemistry

5-25 µg/mL

See below

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Flow Cytometry

View Larger

Detection of MS4A1/CD20 in Human PBMCs by Flow Cytometry. Human peripheral blood mononuclear cells (PBMCs) were stained with either (A) Mouse Anti-Human MS4A1/CD20 Monoclonal Antibody (Catalog # MAB4225) or (B) Mouse IgG1 Isotype Control (MAB002) followed by anti-Mouse IgG APC-conjugated Secondary Antibody (F0101B) and Mouse Anti-Human CD19 PE-conjugated Monoclonal Antibody (FAB4867P). Staining was performed using our Staining Membrane-associated Proteins protocol.

Immunohistochemistry

View Larger

CD20 in Human Leukemia. CD20 was detected in immersion fixed paraffin-embedded sections of human leukemia using Mouse Anti-Human CD20 Monoclonal Antibody (Catalog # MAB4225) at 15 µg/mL for 1 hour at room temperature followed by incubation with the Anti-Mouse IgG VisUCyte™ HRP Polymer Antibody (Catalog # VC001). Tissue was stained using DAB (brown) and counterstained with hematoxylin (blue). Specific staining was localized to lymphocytes. View our protocol for IHC Staining with VisUCyte HRP Polymer Detection Reagents.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CD20

CD20 is a 297 amino acid (aa) non-glycosylated type III membrane protein that is the founding member of the MS4A tetraspanin protein family. It is expressed on pre-B, naïve and mature B lymphocytes and B-cell lymphomas. CD20 is associated with lipid rafts upon crosslinking, and appears to play a role in regulation of ion influx. The one extracellular segment of 48 aa shows 65% aa sequence identity between human and mouse CD20.

Long Name

Cluster of Differentiation 20

Entrez Gene IDs

931 (Human); 12482 (Mouse); 25316 (Rat); 485430 (Canine); 102118491 (Cynomolgus Monkey)

Alternate Names

B1; B-lymphocyte antigen CD20; B-lymphocyte cell-surface antigen B1; B-lymphocyte surface antigen B1; Bp35; Bp35MGC3969; CD20 antigen; CD20 receptor; CD20; CD20S7; CVID5; LEU-16; Leukocyte surface antigen Leu-16; Ly-44; Membrane-spanning 4-domains subfamily A member 1; membrane-spanning 4-domains, subfamily A, member 1; MS4A1; MS4A2; S7

Product Datasheets

Product Datasheet

COA

SDS

Related Research Areas

Citations for Human CD20 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

2 Citations: Showing 1 - 2
Filter your results:

Filter by:

Multiple sclerosis plasma IgG aggregates induce complement-dependent neuronal apoptosis
Authors: W Zhou, M Graner, P Paucek, C Beseler, M Boisen, A Bubak, F Asturias, W George, A Graner, D Ormond, T Vollmer, E Alvarez, X Yu
Cell Death & Disease, 2023-04-08;14(4):254.
Species: Human
Sample Types: Whole Cells
Applications: Neutralization
Preclinical development of a bispecific antibody that safely and effectively targets CD19 and CD47 for the treatment of B cell lymphoma and leukemia
Authors: V Buatois, Z Johnson, S Salgado-Pi, A Papaïoanno, E Hatterer, X Chauchet, F Richard, L Barba, B Daubeuf, L Cons, L Broyer, M D'Asaro, T Matthes, S LeGallou, T Fest, K Tarte, RK Clarke Hin, E Genescà Fe, JM Ribera, A Dey, K Bailey, AK Fielding, L Eissenberg, J Ritchey, M Rettig, JF DiPersio, MH Kosco-Vilb, K Masternak, N Fischer, L Shang, WG Ferlin
Mol. Cancer Ther., 2018-05-09;0(0):.
Species: Human
Sample Types: Whole Cells
Applications: Flow Cytometry